Purification, composition and specificity of Heparinase I, II, and III from Flavobacterium heparinum

Abstract

A single, reproducible scheme to simultaneously purify all three of the heparin lyases from F. heparinum to apparent homogeneity is disclosed herein. The kinetic properties of the heparin lyases have been determined as well as the conditions to optimize their activity and stability. Mono-clonal antibodies to the three heparinases are also described and are useful for detection, isolation and characterization of the heparinases.

1. A method for cleaving hexosamine-glucuronic acid linkages in linear polysacharides  polysaccharides of D-glucosamine linked to hexuronic acid comprising reacting heparin or heparan sulfate with a  purified heparinase selected from the group consisting of heparinase II present in   Flavobacterium heparinum  heparinase II free of lyase activity other than heparinase II activity, having a molecular weight of 84,100, cleaving heparin and heparan sulfate and having a pH optimum  pI of 8.9-9.1 and heparinase III which is expressed in   Flavobacterium heparinum  heparinase III free of lyase activity other than heparinase III activity, having a molecular weight of 70,800, cleaving heparan sulfate, and having a pH optimum  pI of 9.9-10.1. 
     
     
       2. The method of  claim 1  wherein the heparin is in extracellular matrix of cells or tissues. 
     
     
       3. The method of  claim 1  wherein the heparinase II has a pH optimum of  6 . 9  on heparan sulfate and the heparinase III has a pH optimum of  7 . 6  on heparan sulfate.