Dry-type glucose analyzing element

Abstract

A dry-type quantitative glucose-analyzing element comprising a water-impermeable light-transmissive support having thereon a group of water-permeable layers comprising a reagent layer, an enzyme-containing layer, and a porous spreading layer which are laminated on said support in this order, and a hydrogen peroxide-detecting coloring composition comprising a hydrogen donor and a coupler; wherein said reagent layer contains at least said coupler; said enzyme-containing layer contains a glucose oxidase, a peroxidase and a mordant which immobilizes a dye formed by said coloring composition; and said hydrogen donor is contained in at least one water-permeable layer of said group. Using the element, quantitative determination of glucose of high concentration is possible with high accuracy.

What is claimed is: 
     
       1. A dry-type quantitative glucose-analyzing element comprising a water-impermeable light-transmissive support having thereon a group of water-permeable layers comprising a reagent layer, an enzyme-containing layer, and a porous spreading layer which are laminated on said support in this order, and a hydrogen peroxide-detecting coloring composition comprising a hydrogen donor and a coupler; wherein said reagent layer contains at least said coupler;   said enzyme-containing layer contains a glucose oxidase, a peroxidase and a mordant which immobilizes a dye formed by said coloring composition; and   said hydrogen donor is contained in at least one water-permeable layer of said group.   
     
     
       2. A dry quantitative glucose-analyzing element as in claim 1, wherein said group further comprises a light-shielding layer between the enzyme-containing layer and the spreading layer. 
     
     
       3. A dry quantitative glucose-analyzing element as in claim 2, wherein said light-shielding layer is oxygen-permeable and protein-impermeable. 
     
     
       4. A dry quantitative glucose-analyzing element as in claim 1, wherein said hydrogen donor is contained in at least one of the reagent layer and the enzyme-containing layer. 
     
     
       5. A dry quantitative glucose-analyzing element as in claim 4, wherein said hydrogen donor is contained in the enzyme-containing layer. 
     
     
       6. A dry quantitative glucose-analyzing element as in claim 4, wherein said hydrogen donor is contained in the reagent layer. 
     
     
       7. A dry quantitative glucose-analyzing element as in claim 2, wherein said hydrogen donor is contained in at least one of the reagent layer and the enzyme-containing layer. 
     
     
       8. A dry quantitative glucose-analyzing element as in claim 1, wherein said enzyme containing layer contains glucose oxidase in an amount of from 5,000 to 70,000 units/m 2 . 
     
     
       9. A dry quantitative glucose-analyzing element as in claim 1, wherein said enzyme containing layer contains peroxidase in an amount of from 10,000 to 150,000 units/m 2 . 
     
     
       10. A dry quantitative glucose-analyzing element as in claim 1, wherein said enzyme containing layer contains said mordant in an amount of from 0.3 to 6 g/m 2 . 
     
     
       11. A dry quantitative glucose-analyzing element as in claim 1, wherein said enzyme containing layer has a thickness of from 2 to 30 μm.