Peptide specific for ovarian cancer and applications

Abstract

The present invention provides a novel peptide with high specificity for recognition of ovarian cancer. The peptide is selected using M13 phage display library and epithelial-enrich-conjugated magnetic beads. Only a small amount of the peptide is required to establish over 80% binding activity to ovarian cancer comparing to low binding activities to other cancers, showing high specificity for ovarian cancer. Thus the peptide of the present invention can be effective in early detection of ovarian cancer.

What is claimed is: 
     
       1. An isolated ovarian cancer-targeting peptide, comprising an amino acid sequence of SEQ ID NO: 1 encoded by a nucleotide sequence of SEQ ID NO: 2. 
     
     
       2. The peptide according to  claim 1 , wherein the peptide is conjugated to a magnetic particle. 
     
     
       3. A method of detecting or diagnosing ovarian cancer in a subject, comprising contacting a sample from the subject with the peptide according to  claim 1  under a condition, wherein the condition allows the peptide to connect to an ovarian cancer cell. 
     
     
       4. The method according to  claim 3 , further comprising conjugating a plurality of the peptides to a magnetic particle to form a peptide-magnetic particle complex. 
     
     
       5. The method according to  claim 4 , wherein the magnetic particle is a magnetic bead. 
     
     
       6. The method according to  claim 5 , wherein the binding activity of the peptide to the ovarian cancer cell is at least 80%. 
     
     
       7. The method according to  claim 5 , wherein the condition is the peptide concentration of 10 ρg/mL to 10 μg/mL. 
     
     
       8. The method according to  claim 5 , wherein the condition is the peptide concentration of 10 ρg/mL. 
     
     
       9. The method according to  claim 5 , wherein the peptide is conjugated to a detectable label, and the detectable label is a fluorophore, a chemiluminophore, a radioactive isotope, an enzyme, or a biotin. 
     
     
       10. A microfluidic chip, comprising the ovarian cancer-targeting peptide according to  claim 1 .