Reaction vessel for sample preparation

Abstract

Disclosed is a sample preparation container for purification and/or enrichment of bio-organic compounds from cellular material, viruses and/or sub-components of these. The container includes a reaction chamber and a chromatography medium. The reaction chamber is for holding the cellular material, etc. and is configured to perform reactions inside. The chromatography medium is configured to purify the bio-organic compounds. The chromatography medium is located at a wall of the reaction chamber, and the wall is closed or sealed and configured to be opened for obtaining purified bio-organic compounds. The sample preparation container further includes a receiving chamber for receiving the bio-organic compounds, that is adjacent to the chromatography medium such that the chromatography medium separates the reaction chamber from the receiving chamber. The outer face of the receiving chamber is closed and configured to be opened for obtaining purified bio-organic compounds.

The invention claimed is: 
     
       1. A method of preparing purified and/or enriched bio-organic compounds from cellular material, viruses and/or sub-components of said cellular material and/or viruses, said method comprising
 (a) introducing said cellular material, viruses and/or sub-components of said cellular material and/or viruses, comprising proteins, into a reaction chamber of a container, said container further comprising a chromatographic medium and a receiving chamber, said receiving chamber being adjacent to said chromatography medium, said chromatographic medium separating said reaction chamber from said receiving chamber, wherein an outer face of said receiving chamber is closed and configured to be opened for obtaining said purified and/or enriched bio-organic compounds; 
 (b) disrupting said cellular material, viruses and/or sub-components of said cellular material and/or viruses inside said reaction chamber; 
 (c) performing proteolytic digestion; 
 (d) eluting said bio-organic compounds, 
 thereby obtaining said purified and/or enriched bio-organic compounds in said receiving chamber; 
 
       wherein the bio-organic compounds comprise at least one of peptides, polypeptides, nucleic acids, lipids, or metabolites; wherein said disrupting is effected by
 (i) sonication; 
 (ii) boiling in the presence of a chaotropic agent and/or a denaturing agent; and/or 
 (iii) bead milling; 
 and 
 wherein said method is exclusively performed in said container. 
 
     
     
       2. The method of  claim 1 , wherein said disrupting is effected by bead milling. 
     
     
       3. The method of  claim 1 , wherein step (c) of said method further comprises reduction and alkylation.