N-acetyl-D-glucosamine deacetylase and a process for preparing the same

Abstract

There are provided N-acetyl-D-glucosamine deacetylase capable of hydrolyzing acetamide group of N-acetyl-D-glucosamine to produce D-glucosamine and acetic acid, which has the following physicochemical properties: (1) Substrate specificity: N-acetyl-D-glucosamine monomer, not the oligomer or polymer thereof; (2) Optimal pH: 7.8-8.2 at 37° C.; (3) Stable pH: 6.0-9.0 at 45° C.; (4) Optimal temperature: 28°-39° C.; (5) Molecular weight: 70,000-200,000 as determined using TSK-gel G-3000 SW column, and a process for preparing the same using a microorganism belonging to Vibrio sp.

What is claimed is: 
     
       1. N-acetyl-D-glycosamine deacetylase capable of hydrolyzing the acetamide group of N-acetyl-D-glycosamine to produce D-glycosamine and acetic acid, which is obtained from Vibrio cholereae non-01 (IFO 15429) and which has the following physicochemical properties: substrate specificity: N-acetyl-D-glycosamine monomer;   optimal pH: 7.8-8.2 at 37° C.;   stable pH: 6.0-9.0 at 45° C.;   optimal temperature: 28°-39° C.; and   molecular weight: 70,000-200,000.   
     
     
       2. N-acetyl-D-glucosamine deacetylase as claimed in claim 1 which has a molecular weight of 80,000-150,000.