Low insertion force tip/mandrel

Abstract

A pipette assembly for automatic pipetting machines including a stepped mandrel and tip for providing a minimum contact seal between the pipette tip and mandrel. The mandrel includes a first cylindrical portion with a first exterior diameter and a second cylindrical portion with a second exterior diameter. Raised bands on the first and second cylindrical portions contact the interior wall of the pipette tip to form seals. Because only a portion of the mandrel, specifically the seal portions, contacts the pipette tip, lower forces are required to insert the tip onto the mandrel.

1. A pipette mandrel for engagement with the interior wall of a pipette tip, the pipette mandrel comprising:
 a. an elongated hollow structure, the hollow structure including a proximate end and a distal end; 
 b. a lead-in portion formed on the distal end of the hollow structure; 
 c. a first cylindrical portion adjacent to the lead-in portion, the first cylindrical portion having a first exterior diameter,;  
 d. a second cylindrical portion adjacent to the first cylindrical portion, the second cylindrical portion having a second exterior diameter that is greater than the first exterior diameter; 
 e. a first non-resilient raised band positioned upon the first cylindrical portion, the first raised band having a diameter greater that than the first exterior diameter of the first cylindrical portion, the first raised band for contacting the interior wall of the pipette tip to form a first seal between the first raised band and the pipette tip; and 
 f. a second raised band positioned upon the second cylindrical portion, the second raised band having a diameter greater that than the second exterior diameter of the second cylindrical portion, the second raised band for contacting the interior wall of the pipette tip to form a second seal between the second raised band and the pipette tip. 
 
     
     
       2. The pipette mandrel of  claim 1  wherein the first raised band is adjacent to the lead-in portion such that the lead-in portion tapers into the first raised band. 
     
     
       3. The pipette mandrel of  claim 1  further comprising a taper between the first and second cylindrical portion. 
     
     
       4. The pipette mandrel of  claim 3 , wherein the second raised band is positioned on the end of the second cylindrical portion substantially adjacent to the taper. 
     
     
       5. A pipette assembly comprising:
 a. a pipette tip including a collar portion and an adjacent head, the head for receiving or expelling fluids from the tip, the collar portion including an interior cylindrical wall having a first interior diameter and a second interior diameter separated by a step portion, the interior cylindrical wall of the pipette tip further comprising a positive stop and the interior cylindrical wall void of any raised sealing rings; and  
 b. a hollow mandrel having a proximate end and a distal end, the mandrel comprising:
 (1) a lead-in portion formed on the distal end of the mandrel; 
 (2) a first cylindrical portion adjacent to the lean-in lead-in portion, the first cylindrical portion having a first exterior diameter; 
 (3) a first non-resilient raised band positioned upon the first cylindrical portion, the first raised band having a diameter greater that than the first exterior diameter of the first cylindrical portion, the first raised band contacting the interior cylindrical wall of the collar portion to form a first seal between the mandrel and the pipette tip first non-resilient raised band and the interior cylindrical wall; and 
 (4) a second cylindrical portion adjacent to the first cylindrical portion, the second cylindrical portion having a second exterior diameter. 
 
 
     
     
       6. The pipette assembly of  claim 5  wherein a flange between the collar portion and conical the head is the positive stop. 
     
     
       7. The pipette assembly of  claim 5  A pipette assembly comprising:
 a. a pipette tip including a collar portion and an adjacent head, the head for receiving or expelling fluids from the tip, the collar portion including an interior cylindrical wall having a first interior diameter and a second interior diameter separated by a step portion, the interior cylindrical wall of the pipette tip further comprising a positive stop, wherein a puller ring on the interior wall of the pipette tip is the positive stop, and the interior cylindrical wall void of any raised sealing rings; and 
 b. a hollow mandrel having a proximate end and a distal end, the mandrel comprising: (1) a lead-in portion formed on the distal end of the mandrel; (2) a first cylindrical portion adjacent to the lead-in portion, the first cylindrical portion having a first exterior diameter; (3) a first non-resilient raised band positioned upon the first cylindrical portion, the first raised band having a diameter greater than the first exterior diameter of the first cylindrical portion, the first raised band contacting the interior cylindrical wall of the collar portion to form a first seal between the mandrel and the pipette tip; and (4) a second cylindrical portion adjacent to the first cylindrical portion, the second cylindrical portion having a second exterior diameter. 
 
     
     
       8. The pipette assembly of  claim 7  wherein the collar portion of the pipette tip further comprises vertical ribs positioned on the exterior of the collar portion of the pipette tip. 
     
