Human immunodeficiency virus (HIV) nucleotide sequences

Abstract

Polynucleotide sequences are provided for the diagnosis of the presence of retroviral infection in a human host associated with lymphadenopathy syndrome and/or acquired immune deficiency syndrome, for expression of polypeptides and use of the polypeptides to prepare antibodies, where both the polypeptides and antibodies may be employed as diagnostic reagents or in therapy, e.g., vaccines and passive immunization. The sequences provide detection of the viral infectious agents associated with the indicated syndromes and can be used for expression of antigenic polypeptides.

1. A single-stranded nucleic acid probe comprising a sequence of at least 20 contiguous bases, wherein the at least 20 bases are fully complementary to at least 20 contiguous bases selected from the gag, pol, or env open reading frame as shown in  FIG. 4  or the complement thereof, said probe not forming a duplex with HTLV-I and -II genomic sequences under conditions of stringency for hybridization under which said probe forms a duplex with either strand of viral DNA from a lambda bacteriophage selected from the group consisting of ATCC Accession number 40143 and 40144. 
     
     
       2. The probe of  claim 1  wherein the open reading frame is the gag open reading frame wherein the gag open reading frame extends from nucleotide 792 to 2298 of FIG.  4 . 
     
     
       3. The probe of  claim 1  wherein the open reading frame is pol open reading frame wherein the pol open reading frame extends from nucleotide 2967 to 5103 of FIG.  4 . 
     
     
       4. The probe of  claim 1  wherein the probe comprises RNA. 
     
     
       5. The probe of  claim 1  wherein the probe comprises DNA. 
     
     
       6. The probe of  claim 2  wherein the probe comprises RNA. 
     
     
       7. The probe of  claim 2  wherein the probe comprises DNA. 
     
     
       8. The probe of  claim 3  wherein the probe comprises RNA. 
     
     
       9. The probe of  claim 3  wherein the probe comprises DNA. 
     
     
       10. The probe of  claim 1  wherein the open reading frame is the env open reading frame, wherein the env open reading frame extends from nucleotide 6235 to 8799 of FIG.  4 . 
     
     
       11. The probe of  claim 10  wherein the probe comprises RNA. 
     
     
       12. The probe of  claim 10  wherein the probe comprises DNA. 
     
     
       13. The probe of  claim 1  further comprising a label. 
     
     
       14. The probe of  claim 2  further comprising a label. 
     
     
       15. The probe of  claim 3  further comprising a label. 
     
     
       16. The probe of  claim 4  further comprising a label. 
     
     
       17. The probe of  claim 5  further comprising a label. 
     
     
       18. The probe of  claim 6  further comprising a label. 
     
     
       19. The probe of  claim 7  further comprising a label. 
     
     
       20. The probe of  claim 8  further comprising a label. 
     
     
       21. The probe of  claim 9  further comprising a label. 
     
     
       22. The probe of  claim 10  further comprising a label. 
     
     
       23. The probe of  claim 11  further comprising a label. 
     
     
       24. The probe of  claim 12  further comprising a label.