Production of insecticidal protein of Bacillus thuringiensis subsp. aizawai by the expression of insecticidal protein gene in host cells

Abstract

Insecticidal protein of B. thuringiensis subsp. aizawai IPL is produced by growing a transformant microorganism harboring a recombinant expression vector carrying a gene coding for the insecticidal protein.

We claim: 
     
       1. A plasmid carrying a DNA sequence coding for the insecticidal protein of Bacillus thuringiensis subsp. aizawai IPL given in FIG. 2. 
     
     
       2. A plasmid according to claim 1, which is named as pAB6. 
     
     
       3. A recombiant expression vector carrying a DNA sequence coding for the insecticidal protein of Bacillus thuringiensis subsp. aizawai IPL given in FIG. 2, and being capable of producing said insecticidal protein in host cells. 
     
     
       4. A recombinant expression vector according to claim 3, wherein the DNA sequence is as given in FIG. 2. 
     
     
       5. A recombinant vector according to claim 3, which is selected from the group consisting of pAH8, pAH7 and pTB1. 
     
     
       6. A microorganism carrying a recombinant plasmid containing a DNA sequence coding for the insecticidal protein of Bacillus thuringiensis subsp. aizawai IPL given in FIG. 2. 
     
     
       7. A microorganism according to claim 6, which belongs to E. coli. 
     
     
       8. A microorganism according to claim 6, wherein the recombinant plasmid is pAB6, pAH8, pAH7 or pTB1. 
     
     
       9. A process for producing the insecticidal protein of Bacillus thuringiensis subsp. aizawai IPL which comprises: culturing a microorganism carrying a recombinant expression vector harboring a gene coding for the protein described in FIG. 2 in a suitable medium; and   collecting the produced protein.   
     
     
       10. A plasmid which is pTB1. 
     
     
       11. A microorganism carrying a recombinant plasmid, wherein said microorganism belongs to E. coli, and wherein said recombinant plasmid is pTB1. 
     
     
       12. A microorganism which is E. coli JM103/pTB1. 
     
     
       13. A DNA encoding for the insecticidal protein of FIG. 2. 
     
     
       14. The DNA of claim 13 comprising a DNA sequence wherein said DNA sequence is that of FIG. 2. 
     
     
       15. A DNA sequence as given in FIG. 2, coding for the insecticidal protein of Bacillus thuringiensis sub-species aizawai IPL having the amino acid sequence given in FIG. 2.