USRE38792EExpiredUtilityPatentIndex 72
Modified bacterial cellulose
Est. expiryJul 26, 2016(expired)· nominal 20-yr term from priority
C12P 19/04
72
PatentIndex Score
9
Cited by
8
References
63
Claims
Abstract
This invention provides a bacterial cellulose comprising ribbon-shaped microfibrils having a thickness of 10 to 100 nm and a width of 160 to 1000 nm or a bacterial cellulose comprising ribbon-shaped microfibrils having a thickness of 10 to 100 nm and a width of 50 to 70 nm. The former bacterial cellulose can be produced by culturing cellulose-producing bacteria in a culture medium containing a cell division inhibitor, and the latter can be produced by culturing the bacterium in a culture medium containing an organic reducing agent. The bacterial cellulose is modified from conventional bacterial cellulose in the major axis, and is improved in Young's modulus, etc.This invention provides a bacterial cellulose comprising ribbon- shaped microfibrils having a thickness of 1 to 9 nm and a width of 160 to 1000 nm or a bacterial cellulose comprising ribbon - shaped microfibrils having a thickness of 1 to 9 nm and a width of 50 to 70 nm. The former bacterial cellulose can be produced by culturing cellulose - producing bacteria in a culture medium containing a cell division inhibitor, and the latter can be produced by culturing the bacterium in a culture medium containing an organic reducing agent. The bacterial cellulose is modified from conventional bacterial cellulose in the major axis, and is improved in Young's modulus, etc.
Claims
exact text as granted — not AI-modified1. An isolated and purified bacterial cellulose comprising microfibrils having a thickness of 10 to 100 nm and a width of 250 to 1000 nM.
2. The bacterial cellulose of claim 1 , which has a width of 250 to 700 nm.
3. The bacterial cellulose of claim 1 , which has a width of 250 to 600 nm.
4. The bacterial cellulose of claim 1 , which has a width of 170 to 1000 nm.
5. The bacterial cellulose of claim 1 , which has a width of 250 to 700 nm.
6. The bacterial cellulose of claim 1 , which has a width of 250 to 600 nm.
7. The bacterial cellulose of claim 1 , wherein the microfibrils are ribbon-shaped.
8. A method of producing the bacterial cellulose of claim 1 , which comprises culturing cellulose-producing bacteria which produce the bacterial cellulose extracellularly in a culture medium containing a cell division inhibitor, and recovering the bacterial cellulose produced in the culture medium.
9. The method of claim 8 , wherein the cell division inhibitor is selected from the group consisting of chloramphenicol, a protein synthesis inhibitor, an organic compound having β-lactamase inhibiting ability, nalidixic acid, promidic acid, pipemidic acid, oxolinaic acid, ofloxacin and enoxacin.
10. The method of claim 9 , wherein the protein synthesis inhibitor is selected from the group consisting of tetracycline, puromycin and erythromycin.
11. The method of claim 9 , wherein the organic compound having β-lactamase inhibiting ability is thienamycin.
12. The method of claim 8 , wherein the concentration of the cell division inhibitor in the culture medium is 0.01 to 5 mM.
13. The method of claim 8 , wherein the bacteria are Acetobacter.
14. The method of claim 8 , wherein the bacteria are Acetobacter pasteurianus FERM BP-4176.
15. The bacterial cellulose of claim 1 , which has a width of 430 to 1000 nm.
16. The bacterial cellulose of claim 1 , which has a width of 590 to 1000 nm.
17. The bacterial cellulose of claim 1 , which has a Young's modulus of 13 to 20 GPa.
18. The bacterial cellulose of claim 1 , which has a Young's modulus of 16 to 20 GPa.
19. The bacterial cellulose of claim 1 , which has a width of 340 to 1000 nm.
20. The bacterial cellulose of claim 1 , which has a width of 340 to 700 nm.
21. The bacterial cellulose of claim 1 , which has a width of 340 to 600 nm.
22. A bacterial cellulose comprising microfibrils having a thickness of 1 to 9 nm and a width of 250 to 1000 nm.
23. The bacterial cellulose of claim 22 , wherein the microfibrils have a width of 250 to 700 nm.
24. The bacterial cellulose of claim 22 , wherein the microfibrils have a width of 250 to 600 nm.
25. The bacterial cellulose of claim 22 , wherein the microfibrils are ribbon- shaped.
26. A method of producing the bacterial cellulose of claim 22 , which comprises culturing cellulose- producing bacteria which produce the bacterial cellulose extracellularly in a culture medium containing a cell division inhibitor, and recovering the bacterial cellulose produced in the culture medium.
27. The method of claim 26 , wherein the cell division inhibitor is selected from the group consisting of chloramphenicol, a protein synthesis inhibitor, an organic compound having β- lactamase inhibiting ability, nalidixic acid, promidic acid, pipemidic acid, oxolinaic acid, ofloxacin and enoxacin.
