USH1531HExpiredUtilityPatentIndex 90

Thermophilic DNA polymerase

Priority: Apr 18, 1994Filed: Apr 18, 1994Granted: May 7, 1996

Est. expiryApr 18, 2014(expired)· nominal 20-yr term from priority

Inventors:BLUMENTALS ILSE I LASKEN ROGER S SCHMIDT BRIAN J LONGO MARY C HUGHES JR A JOHN CHATTERJEE DEB K

C12N 9/1252

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Abstract

The invention relates to a substantially pure thermostable DNA polymerase. Preferably, the DNA polymerase has a molecular weight of about 95 kilodaltons and is more thermostable than Taq DNA polymerase. The present invention also relates to cloning and expression of the DNA polymerase in E. coli, to DNA molecules containing the cloned gene, and to host cells which express said genes.

Claims

exact text as granted — not AI-modified

What is claimed is: 
     
       1. A substantially pure DNA polymerase comprising the amino acid sequence set forth in SEQ ID NO:2. 
     
     
       2. A substantially pure DNA polymerase comprising the amino acid sequence set forth in SEQ ID NO:2 except wherein asparagine at position 141 has been replaced by alanine and glutamine at position 143 has been replaced by alanine.

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