Compositions and methods for enhancing receptor-mediated cellular internalization

Abstract

Compositions and methods for improving cellular internalization of one or more compounds are disclosed. The compositions include a compound to be delivered and a biocompatible viscous material, such as a hydrogel, lipogel, or highly viscous sol. The composition also include, or are administered in conjunction with, an enhancer in an amount effective to maximize expression of or binding to receptors and enhance RME of the compound into the cells. This leads to high transport rates of compounds to be delivered across cell membranes, facilitating more efficient delivery of drugs and diagnostic agents. Compositions are applied topically orally, nasally, vaginally, rectally, and ocularly. The enhancer is administered with the composition or separately, either systemically or preferably locally. The compound to be delivered can also be the enhancer.

1. A method for delivering an agent to cells at a site where uptake is desired comprising administering to the cells at the site where uptake is desired a composition comprising
 (a) a viscous material having an apparent viscosity between 10 and 2000 Poise and approximately the same apparent viscosity, at a shear stress of between approximately 1 and 200 Pascal at a strain rate approximately that of endocytosis, as the cytosolic fluid of the cell to which the agent is to be delivered,    (b) a compound to be delivered, and    (c) an enhancer in an amount effective to enhance expression of or binding to receptors eliciting receptor-mediated endocytosis on the cells at the site where uptake is desired.    
     
     
       2. The  A method of  claim 1   for delivering an agent to cells at a site where uptake is desired comprising administering to the cells at the site where uptake is desired a composition comprising
 ( a )  a viscous material having an apparent viscosity between  10  and  200  Poise and approximately the same apparent viscosity, at a shear stress of between approximately  1  and  200  Pascal at a strain rate approximately that of endocytosis, as the cytosolic fluid of the cell to which the agent is to be delivered,    
 ( b )  a compound to be delivered, and    
 ( c )  an enhancer in an amount effective to enhance expression of or binding to receptors eliciting receptor - mediated endocytosis on the cells at the site where uptake is desired,  wherein the enhancer is a steroid  selected from the group consisting of steroids and hormones, and wherein the enhancer is different from the compound to be delivered.  
 
     
     
       3. The method of claim  1    2  wherein the cells to which the compound is to be delivered are in the nose, rectum, mouth, ear, eye, or lungs. 
     
     
       4. The method of claim  1    2  wherein the composition is administered topically. 
     
     
       5. The method of claim  1    2  wherein the enhancer is administered systemically. 
     
     
       6. The method of claim  1    2  wherein the composition is administered to the vaginal mucosa. 
     
     
       7. The method of claim  1    2  wherein the compound to be delivered is selected from the group consisting of proteins, peptides, carbohydrates, nuceilc  nucleic acid molecules, and chemotherapeutic agents. 
     
     
       8. The method of  claim 7  wherein the enhancer is selected from the group consisting of hormones, glucocorticoids, and other molecules specifically binding to a receptor on a cell surface to induce endocytosis. 
     
     
       9. The method of claim  8    2  wherein the enhancer is a reproductive hormone. 
     
     
       10. The method of claim  1    2  wherein the enhancer is a glucocorticoid. 
     
     
       11. The method of  claim 9  wherein the reproductive hormone is selected from the group consisting of progesterone, estradiol, and combinations thereof. 
     
     
       12. The method of claim  1    2  wherein the viscous material is selected from the group consisting of hydrogels, lipogels and sols. 
     
     
       13. The method of  claim 12  wherein the hydrogel is selected from the group consisting of celluloses, polyalkyleneoxide, polyvinylpyrrolidone, dextrans, alginates, agaroses, gelatin, hyaluronic acid, trehalose, polyvinyl alcohol, and copolymers and blends thereof. 
     
     
       14. A composition for delivering an agent to cells at a site where uptake is desired comprising:
 (a) a viscous fluid having an apparent viscosity between 10 and 2000 Poise and approximately the same apparent viscosity, at a shear stress of between approximately 1 and 200 Pascal at a strain rate approximately that of endocytosis, as the cytosolic fluid of the cell to which the agent is to be delivered,    (b) a compound to be delivered, and    (c) an enhancer in an amount effective to enhance expression of or binding to receptors eliciting receptor-mediated endocytosis on the cells at the site where uptake is desired.    
     
     
       15. The composition of claim  14    17  in a formulation suitable for administration to mucosa of tissue selected from group consisting of the nose, the rectum, the vagina, the mouth, the ear, the eye, and the lungs. 
     
     
       16. The composition of claim  14    17  wherein the compound to be delivered is selected from the group consisting of proteins, peptides, carbohydrates, nucleic acid molecules, and chemotherapeutic agents. 
     
     
       17. The  A composition of  claim 14   for delivering an agent to cells at a site where uptake is desired comprising:
 ( a )  a viscous fluid having an apparent viscosity between  10  and  200  Poise and approximately the same apparent viscosity, at a shear stress of between approximately  1  and  200  Pascal at a strain rate approximately that of endocytosis, as the cytosolic fluid of the cell to which the agent is to be delivered,    
 ( b )  a compound to be delivered, and    
 ( c )  an enhancer in an amount effective to enhance expression of or binding to receptors eliciting receptor - mediated endocytosis on the cells at the site where uptake is desired,  wherein the enhancer is selected from the group consisting of hormones and glucocorticoids  steroids, and wherein the enhancer is different from the compound to be delivered.  
 
     
     
       18. The composition of claim  14    17  wherein the hormone is a reproductive hormone. 
     
     
       19. The composition of claim  14    17  wherein the viscous material is selected from the group consisting of hydrogels, lipogels and sols. 
     
     
       20. The composition of  claim 19  wherein the hydrogel is selected from the group consisting of celluloses, polyalkyleneoxide, polyvinylpyrrolidone, dextrans, alginates, agaroses, gelatin, hyaluronic acid, trehalose, polyvinyl alcohol, and copolymers and blends thereof. 
     
     
       21. A kit for delivering a compound to cells comprising:
 a first composition comprising a viscous fluid and the compound to be delivered, wherein the viscous fluid has an apparent viscosity between 10 and 2000  200 Poise and approximately the same apparent viscosity, at a shear stress of between approximately 1 and 200 Pascal at a strain rate approximately that of endocytosis, as the cytosolic fluid of the cell to which the agent is to be delivered, and  
 a second composition comprising an enhancer selected from the group consisting of hormones and steroids in an amount effective to enhance expression of receptors eliciting receptor-mediated endocytosis on the cells, thereby to enhance receptor mediated endocytosis of the agent into the cells,  
   wherein the enhancer is different from the compound to be delivered .  
 
     
     
       22. The kit of  claim 21  wherein the second composition is in a formulation suitable for topical or systemic administration.