Protective solutions for organs

Abstract

Described is a protective solution for avoiding ischemic, storage or ischemia/reperfusion to organs, or to isolated cell systems, or to tissue components after perfusion, surgery, transplantation, or cryopreservation and subsequent reperfusion, which contains alkali ions, and if need be also alkaline earth ions as the electrolyte, a buffer e.g. on a histidine derivation basis, as well as a polyol and/or a saccharide, has an osmolarity of about 290 mosm/l to about 350 mosm/l, as well as a pH value of about 6.8 to about 7.4, and to which hydroxamic acid, and/or one or more hydroxamic acid derivatives are added.

The invention claimed is: 
     
       1. A protective solution for avoiding ischemic damage to organs, or to isolated cell systems, or to tissue components after perfusion, surgery, transplantation, or cryoconservation and subsequent reperfusion, which solution comprises the following components:
 (i) sodium at a concentration of about 10 mmol/l to about 120 mmol/l; 
 (ii) magnesium at a concentration of about 3 mmol/l to about 27 mmol/l; 
 (iii) calcium at a concentration of about 0.0001 mmol/l to about 1.5 mmol/l; 
 (iv) chloride; 
 (v) n-acetyl histidine; 
 (vi) sucrose; 
 (vii) a-ketoglutarate at a concentration of about 1 mmol/l to about 9 mmol/l; 
 (viii) aspartate in an amount in excess of chloride; 
 (ix) glycine; 
 (x) arginine; 
 (xi) deferoxamine; and 
 (xii) 3,4-dimethoxy-N-methyl-benzohydroxamic acid; 
 
       wherein said solution has an osmolarity of about 290 Osm/l to about 350 mOsm/l, as well as a pH value of about 6.8 to about 7.4; 
       wherein the maximum concentration of aspartate is about 140 mmol/l; 
       wherein the maximum concentration of arginine is about 140 mmol/l; 
       wherein the maximum concentration of deferoxamine is about 10 mmol/l; and 
       wherein the maximum concentration of 3,4-dimethoxy-N-methyl-benzohydroxamic acid is about 10 mmol/l. 
     
     
       2. The solution according to  1 , wherein the solution contains potassium ions at a concentration of about 5 mmol/l to about 25 mmol/l. 
     
     
       3. A protective solution for avoiding ischemic damage to organs, or to isolated cell systems, 
       or to tissue components after perfusion, surgery, transplantation, or cryoconservation and subsequent reperfusion, which solution comprises the following components:
 (i) sodium at a concentration of about 10 mmol/l to about 120 mmol/l; 
 (ii) magnesium at a concentration of about 3 mmol/l to about 27 mmol/l; 
 (iii) calcium at a concentration of about 0.0001 mmol/l to about 1.5 mmol/l; 
 (iv) chloride; 
 (v) n-acetyl histidine; 
 (vi) sucrose; 
 (vii) a-ketoglutarate at a concentration of about 1 mmol/l to about 9 mmol/l; 
 (viii) aspartate; 
 (ix) glycine; 
 (x) arginine; 
 (xi) deferoxamine; and 
 (xii) 3,4-dimethoxy-N-methyl-benzohydroxamic acid; 
 
       wherein said solution has an osmolarity of about 290 mOsm/l to about 350 mOsm/l, as well as a pH value of about 6.8 to about 7.4. 
     
     
       4. A solution comprising:
 (i) sodium at a concentration of about 10 mmol/l to about 120 mmol/l; 
 (ii) magnesium at a concentration of about 3 mmol/l to about 27 mmol/l; 
 (iii) calcium at a concentration of about 0.0001 mmol/l to about 1.5 mmol/l; 
 (iv) chloride; 
 (v) n-acetyl histidine; 
 (vi) sucrose; 
 (vii) a-ketoglutarate at a concentration of about 1 mmol/l to about 9 mmol/l; 
 (viii) aspartate in an amount in excess of chloride; 
 (ix) glycine; 
 (x) arginine; 
 (xi) deferoxamine; and 
 (xii) 3,4-dimethoxy-N-methyl-benzohydroxamic acid; 
 
       wherein said solution has an osmolarity of about 290 mOsm/l to about 350 mOsm/l, as well as a pH value of about 6.8 to about 7.4; 
       wherein the maximum concentration of aspartate is about 140 mmol/l; 
       wherein the maximum concentration of arginine is about 140 mmol/l; 
       wherein the maximum concentration of deferoxamine is about 10 mmol/l; and 
       wherein the maximum concentration of 3,4-dimethoxy-N-methyl-benzohydroxamic acid is about 10 mmol/l. 
     
     
       5. A solution comprising:
 (i) sodium at a concentration of about 10 mmol/l to about 120 mmol/l; 
 (ii) magnesium at a concentration of about 3 mmol/l to about 27 mmol/l; 
 (iii) calcium at a concentration of about 0.0001 mmol/l to about 1.5 mmol/l; 
 (iv) chloride; 
 (v) n-acetyl histidine; 
 (vi) sucrose; 
 (vii) a-ketoglutarate at a concentration of about 1 mmol/l to about 9 mmol/l; 
 (viii) aspartate; 
 (ix) glycine; 
 (x) arginine; 
 (xi) deferoxamine; and 
 (xii) 3,4-dimethoxy-N-methyl-benzohydroxamic acid; 
 
       wherein said solution has an osmolarity of about 290 mOsm/l to about 350 mOsm/l, as well as a pH value of about 6.8 to about 7.4.