Methods and apparatus for synthesizing labeled combinatorial chemistry libraries

Abstract

The present invention provides labeled synthetic libraries of random oligomers and methods and apparatus for generating labeled synthetic oligomer libraries. Each member of such a library is labeled with a unique identifier tag that specifies the structure or sequence of the oligomer. In a preferred embodiment of the present invention the identifier tag is a microchip that is pre-encoded or encodable with information that is related back to a detector when the identifier tag is pulsed with electromagnetic radiation.

1. A pre-encoded substrate useful for being linked to a single oligomer structure whereby said pre-encoded substrate bears a unique identifier tag, said tag being a pre-encoded microchip identifying said oligomer structure. 
     
     
       2. An encodable substrate useful for being linked to a single oligomer structure whereby said encodable substrate has a unique identifier tag, said tag being a encodable microchip identifying said oligomer structure. 
     
     
       3. One or a plurality of the pre-encoded substrate of  claim 1  useful for in a method of preparing a labeled synthetic oligomer library comprising a plurality of different members, each member comprising a distinct pre-encoded substrate whereby said one or plurality of pre-encoded substrate is linked to a oligomer structure said method comprising the steps of:
 a) apportioning said pre-encoded substrates among a plurality of reaction vessels;  
 b) exposing said pre-encoded substrates in each reaction vessel to one or a plurality of transformation events;  
 c) detecting and recording identifier information for each of said identifier tags in each of said reaction vessels;  
 d) apportioning said pre-encoded substrates among a plurality of reaction vessels; and  
 e) repeating steps a) through c) from at least one to about twenty times wherein said preencoded substrate being a pre-encoded microchip.  
 
     
     
       4. One or a plurality of the encodable substrate of  claim 2  useful in a method of preparing a labeled synthetic oligomer library comprising a plurality of different members, each member comprising a distinct encodable substrate whereby said one or plurality of pre-encoded substrate a oligomer structure is linked to a oligomer structure said method comprising the steps of:
 a) apportioning said encodable substrates among a plurality of reaction vessels;  
 b) exposing said encodable substrates in each reaction vessels to one or a plurality of transformation events;  
 c) adding first identifier information to said encodable substrates;  
 d) apportioning said encodable substrates among a plurality of reaction vessels; and  
 e) repeating steps a) through c) from at least one to about twenty times wherein said encodable substrate being a encodable microchip.  
 
     
     
       5. One or a plurality of the pre-encoded substrate of  claim 1  useful in a method of preparing a labeled synthetic oligomer library comprising a plurality of different members, each member comprising a distinct pre-encoded substrate whereby said one or plurality of pre-encoded substrate is linked to a oligomer structure said method comprising the steps of:
 a) apportioning said pre-encoded substrates among a plurality of reaction vessels;  
 b) exposing said pre-encoded substrates in each reaction vessels to one or a plurality of monomers;  
 c) detecting and recording identifier information for each of said identifier tags in each of said reaction vessels;  
 d) apportioning said pre-encoded substrates among a plurality of reaction vessels; and  
 e) repeating steps a) through c) from at least one to about twenty times wherein said pre-encoded substrate being a pre-encoded microchip.  
 
     
     
       6. One or a plurality of the encodable substrate of  claim 2  useful in a method of preparing a labeled synthetic oligomer library comprising a plurality of different members, each member comprising a distinct encodable substrate whereby said one or plurality of pre-encoded substrate a oligomer structure is linked to a oligomer structure said method comprising the steps of:
 a) apportioning said encodable substrates among a plurality of reaction vessels;  
 b) exposing said encodable substrates in each reaction vessels to one or a plurality of monomers;  
 c) adding first identifier information to said encodable substrates;  
 d) apportioning said encodable substrates among a plurality of reaction vessels; and  
 e) repeating steps a) through c) from at least one to about twenty times wherein said encodable substrate being a encodable microchip.