Expression cassette for antisense expression of ribozyme

Abstract

The invention is an expression cassette for the antisense expression of ribozyme, having a strong promotor, suitably a T7 promotor, an adenoviral va-RNA gene, a stable loop region, and an insertion site for the antisense/ribozyme sequence in the loop region.

We claim:  
     
       1. An antisense and ribozyme expression cassette which comprises a strong promotor, an adenoviral va-RNA gene having a central part, a stable loop region of a size of at least 2×21 base pairs of identical sequences, and an insertion site for the antisense/ribozyme sequence in the loop region, wherein said promotor, the adenoviral va-RNA gene, the loop region, and the insertion site are linked as shown in FIG.  1 . 
     
     
       2. The expression cassette of claim  1 , wherein said promotor is a T7 promotor. 
     
     
       3. The expression cassette of claim  2 , wherein the T7 promotor is employed in combination with a T7 polymerase. 
     
     
       4. The expression cassette of claim  1 , wherein said loop region is in a restriction site in the central part of the adenoviral va-RNA gene. 
     
     
       5. The expression cassette of claim  1 , wherein said loop region contains twice the base sequence 5′-AACCCAGGTGTGCGACGTCAG-3′ (Seq. Id. No. 1).