Process for purifying 2',3'-dideoxynucleosides

Abstract

A biological process for producing a 2',3'-dideoxynucleoside from 2',3'-dideoxyuridine is disclosed. The 2',3'-dideoxynucleoside can be purified readily using a porous nonpolar resin absorbent.

What is claimed as new and desired to be secured by Letters Patent of the United States is: 
     
       1. A process for the purification of a 2',3'-dideoxynucleoside from a solution obtained from a process requiring a microorganism or an enzyme, said solution containing, as impurities, other 2',3'-dideoxynucleosides or nucleic acid bases, said process comprising: (i) contacting said solution with a porous, nonpolar resin to cause the adsorption of said 2',3'-dideoxynucleoside onto said porous nonpolar resin, wherein said 2',3'-dideoxynucleoside is 2',3'-dideoxyadenosine or 2',3'-dideoxyinosine;   (ii) separating said porous nonpolar resin from said solution; and   (iii) fractionally eluting said adsorbed 2',3'-dideoxynucleoside to obtain a purified 2',3'-dideoxynucleoside product.   
     
     
       2. The process of claim 1, wherein said porous nonpolar resin is a styrene-divinylbenzene resin. 
     
     
       3. The process of claim 1, wherein said porous nonpolar resin is a modified styrene-divinylbenzene resin. 
     
     
       4. The process of claim 1, wherein said 2',3'-dideoxynucleoside is 2',3'-dideoxyadenosine. 
     
     
       5. The process of claim 4, wherein said porous nonpolar resin is a styrene-divinylbenzene resin. 
     
     
       6. The process of claim 4, wherein said porous nonpolar resin is a modified styrene-divinylbenzene resin. 
     
     
       7. The process of claim 1, wherein said 2',3'-dideoxynucleoside is 2',3'-dideoxyinosine. 
     
     
       8. The process of claim 7, wherein said porous nonpolar resin is a styrene-divinylbenzene resin. 
     
     
       9. The process of claim 7, wherein said porous nonpolar resin is a modified styrene-divinylbenzene resin. 
     
     
       10. The process of claim 1, said solution containing, as impurities, said other 2',3'-dideoxynucleosides and said nucleic acid bases. 
     
     
       11. The process of claim 4, said solution containing, as impurities, uracil and 2',3'-dideoxyuridine. 
     
     
       12. The process of claim 7, said solution containing, as impurities, uracil and 2',3'-dideoxyuridine. 
     
     
       13. The process of claim 4, said solution containing, as impurities, adenine, uracil and 2',3'-dideoxyuridine. 
     
     
       14. The process of claim 7, said solution containing, as impurities, uracil, 2',3'-dideoxyuridine and hypoxanthine. 
     
     
       15. The process of claim 4, said solution containing, as impurities, adenine. 
     
     
       16. The process of claim 7, said solution containing as impurities, hypoxanthine.