Poly(ADP-ribose) polymerase genes

Abstract

The invention relates to poly(ADP-ribose)polymerase (PARP) homologs which have an amino acid sequence which has a) a functional NAD binding domain and b) no zinc finger sequence motif of the general formula CX 2 CX m HX 2 C in which m is an integral value from 28 or 30, and the X radicals are, independently of one another, any amino acid; and the functional equivalents thereof; nucleic acids coding therefor; antibodies with specificity for the novel protein; pharmaceutical and gene therapy compositions which comprise products according to the invention; methods for the analytical determination of the proteins and nucleic acids according to the invention; methods for identifying effectors or binding partners of the proteins according to the invention; novel PARP effectors; and methods for determining the activity of such effectors.

We claim: 
     
       1. An isolated and purified nucleic acid consisting of a nucleotide sequence selected from the group consisting of nucleotides +3 to +1715 of SEQ ID NO:1, nucleotides +242 to +1843 of SEQ ID NO:3, nucleotides +221 to +1843 of SEQ ID NO:5, nucleotides +112 to +1710 of SEQ ID NO:7, and nucleotides +1 to +1584 of SEQ ID NO:9,
 wherein the nucleic acid codes for a poly(ADP-ribose) polymerase (PARP) that has poly(ADP-ribose)-synthesizing activity and no zinc finger motifs. 
 
     
     
       2. An expression cassette comprising, under the genetic control of at least one regulatory nucleotide sequence, at least one nucleic acid comprising a nucleotide sequence selected from the group consisting of nucleotides +3 to +1715 of SEQ ID NO:1, nucleotides +242 to +1843 of SEQ ID NO:3, nucleotides +221 to +1843 of SEQ ID NO:5, nucleotides +112 to +1710 of SEQ ID NO:7, and nucleotides +1 to +1584 of SEQ ID NO:9,
 wherein the nucleic acid codes for a poly(ADP-ribose) polymerase (PARP) that has poly(ADP-ribose)-synthesizing activity and no zinc finger motifs. 
 
     
     
       3. A recombinant vector comprising at least one expression cassette as claimed in  claim 2 . 
     
     
       4. A recombinant microorganism comprising at least one recombinant vector as claimed in  claim 3 .