Cellular attachment to trans-epithelial appliances

Abstract

A trans-epithelial appliance having a hemidesmosome formation-inducing protein composition derived from rat bladder carcinoma cells deposited thereon. This composition stimulates cell attachment and may be either the cell matrix or a soluble factor isolated from the conditioned medium. The appliance will be useful for diminishing inflammation and/or infection at the site of entry of the appliance. The appliance may also be used to stimulate gum junctional epithelium adhesion in the treatment of gingivitis and periodontitis. The composition may be used to maintain tissues ex vivo.

What is claimed is: 
     
       1. An article of manufacture comprising a trans-epithelial appliance coated with a soluble hemidesmosome formation-inducing factor obtainable from 804G or NBT-II rat bladder carcinoma cells, wherein the factor induces epithelial cell attachment-to the coated trans-epithelial appliance. 
     
     
       2. The article of claim 1, wherein said appliance is a shaped article selected from the group consisting of indwelling catheter, needle, metal pin, metal rod, colostomy tube, dental abutment piece and surgical mesh. 
     
     
       3. The article of claim 1, further comprising epithelial cells deposited on said hemidesmosome formation-inducing composition. 
     
     
       4. The article of claim 1, wherein said appliance is used in vivo. 
     
     
       5. The article of claim 1, wherein said appliance is made of or coated with a biocompatible metal. 
     
     
       6. The article of claim 5, wherein said metal is stainless steel or titanium. 
     
     
       7. The article of claim 1, wherein said appliance is made of or coated with a ceramic material. 
     
     
       8. The article of claim 7, wherein said material is hydroxyapatite. 
     
     
       9. The article of claim 1, wherein said appliance is made of or coated with a polymer. 
     
     
       10. The article of claim 9, wherein said polymer is selected from the group consisting of polyester, polyglycolic acid and a polygalactose-polyglycolic acid copolymer. 
     
     
       11. A method for inducing epithelial cell attachment to a trans-epithelial .[.appliance.]. .Iadd.surface, .Iaddend.comprising the following steps: (a) coating a trans-epithelial .[.appliance.]. .Iadd.surface .Iaddend.with a soluble hemidesmosome formation-inducing factor obtainable from 804G or NBT-II rat bladder carcinoma cells, and   (b) incubating the coated trans-epithelial .[.appliance.]. .Iadd.surface .Iaddend.with epithelial cells under conditions necessary to induce epithelial cell attachment to the coated trans-epithelial .[.appiance.]. .Iadd.surface.Iaddend..   
     
     
       12. The method of claim 11, wherein said .[.appliance.]. .Iadd.surface .Iaddend.is selected from the group consisting of .Iadd.the surface of an .Iaddend.indwelling catheter, .Iadd.a .Iaddend.needle, .Iadd.a .Iaddend.metal pin, .Iadd.a .Iaddend.metal rod, .Iadd.a .Iaddend.colostomy tube, .Iadd.a .Iaddend.dental abutment piece and .Iadd.a .Iaddend.surgical mesh. 
     
     
       13. The method of claim 11, wherein said .[.appliance.]. .Iadd.surface .Iaddend.is made of or coated with a polymer. 
     
     
       14. The method of claim 13, wherein said polymer is selected from the group consisting of polyester, polyglycolic acid and a polygalactose-polyglycolic acid copolymer. .Iadd.15. The method of claim 11, wherein said surface is the surface of a tooth. .Iaddend..Iadd.16. The method of claim 15, wherein said tooth is in an area affected with periodontitis or gingivitis and said soluble factor promotes adhesion of periodontal tissue to said tooth. .Iaddend..Iadd.17. The method of claim 16, wherein said soluble factor is produced by recombinant means. .Iaddend..Iadd.18. The method of claim 16, wherein said periodontal tissue is gingival tissue or gum junctional epithelium. .Iaddend.