Procoagulant peptides and their derivatives and uses therefor

Abstract

The present invention is directed to a hemostatic or tissue sealing material having (a) a peptide having a sequence SEQ ID NO: 1 or an amino acid analog sequence thereof, and (b) a scaffold for said peptide or amino acid analog sequence. The scaffold is preferably hemostatic, such as a natural or genetically engineered absorbable polymer, a synthetic absorbable polymer, or combinations thereof. The natural or genetically engineered absorbable polymers can be selected from the group consisting of a protein, a polysaccharide, or combinations thereof.

We claim: 
     
       1. A hemostatic or tissue sealing material comprising:
 (a) a peptide sequence selected from SEQ ID NOs: 1-5 or their analog peptide sequence thereof, and 
 (b) a hemostatic scaffold, 
 wherein said hemostatic scaffold is a crosslinked gelatin in particle form with a liquid carrier. 
 
     
     
       2. The hemostatic or tissue sealing material of  claim 1  further comprising a normal saline solution, wherein gelatin and the peptide are substantially homogenously mixed in combination with the normal saline solution as a liquid phase. 
     
     
       3. The hemostatic or tissue sealing material of  claim 2  wherein concentration of said peptide in said hemostatic material is from about 0.0025 mM to about 1.25 mM. 
     
     
       4. The hemostatic or tissue sealing material of  claim 3  further comprising effective amounts of one or more additives or compounds selected from the group consisting of antimicrobial agents, surfactants, antioxidants, humectants, wetting agents, lubricants, thickeners, diluents, irradiation stabilizers, and combinations thereof. 
     
     
       5. The hemostatic or tissue sealing material of  claim 4  further comprising an extrusion enhancing amount of glycerol. 
     
     
       6. The hemostatic or tissue sealing material of  claim 1  wherein said peptide is conjugated to a biocompatible polymer. 
     
     
       7. The hemostatic or tissue sealing material of  claim 6  wherein said biocompatible polymer is a hydrophilic polymer. 
     
     
       8. The hemostatic or tissue sealing material of  claim 7  wherein said hydrophilic polymer is selected from the group consisting of polyethylene glycol, derivative of the polyethylene glycol, polypropylene glycol, polysaccharide, modified polysaccharide, protein, modified protein, peptide, polylactide glycolide, caprolactone, trimethylene carbonate, starch, modified starch, gelatin, collagen, and combinations thereof. 
     
     
       9. The hemostatic or tissue sealing material of  claim 7  wherein the hydrophilic polymer is polyethylene glycol having an average molecular weight selected from about 1000 Daltons to about 8000 Daltons to provide for better hemostatic efficacy than thrombin. 
     
     
       10. The hemostatic or tissue sealing material of  claim 9  wherein said polyethylene glycol molecule is a linear molecule, a branched molecule, a star-shaped molecule, or combinations thereof. 
     
     
       11. The hemostatic or tissue sealing material of  claim 10  wherein said molecular weight is on average 2000 Daltons, wherein said molecular weight is average molecular weight. 
     
     
       12. The hemostatic or tissue sealing material of  claim 10  wherein said molecular weight is on average 5000 Daltons, wherein said molecular weight is average molecular weight. 
     
     
       13. The hemostatic or tissue sealing material of  claim 10  wherein concentration of said peptide in said hemostatic material is from about 0.0025 mM to about 1.25 mM, and wherein said polyethylene glycol has a molecular weight from about 2000 to about 5000 Daltons.