Cotton event pDAB4468.19.10.3 detection method

Abstract

Cotton event pDAB4468.19.10.3 comprises gene expression cassettes which contain genes encoding aad-12 and pat, affording herbicide tolerance to cotton crops containing the event, and enabling methods for crop protection. Embodiments of the subject invention provide polynucleotide-related event detection methods.

What is claimed is: 
     
       1. A method of detecting cotton event pDAB4468.19.10.3 in a sample comprising cotton DNA, the method comprising:
 contacting the sample with a first primer at least 10 bp in length that selectively binds to a flanking sequence within by 1-1354 of SEQ ID NO:1 or the complement thereof, and a second primer at least 10 bp in length that selectively binds to an insert sequence within by 1355-1672 of SEQ ID NO:1 or the complement thereof; and 
 assaying for an amplicon generated between the primers. 
 
     
     
       2. The method according to  claim 1 , wherein the sample is from at least one cotton plant comprising event pDAB4468.19.10.3, a representative sample of which has been deposited under ATCC accession number PTA-12457. 
     
     
       3. The method according to  claim 1 , the method further comprising:
 contacting said sample with a third primer at least 10 bp in length that selectively binds to an insert sequence within by 1-168 of SEQ ID NO:2 or the complement thereof, and a fourth primer at least 10 bp in length that selectively binds to flanking sequence within by 169-2898 of SEQ ID NO:2 or the complement thereof; and 
 assaying for an amplicon generated between the primers. 
 
     
     
       4. The method according to  claim 3 , wherein the sample is from at least one cotton plant comprising event pDAB4468.19.10.3, a representative sample of which has been deposited under ATCC accession number PTA-12457. 
     
     
       5. The method according to  claim 1 , wherein the first and second primers are selected from the group of SEQ ID NOs:5-7.