     
       9. The pipette assembly of  claim 5  wherein the mandrel further includes a second cylindrical portion adjacent to the first cylindrical portion, the second cylindrical portion having a second exterior diameter collar portion comprises a mouth comprising a funnel-shaped taper. 
     
     
       10. The pipette assembly of the  claim 9  wherein the mandrel further includes a second raised band positioned upon the second cylindrical portion, the second raised band having a diameter greater than the second exterior diameter of the second: cylindrical portion. 
     
     
       11. The pipette assembly of  claim 10  A pipette assembly comprising:
 a. a pipette tip including a collar portion and an adjacent head, the head for receiving or expelling fluids from the tip, the collar portion including an interior cylindrical wall having a first interior diameter and a second interior diameter separated by a step portion, the interior cylindrical wall of the pipette tip further comprising a positive stop and the interior cylindrical wall void of any raised sealing rings, wherein the collar portion comprises a mouth comprising a funnel -shaped taper; and 
 b. a hollow mandrel having a proximate end and a distal end, the mandrel comprising: (1) a lead-in portion formed on the distal end of the mandrel; (2) a first cylindrical portion adjacent to the lead-in portion, the first cylindrical portion having a first exterior diameter; (3) a first raised band positioned upon the first cylindrical portion, the first raised band having a diameter greater than the first exterior diameter of the first cylindrical portion, the first raised band contacting the interior cylindrical wall of the collar portion to form a first seal between the mandrel and the pipette tip; and (4) a second cylindrical portion adjacent to the first cylindrical portion, the second cylindrical portion having a second exterior diameter, wherein the mandrel further includes a second raised band positioned upon the second cylindrical portion, the second raised band having a diameter greater than the second exterior diameter of the second cylindrical portion, wherein the second raised band contacts the interior cylindrical wall of the collar portion to form a second seal between the mandrel and the pipette tip. 
 
     
     
       12. A method for connecting a pipette mandrel to a pipette tip comprising:
 a. providing a pipette tip comprising a collar portion and an adjacent conical head, the conical head for receiving or expelling fluids from the tip, the collar portion including an interior cylindrical wall having a first interior diameter; 
 b. providing a hollow mandrel having a proximate end and a distal end, the mandrel comprising:
 (1) a lead-in portion formed on the distal end of the mandrel; 
 (2) a first cylindrical portion adjacent to the lead-in portion, the first cylindrical portion having a first exterior diameter, 
 (3) a first non-resilient raised band portioned positioned upon the first cylindrical portion, the first raised band having a diameter greater than the first exterior diameter; and 
 (4) a second cylindrical portion having a second exterior diameter that is greater than the first exterior diameter; and 
 (5) a second raised band positioned upon the second cylindrical portion, the second raised band having a diameter greater that than the second exterior diameter of the second cylindrical portion; and  
 
 c. inserting said pipette tip onto said mandrel such that the first non-resilient raised band contacts the interior cylindrical wall of the collar portion to form a first seal between the mandrel and the pipette tip and the second raised band contacting the cylindrical wall of the collar portion to form a second seal between the mandrel and the pipette tip. 
 
     
     
       13. The method of  claim 12  comprising the step of removing the pipette tip from the mandrel such that the first raised band disengages the interior cylindrical wall of the collar portion. 
     
     
       14. A pipette tip and mandrel assembly comprising:
 a. a mandrel comprising
 (1) a first cylindrical portion having a first exterior diameter, the first cylindrical portion having a first non-resilient raised band positioned upon the first cylindrical portion; 
 (2) a second cylindrical portion connected to the first cylindrical portion, the first cylindrical portion having a second exterior diameter different than the first exterior diameter; and 
   b. a pipette tip positioned upon the mandrel, the pipette tip comprising
 (1) a head for receiving and expelling liquids; 
 (2) a collar connected to the head, the collar designed and adapted to fit over the first cylindrical portion and the second cylindrical portion of the mandrel, the collar comprising an interior cylindrical wall having a first interior diameter portion and a second interior diameter portion such that the diameter of the second interior diameter portion is different than the diameter of the first interior diameter portion; wherein the first interior diameter portion of the collar engages the first non-resilient raised band of the mandrel to form a first seal, and the second interior diameter portion of the collar fits over the second cylindrical portion of the mandrel. 
   
     
     
       15. The pipette and mandrel assembly of  claim 14  wherein the mandrel further comprises a second non-resilient raised band positioned upon the second cylindrical portion. 
     
