Method to clean and disinfect pathogens on the epidermis by applying a composition containing peroxidase, an iodide compound, a peroxide and a surfactant

Abstract

This invention relates to a disinfecting epidermal cleaner using peroxidase, peroxide and iodide. The active components are maintained inactive until admixed in a [define] defined proportion with water. The pH at which the peroxidase is stored is between 7.0 and 9.0 and the pH of the admixture of the active components is between 3.0 and 6.5.

What is claimed is: 
     
       1. A method for topically treating skin to simultaneously clean and disinfect pathogens on the epidermis comprising the steps of: (a) combining a peroxidase with an iodide compound .[.which will provide a source of iodide ions in water to form a first component.]. .Iadd.to form a first component.Iaddend.;   (b) combining a source of peroxide with .[.a surface active agent(s).]. .Iadd.at least one surface active agent .Iaddend.selected from the .[.class.]. .Iadd.group .Iaddend.consisting of anionic, cationic, zwitterionic, non-ionic and ampholytic .[.molecules.]. .Iadd.surface active agents .Iaddend.to form a second component;   .[.(c) adding buffer means to either said first or second components, or to both said first and second components, to control the pH of an aqueous admixture of said first and second components in a range of between a pH of 3.0 and about 6.5;   (d) admixing said buffered first and second components with a defined volume of water such that a buffered aqueous admixture is formed within said pH range, with said buffered first and second components diluted between about fifty percent (50%) to one thousand percent (1000%); and.].   .Iadd.(c) admixing said first and second component with water to form an aqueous admixture;   (d) adding a buffer to said first or second component prior to step (c), or to the aqueous admixture formed in step c to maintain a pH in said aqueous admixture between a pH of 3.0 and about 6.5; and .Iaddend.   (e) contacting the surface of the skin to be treated with said aqueous admixture.   
     
     
       2. A method, as defined in claim 1, wherein said .[.first component is an aqueous solution comprising a.]. peroxidase .Iadd.is .Iaddend.selected from the Enzyme Commission identification No. E.C..[.,.]. 1.11.1.7.[., a buffering agent, a carrier and said iodide compound.].. 
     
     
       3. A method, as defined in claim 2, wherein said iodide compound is a salt of iodide in a concentration of 0.00010 and 1300 mg/ml of said first component. 
     
     
       4. A method, .[.a.]. .Iadd.as .Iaddend.defined in claim 3, wherein .[.the pH of.]. said first component .[.is adjusted.]. .Iadd.includes water and a buffer to adjust its pH .Iaddend.to between a pH of 7.0 and 9.0. 
     
     
       5. A method, as defined in claim 4, .[.wherein said carrier is.]. .Iadd.further comprising a carrier .Iaddend.selected from the class consisting of sucrose, ethylene gylcol, glycerol, and .[.other.]. polyhydroxylated alkanes. 
     
     
       6. A method, as defined in claim 4, wherein said .Iadd.source of .Iaddend.peroxide in said second component lies in a concentration of between 0.0003 and 3.0 percent weight to volume relative to said surface active agent(s). 
     
     
       7. A method for .Iadd.topically treating skin to .Iaddend.simultaneously .[.cleaning and disinfecting.]. .Iadd.clean and disinfect .Iaddend.pathogens on the epidermis .[.in the treatment of bovine mastitis.]. comprising the steps of: forming an aqueous, antiseptic solution consisting essentially of: a source of .[.hydrogen.]. peroxide, an iodide compound which forms iodide ions in water, a peroxidase selected from the Enzyme Commission Classification No. 1.11.17, buffer means, surface active agents, and water, with the concentration of said buffer means sufficient to maintain the pH of the solution between a pH of 3 and 6.5, .[.and with the concentration of water representing from 50 percent to ten times the combined volume of the peroxidase, iodide, and peroxide components;.]. and   contacting the epidermal area to be treated with said aqueous solution.