28. The method of claim 27 , wherein the protein synthesis inhibitor is selected from the group consisting of tetracycline, puromycin and erythromycin.
29. The method of claim 27 , wherein the organic compound having β- lactamase inhibiting ability is thienamycin.
30. The method of claim 26 , wherein the concentration of the cell division inhibitor in the culture medium is 0 . 01 to 5 mM.
31. The method of claim 26 , wherein the bacteria are Acetobacter.
32. The method of claim 26 , wherein the bacteria are Acetobacter pasteurianus FERM BP- 4176 .
33. The bacterial cellulose of claim 22 , wherein the microfibrils have a width of 430 to 1000 nm.
34. The bacterial cellulose of claim 22 , wherein the microfibrils have a width of 590 to 1000 nm.
35. The bacterial cellulose of claim 22 , wherein the microfibrils have a Young's modulus of about 13 to 20 GPa.
36. The bacterial cellulose of claim 22 , wherein the microfibrils have a Young's modulus of about 16 to 20 Gpa.
37. The bacterial cellulose of claim 22 , wherein the microfibrils have a width of 340 to 1000 nm.
38. The bacterial cellulose of claim 22 , wherein the microfibrils have a width of 340 to 700 nm.
39. The bacterial cellulose of claim 22 , wherein the microfibrils have a width of 340 to 600 nm.
40. The bacterial cellulose of claim 22 , wherein the microfibrils have a thickness of 2 . 5 , 3 , 6 , or 9 nm.
41. The bacterial cellulose of claim 22 , wherein the ratio of the major axis to the minor axis of the microfibrils is about 28 : 1 . 0 to 1000 : 1 . 0 .
42. The bacterial cellulose of claim 22 , wherein the ratio of the major axis to the minor axis of the microfibrils is about 28 : 1 . 0 to 280 : 1 . 0 .
43. The bacterial cellulose of claim 22 , which is isolated and purified.
44. A bacterial cellulose produced by Acetobacter pasteurianus FERM BP- 4176 which comprises microfibrils having a thickness of 1 to 9 nm and a width of 250 to 1000 nm.
45. The bacterial cellulose of claim 44 , wherein the microfibrils have a width of 250 to 700 nm.
46. The bacterial cellulose of claim 44 , wherein the microfibrils have a width of 250 to 600 nm.
47. The bacterial cellulose of claim 44 , wherein the microfibrils have a width of 430 to 1000 nm.
48. The bacterial cellulose of claim 44 , wherein the microfibrils have a width of 590 to 1000 nm.
49. The bacterial cellulose of claim 44 , wherein the microfibrils have a width of 340 to 1000 nm.
50. The bacterial cellulose of claim 44 , wherein the microfibrils have a width of 340 to 700 nm.
51. The bacterial cellulose of claim 44 , wherein the microfibrils have a width of 340 to 600 nm.
52. The bacterial cellulose of claim 44 , wherein the microfibrils have a Young's modulus of about 13 to 20 GPa.
53. The bacterial cellulose of claim 44 , wherein the microfibrils have a Young's modulus of about 16 to 20 Gpa.
54. The bacterial cellulose of claim 44 , wherein the ratio of the major axis to the minor axis of the microfibrils is about 28 : 1 . 0 to 1000 : 1 . 0 .
55. The bacterial cellulose of claim 44 , wherein the ratio of the major axis to the minor axis of the microfibrils is about 28 : 1 . 0 to 280 : 1 . 0 .
56. The bacterial cellulose of claim 44 , wherein the microfibrils are ribbon- shaped.
57. A method of producing the bacterial cellulose of claim 44 , which comprises culturing cellulose- producing bacteria which produce the bacterial cellulose extracellularly in a culture medium containing a cell division inhibitor, and recovering the bacterial cellulose produced in the culture medium.
58. The method of claim 57 , wherein the cell division inhibitor is selected from the group consisting of chloramphenicol, a protein synthesis inhibitor, an organic compound having β- lactamase inhibiting ability, nalidixic acid, promidic acid, pipemidic acid, oxolinaic acid, ofloxacin and enoxacin.
59. The method of claim 58 , wherein the protein synthesis inhibitor is selected from the group consisting of tetracycline, puromycin and erythromycin.
60. The method of claim 58 , wherein the organic compound having β- lactamase inhibiting ability is thienamycin.
61. The method of claim 57 , wherein the concentration of the cell division inhibitor in the culture medium is 0 . 01 to 5 mM.
62. The method of claim 57 , wherein the bacteria are Acetobacter.
63. The method of claim 57 , wherein the bacteria are Acetobacter pasteurianus FERM BP- 4176 .Cited by (0)
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