     
       16. The pipette and mandrel assembly of  claim 15  A pipette tip and mandrel assembly comprising:
 a. a mandrel comprising (1) a first cylindrical portion having a first exterior diameter, the first cylindrical portion having a first non-resilient raised band positioned upon the first cylindrical portion; (2) a second cylindrical portion comprising a second non-resilient raised band positioned upon the second cylindrical portion connected to the first cylindrical portion, the first cylindrical portion having a second exterior diameter different than the first exterior diameter; and 
 b. a pipette tip positioned upon the mandrel, the pipette tip comprising (1) a head for receiving and expelling liquids; (2) a collar connected to the head, the collar designed and adapted to fit over the first cylindrical portion and the second cylindrical portion of the mandrel, the collar comprising an interior cylindrical wall having a first interior diameter portion and a second interior diameter portion such that the diameter of the second interior diameter portion is different than the diameter of the first interior diameter portion; wherein the first interior diameter portion of the collar engages the first non-resilient raised band of the mandrel to form a first seal, and the second interior diameter portion of the collar fits over the second cylindrical portion of the mandrel, wherein the second interior diameter portion of the collar engages the second non-resilient raised band of the mandrel to form a second seal. 
 
     
     
       17. The pipette and mandrel assembly of  claim 14  A pipette tip and mandrel assembly comprising:
 a. a mandrel comprising (1) a first cylindrical portion having a first exterior diameter, the first cylindrical portion having a first non-resilient raised band positioned upon the first cylindrical portion; (2) a second cylindrical portion connected to the first cylindrical portion, the second cylindrical portion having a second exterior diameter different than the first exterior diameter; and 
 b. a pipette tip positioned upon the mandrel, the pipette tip comprising (1) a head for receiving and expelling liquids; (2) a collar connected to the head, the collar designed and adapted to fit over the first cylindrical portion and the second cylindrical portion of the mandrel, the collar comprising an interior cylindrical wall having a first interior diameter portion and a second interior diameter portion such that the diameter of the second interior diameter portion is different than the diameter of the first interior diameter portion; wherein the first interior diameter portion of the collar engages the first non-resilient raised band of the mandrel to form a first seal between the first non-resilient raised band of the mandrel and the first interior diameter portion of the collar, wherein the second interior diameter portion of the collar fits over the second cylindrical portion of the mandrel, wherein the interior cylindrical wall of the pipette tip is void of any raised sealing rings. 
 
     
     
       18. The pipette assembly of  claim 14  wherein the interior cylindrical wall of the pipette tip further comprises a positive stop. 
     
     
       19. A pipette tip for use with a mandrel, the pipette tip comprising
 a head for receiving and expelling liquids; and   a collar connected to the head and comprising a first internal cylindrical surface region that is divided by a molded internal ring, the molded internal ring serving as a positive stop for the mandrel when the mandrel is inserted into the pipette tip, wherein the diameter of the first cylindrical surface region is substantially the same on either side of the molded internal ring immediately adjacent to either side of the molded internal ring.    
     
     
       20. The pipette tip of claim 19 wherein the collar comprises a second internal cylindrical surface region such that the diameter of the second internal cylindrical surface region is different than the diameter of the first internal cylindrical surface region.  
     
     
       21. The pipette tip of claim 20 wherein the mandrel comprises a first cylindrical portion and a second cylindrical portion connected to the first cylindrical portion, wherein the first cylindrical portion comprises a first non-resilient raised band, and wherein the first interior diameter portion of the collar is adapted to engage the first non-resilient raised band of the mandrel to form a first seal, and the second interior diameter portion of the collar is adapted to fit over the second cylindrical portion of the mandrel.  
     
     
       22. The pipette tip of claim 21 wherein the first and second internal surface regions are void of any raised sealing rings.  
     
     
       23. The pipette tip of claim 20 further comprising a tapered portion between the first internal cylindrical surface region and the second internal cylindrical surface region.  
     
     
       24. The pipette tip of claim 22 wherein the head comprises a conical shape.  
     
     
       25. The pipette tip of claim 20 wherein the first and second internal surface regions are void of any raised sealing rings.  
     
