Anti-microbial peptides and methods of use thereof

Abstract

The present disclosure provides antimicrobial peptides, and compositions comprising same. The present disclosure further provides methods of inhibiting microbial growth.

What is claimed is: 
     
       1. A wound or surgical dressing comprising:
 i) a substrate; and 
 ii) an antimicrobial peptide bound to the substrate, wherein the antimicrobial peptide has a length of from 10 amino acids to 50 amino acids, wherein the antimicrobial peptide comprises an amino acid sequence having at least 95% amino acid sequence identity to a contiguous stretch of from 10 amino acids to 35 amino acids of the sequence YSYGSGLGVGGGFSSSSGRAIGGGLSSVGGGSSTIKYTTT SSSSR (SEQ ID NO:63), and wherein the antimicrobial peptide contains at least one phosphorylated tyrosine residue. 
 
     
     
       2. The wound or surgical dressing of  claim 1 , wherein the substrate comprises a gauze; an elastic material; a coform web; an airlaid web; a bonded carded web; a wetlaid web; a foam; a film; a woven fabric; a knitted fabric; or a hydroentangled web. 
     
     
       3. The wound or surgical dressing of  claim 1 , wherein the wound or surgical dressing comprises an adhesive bandage or a wrapping. 
     
     
       4. The wound or surgical dressing of  claim 1 , wherein the peptide comprises an amino acid sequence selected from: 
       
         
           
                 
                 
               
                     
                   (SEQ ID NO: 1) 
                 
                     
                   AIGGGLSSVGGGSSTIK; 
                 
                     
                     
                 
                     
                   (SEQ ID NO: 2) 
                 
                     
                   AIGGGLSSVGGGSSTIKY; 
                 
                     
                     
                 
                     
                   (SEQ ID NO: 3) 
                 
                     
                   RAIGGGLSSVGGGSSTIK; 
                 
                     
                     
                 
                     
                   (SEQ ID NO: 4) 
                 
                     
                   GGLSSVGGGSSTIK; 
                 
                     
                     
                 
                     
                   (SEQ ID NO: 5) 
                 
                     
                   AIGGGLSSVGGGS; 
                 
                     
                     
                 
                     
                   (SEQ ID NO: 6) 
                 
                     
                   RAIGGGLSSVGGGSSTIKY; 
                 
                     
                     
                 
                     
                   (SEQ ID NO: 7) 
                 
                     
                   YGSGLGVGGGFSSSSGRAIGGGLSSVGGGSSTIKY; 
                 
                     
                     
                 
                     
                   (SEQ ID NO: 8) 
                 
                     
                   YGSGLGVGGGFSSSSGRAIGGGLSSVGGGSSTIKYT;  
                 
                     
                   and 
                 
                     
                     
                 
                     
                   (SEQ ID NO: 9) 
                 
                     
                   GGLSSVGGGS. 
                 
             
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
               
            
           
         
       
     
     
       5. The wound or surgical dressing of  claim 1 , wherein at least two adjacent amino acids of the peptide are joined via a linkage other than an amide bond. 
     
     
       6. The wound or surgical dressing of  claim 1 , wherein the peptide comprises an amino acid sequence having at least 98% amino acid sequence identity to a contiguous stretch of from 10 amino acids to 35 amino acids of the sequence YSYGSGLGVGGGFSSSSGRAIGGGLSSVGGGSSTIKYTTTSSSSR (SEQ ID NO:63). 
     
     
       7. The wound or surgical dressing of  claim 1 , wherein the peptide comprises an amino acid sequence having at least 99% amino acid sequence identity to a contiguous stretch of from 10 amino acids to 35 amino acids of the sequence YSYGSGLGVGGGFSSSSGRAIGGGLSSVGGGSSTIKYTTTSSSSR (SEQ ID NO:63). 
     
     
       8. An implantable or insertable medical device comprising an antimicrobial peptide, wherein the antimicrobial peptide has a length of from 10 amino acids to 50 amino acids, wherein the antimicrobial peptide comprises an amino acid sequence having at least 95% amino acid sequence identity to a contiguous stretch of from 10 amino acids to 35 amino acids of the sequence YSYGSGLGVGGGFSSSSGRAIGGGLSSVGGGSSTIKYTTTSSSSR (SEQ ID NO:63), and wherein the antimicrobial peptide contains at least one phosphorylated tyrosine residue. 
     
     
       9. The implantable or insertable medical device of  claim 8 , wherein the implantable or insertable medical device is a contact lens. 
     
     
       10. The implantable or insertable medical device of  claim 8 , wherein the peptide comprises an amino acid sequence selected from: 
       
         
           
                 
                 
               
                     
                   (SEQ ID NO: 1) 
                 
                     
                   AIGGGLSSVGGGSSTIK; 
                 
                     
                     
                 
                     
                   (SEQ ID NO: 2) 
                 
                     
                   AIGGGLSSVGGGSSTIKY; 
                 
                     
                     
                 
                     
                   (SEQ ID NO: 3) 
                 
                     
                   RAIGGGLSSVGGGSSTIK; 
                 
                     
                     
                 
                     
                   (SEQ ID NO: 4) 
                 
                     
                   GGLSSVGGGSSTIK; 
                 
                     
                     
                 
                     
                   (SEQ ID NO: 5) 
                 
                     
                   AIGGGLSSVGGGS; 
                 
                     
                     
                 
                     
                   (SEQ ID NO: 6) 
                 
                     
                   RAIGGGLSSVGGGSSTIKY; 
                 
                     
                     
                 
                     
                   (SEQ ID NO: 7) 
                 
                     
                   YGSGLGVGGGFSSSSGRAIGGGLSSVGGGSSTIKY; 
                 
                     
                     
                 
                     
                   (SEQ ID NO: 8) 
                 
                     
                   YGSGLGVGGGFSSSSGRAIGGGLSSVGGGSSTIKYT;  
                 
                     
                   and 
                 
                     
                     
                 
                     
                   (SEQ ID NO: 9) 
                 
                     
                   GGLSSVGGGS. 
                 
             
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
               
            
           
         
       
     
     
       11. The implantable or insertable medical device of  claim 8 , wherein at least two adjacent amino acids of the peptide are joined via a linkage other than an amide bond. 
     
     
       12. The implantable or insertable medical device of  claim 8 , wherein the peptide comprises an amino acid sequence having at least 98% amino acid sequence identity to a contiguous stretch of from 10 amino acids to 35 amino acids of the sequence YSYGSGLGVGGGFSSSSGRAIGGGLSSVGGGSSTIKYTTTSSSSR (SEQ ID NO:63). 
     
     
       13. The implantable or insertable medical device of  claim 8 , wherein the peptide comprises an amino acid sequence having at least 99% amino acid sequence identity to a contiguous stretch of from 10 amino acids to 35 amino acids of the sequence YSYGSGLGVGGGFSSSSGRAIGGGLSSVGGGSSTIKYTTTSSSSR (SEQ ID NO:63).