APC antibodies

Abstract

A human gene termed APC is disclosed. Methods and kits are provided for assessing mutations of the APC gene in human tissues and body samples. APC mutations are found in familial adenomatous polyposis patients as well as in sporadic colorectal cancer patients. APC is expressed in most normal tissues. These results suggest that APC is a tumor suppressor.

1. A preparation of antibodies which specifically binds to a human APC (adenomatous polyposis coil) protein having an amino acid sequence as shown in SEQ ID NO:1, 2, or 7, and does not specifically bind to other human proteins. 
     
     
       2. A preparation of antibodies which specifically binds to a human APC protein which is the product of a mutant allele found in a tumor, wherein the antibodies do not specifically bind to other human proteins, and wherein the human APC protein is a mutant form of the amino acid sequence shown in SEQ ID NOS:2 and  SEQ ID NO:  7, and the mutant allele is a mutant form of the nucleotide sequence shown in SEQ ID NO:1  having the sequence of SEQ ID NO: 7 but for a substitution of Arg→Cys at residue 414 . 
     
     
       3. The preparation of  claim 2  wherein the mutant allele contains a mutation selected from the group consisting of mutations at codons 243, 279, 288, 301, 331, 413, 437, 456, 500, 712, and 1338. 
     
     
       4. The preparation of  claim 2  wherein the mutant allele contains a premature stop codon. 
     
     
       5. The preparation of  claim 2  wherein the mutant allele contains a missense mutation. 
     
     
       6. The preparation of  claim 2  wherein the mutant allele contains a frameshift mutation. 
     
     
       7. The preparation of  claim 2  wherein the mutant allele contains a splice junction mutation. 
     
     
       8. The preparation of  claim 2  wherein the mutant allele contains an insertion mutation.