     
       26. The pipette tip of claim 19 wherein the pipette tip comprises polypropylene.  
     
     
       27. The pipette tip of claim 19 wherein the collar comprises a mouth defined by a rim and a funnel-shaped taper.  
     
     
       28. The pipette tip of claim 19 wherein the head is conically shaped.  
     
     
       29. The pipette tip of claim 19 wherein the collar further comprises a plurality of axial ribs.  
     
     
       30. The pipette tip of claim 20 wherein the mandrel comprises a first sealing feature and a second sealing feature, wherein the first interior diameter portion of the collar is adapted to engage the first sealing feature of the mandrel to form a first seal, and the second interior portion of the collar is adapted to engage the second sealing feature of the mandrel to form a second seal.  
     
     
       31. A pipette tip for use with a mandrel, the pipette tip comprising
 a head for receiving and expelling liquids, wherein the head further comprises a plurality of puller rings formed in an interior of the head; and   a collar connected to the head and comprising a molded internal ring, which serves as a positive stop for the mandrel when the mandrel is inserted into the pipette tip.    
     
     
       32. A pipette tip configured to engage a mandrel comprising a first cylindrical portion having a first exterior diameter, the first cylindrical portion having a first non-resilient raised band positioned upon the first cylindrical portion; and a second cylindrical portion connected to the first cylindrical portion, the second cylindrical portion having a second exterior diameter different than the first exterior diameter, wherein the pipette tip comprises:
 a head for receiving and expelling liquids; and   a collar connected to the head, the collar designed and adapted to fit over the first cylindrical portion and the second cylindrical portion of the mandrel, the collar comprising an interior cylindrical wall having a first interior diameter portion and a second interior diameter portion such that the diameter of the second interior diameter portion is different than the diameter of the first interior diameter portion,   wherein the first interior diameter portion of the collar is configured to engage the first non-resilient raised band of the mandrel to form a first seal between the first interior diameter portion of the interior cylindrical wall of the collar and the first non-resilient raised band of the mandrel, and the second interior diameter portion of the collar is configured to fit over the second cylindrical portion of the mandrel, wherein the interior cylindrical wall of the pipette tip is void of any raised sealing rings.    
     
     
       33. The pipette tip of claim 32 wherein the interior cylindrical wall of the pipette tip further comprises a positive stop.  
     
     
       34. A pipette tip configured to engage a mandrel comprising a first cylindrical portion having a first exterior diameter, the first cylindrical portion having a first non-resilient raised band positioned upon the first cylindrical portion; and a second cylindrical portion connected to the first cylindrical portion, the second cylindrical portion having a second exterior diameter different than the first exterior diameter, wherein the pipette tip comprises:
 a head for receiving and expelling liquids; and   a collar connected to the head, the collar designed and adapted to fit over the first cylindrical portion and the second cylindrical portion of the mandrel, the collar comprising an interior cylindrical wall having a first interior diameter portion and a second interior diameter portion such that the diameter of the second interior diameter portion is different than the diameter of the first interior diameter portion, wherein the interior cylindrical wall of the pipette tip is void of any raised sealing rings, wherein the interior cylindrical wall of the pipette tip further comprises a positive stop, wherein the positive stop is in the form of a molded internal ring,   wherein the first interior diameter portion of the collar is configured to engage the first non-resilient raised band of the mandrel to form a first seal, and the second interior diameter portion of the collar is configured to fit over the second cylindrical portion of the mandrel.    
     
     
       35. A method for connecting a pipette mandrel to a pipette tip, the pipette tip comprising a collar portion and an adjacent conical head, the conical head for receiving or expelling fluids from the tip, the collar portion including an interior cylindrical wall having a first interior diameter, and the mandrel having a proximate end and a distal end, the mandrel comprising: (1) a lead-in portion formed on the distal end of the mandrel; (2) a first cylindrical portion adjacent to the lead-in portion, the first cylindrical portion having a first exterior diameter, (3) a first non-resilient raised band positioned upon the first cylindrical portion, the first raised band having a diameter greater than the first exterior diameter; and (4) a second cylindrical portion having a second exterior diameter that is greater than the first exterior diameter; and (5) a second raised band positioned upon the second cylindrical portion, the second raised band having a diameter greater that the second exterior diameter of the second cylindrical portion, the method comprising:
 inserting said pipette tip onto said mandrel such that the first non-resilient raised band contacts the interior cylindrical wall of the collar portion to form a first seal between the mandrel and the pipette tip and the second raised band contacting the cylindrical wall of the collar portion to form a second seal between the mandrel and the pipette tip.    
     
     
       36. The method of claim 35 further comprising the step of removing the pipette tip from the mandrel such that the first raised band disengages the interior cylindrical wall of the collar portion.