Interleukin-5 specific recombinant antibodies

Abstract

An effective anti-IL-5 recombinant antibody molecule comprising heavy and/or light chain antigen-binding residues from a donor antibody.

We claim:  
     
       1. A recombinant antibody molecule that specifically binds human Interleukin-5 antigen comprising a composite heavy chain and a composite light chain, said composite heavy chain and light chain forming a variable domain comprising acceptor antibody framework residues, said variable domain comprising corresponding donor residues from murine monoclonal 39D10 heavy chain variable region consisting of the sequence of SEQ ID No:6 at residues 31-35, 50-65 and 95-102 and at framework residues 23, 24, 27-30, 37, 49, 73, 76, 77, and 78, and corresponding donor residues from murine monoclonal antibody 39D10 light chain variable region consisting of the sequence of SEQ ID No:8 at residues 24—34, 50-56 and 89-97 and at framework residues 22, 68, and 71 according to the Kabat numbering system. 
     
     
       2. An antibody molecule according to  claim 1  wherein the acceptor antibody framework residues for the composite heavy chain are human group III heavy chain residues, and the acceptor antibody framework residues for the composite light chain are human group I light chain residues. 
     
     
       3. A recombinant antibody molecule that specifically binds human Interleukin-5 antigen, comprising the a heavy chain variable region amino acid sequence comprising residues  20 - 135  of SEQ ID No: 28, and the a light chain variable region amino acid sequence comprising residues  21 - 128  of SEQ ID No: 26. 
     
     
       4. A composition comprising the antibody molecule of any one of claims  1 ,  2 , or  3  in combination with a pharmaceutically acceptable carrier, diluent or excipient. 
     
     
       5. A recombinant antibody molecule that specifically binds human interleukin- 5  antigen comprising a composite heavy chain and a composite light chain, said composite heavy chain and light chain forming a variable domain comprising acceptor antibody framework residues, said variable domain comprising corresponding donor residues from murine monoclonal antibody  39 D 10  heavy chain variable region consisting of the sequence of SEQ ID NO: 6  at residues  31 - 35 ,  50 - 65  and  95 - 102  and at framework residues  24 ,  27 - 30 ,  37 ,  49 ,  73 ,  76  and  78 , and corresponding donor residues from murine monoclonal antibody  39 D 10  light chain variable region consisting of the sequence of SEQ ID No: 8  at residues  24 - 34 ,  50 - 56  and  89 - 97  and at framework residues  68  and  71  according to the Kabat number system. 
     
     
       6. A recombinant antibody molecule that specifically binds human Interleukin- 5  antigen, comprising a heavy chain variable region encoded by SEQ ID No:  27  and a light chain variable region encoded by SEQ ID No: 25 . 
     
     
       7. A composition comprising the antibody molecule of any one of claims  5  or  6  in combination with a pharmaceutically acceptable carrier, diluent, or excipient.