Manufacture and expression of large structural genes

Abstract

Illustrated is the preparation and expression of manufactured genes capable of directing synthesis of human immune and leukocyte interferons and of other biologically active proteinaceous products, which products differ from naturally-occurring forms in terms of the identity and/or relative position of one or more amino acids, and in terms of one or more biological and pharmacological properties but which substantially retain other such properties.

1. [Met- −1 , des-Cys 1 , des-Tyr 2 , des-Cys 3 ]  A Met −1   , des - Cys   1   , des - Tyr   2   , des - Cys   3    analog polypeptide of human  IFN-γ polypeptide produced  encoded by a DNA sequence coding therefor  in a transformant organism, said polypeptide having substantially the characteristics of human immune interferon.  transformed host cell. 
     
     
       2. A process for producing a Met −1   , des - Cys   1   , des - Tyr   2   , des - Cys   3    analog polypeptide of human IFN - N - γ comprising the steps of    ( a )  growing a host cell transformed with a DNA sequence encoding the analog polypeptide, whereby said host cell expresses said DNA sequences, and      ( b )  isolating the polypeptide produced by step  ( a ) .     
     
     
       3. The process of  claim 2  wherein the host cell is E. coli.