Promoter of Corynebacteria

Abstract

An isolated nucleic acid comprising a promoter region having a nucleotide sequence of SEQ ID NO: 1, which can maintain high expression under aerobic and anaerobic conditions, and related compositions and methods.

What is claimed is: 
     
       1. An isolated recombinant nucleic acid comprising a promoter region having the nucleotide sequence of SEQ ID NO: 1. 
     
     
       2. The isolated recombinant nucleic acid of  claim 1 , wherein the isolated recombinant nucleic acid is a vector. 
     
     
       3. The isolated recombinant nucleic acid of  claim 1 , wherein a target gene is operably linked to the promoter region. 
     
     
       4. A host cell comprising a recombinant nucleic acid comprising a promoter region having the nucleotide sequence of SEQ ID NO: 1. 
     
     
       5. The host cell of  claim 4 , wherein the recombinant nucleic acid is a vector. 
     
     
       6. The host cell of  claim 4 , wherein a target gene is operably linked to the promoter region. 
     
     
       7. The host cell of  claim 4 , wherein the host cell is  Corynebacterium glutamicum.    
     
     
       8. A method of expressing a target gene, the method comprising culturing a host cell comprising a recombinant nucleic acid comprising a promoter region having the nucleotide sequence of SEQ ID NO: 1 and a target gene operably linked to the promoter region, thereby expressing the target gene. 
     
     
       9. The method of  claim 8 , wherein the host cell is  Corynebacterium glutamicum.    
     
     
       10. The method of  claim 9 , wherein the host cell is cultured under anaerobic conditions. 
     
     
       11. The method of  claim 10 , wherein expression of the target gene operably linked to the promoter region is greater than expression of the same gene is operably linked to a gapA promoter under the same conditions.