P
USRE43586EExpiredUtilityPatentIndex 76

Antibodies to prostate-specific membrane antigen

Assignee: ISRAELI RON SPriority: Nov 5, 1992Filed: Sep 12, 2008Granted: Aug 14, 2012
Est. expiryNov 5, 2012(expired)· nominal 20-yr term from priority
Inventors:ISRAELI RON SHESTON WARREN D WFAIR WILLIAM RFAIR LEGAL REPRESENTATIVE MARYANN
G01N 2800/342C07K 16/3069C07K 14/4748C07K 14/705
76
PatentIndex Score
12
Cited by
558
References
33
Claims

Abstract

This invention provides purified antibodies to the outer membrane domain of prostate-specific membrane (PSM) antigen, compositions of matter comprising PSM antigen antibodies conjugated to a radioisotope or a toxin, and a method of imaging prostate cancer by using PSM antigen antibodies.

Claims

exact text as granted — not AI-modified
1. A purified monoclonal antibody which binds to a fragment of an outer membrane domain of prostate specific membrane antigen, which fragment has within its structure the consecutive amino acid sequence Asp-Glu-Leu-Lys-Ala-Glu (SEQ ID NO: 35). 
     
     
       2. A purified monoclonal antibody which binds to a fragment of an outer membrane domain of prostate specific membrane antigen, which fragment has within its structure the consecutive amino acid sequence Asn-Glu-Asp-Gly-Asn-Glu (SEQ ID NO: 36). 
     
     
       3. A purified monoclonal antibody which binds to a fragment of an outer membrane domain of prostate specific membrane antigen, which fragment has within its structure the consecutive amino acid sequence Lys-Ser-Pro-Asp-Glu-Gly (SEQ ID NO: 37). 
     
     
       4. A purified monoclonal antibody which binds to a fragment of an outer membrane domain of prostate specific membrane antigen, which fragment has within its structure each of the following amino acid sequences:
 (a) Asp-Glu-Leu-Lys-Ala-Glu (SEQ ID NO: 35);   (b) Asn-Glu-Asp-Gly-Asn-Glu (SEQ ID NO: 36);   (c) Lys-Ser-Pro-Asp-Glu-Gly (SEQ ID NO: 37); and   (d) Ala-Gly-Ala-Leu-Val-Leu-Ala-Gly-Gly-Phe-Phe-Leu-Leu-Gly-Phe-Leu-Phe (SEQ ID 80:38).   
     
     
       5. A purified monoclonal antibody which binds to a fragment of an outer membrane domain of prostate specific membrane antigen, which fragment has within its structure each of the following amino acid sequences:
 (a) Asp-Glu-Leu-Lys-Ala-Glu (SEQ ID NO: 35); 
 (b) Asn-Glu-Asp-Gly-Asn-Glu (SEQ ID NO: 36); and 
 (c) Lys-Ser-Pro-Asp-Glu-Gly (SEQ ID NO: 37). 
 
     
     
       6. A purified monoclonal antibody which binds to a fragment of prostate specific membrane antigen, which fragment corresponds to a hydrophilic region of an outer membrane domain of prostate specific membrane antigen, the amino acid sequence of which antigen is set forth in SEQ ID NO:2. 
     
     
       7. A purified monoclonal antibody which binds to a hydrophilic region of an outer membrane domain of prostate specific membrane antigen, which hydrophilic region has within its structure the consecutive amino acid sequence Asp-Glu-Leu-Lys-Ala-Glu (SEQ ID NO: 35). 
     
     
       8. A purified monoclonal antibody which binds to a hydrophilic region of an outer membrane domain of prostate specific membrane antigen, which hydrophilic region has within its structure the consecutive amino acid sequence Asn-Glu-Asp-Gly-Asn-Glu (SEQ ID NO: 36). 
     
     
       9. A purified monoclonal antibody which binds to a hydrophilic region of an outer membrane domain of prostate specific membrane antigen, which hydrophilic region has within its structure the consecutive amino acid sequence Lys-Ser-Pro-Asp-Glu-Gly (SEQ ID NO: 37). 
     
     
       10. A purified monoclonal antibody which binds to an outer membrane domain of prostate specific membrane antigen, the amino acid sequence of which antigen is set forth in SEQ ID NO:2. 
     
     
       11. A purified monoclonal antibody which binds to a hydrophilic region of an outer membrane domain of prostate specific membrane antigen, the amino acid sequence of which antigen is set forth in SEQ ID NO:2. 
     
     
       12. The purified antibody of any one of  claims 1 - 11 , wherein the antibody is a monoclonal antibody. 
     
     
       13. A composition of matter comprising the a monoclonal antibody of any one of  1 - 11  and an agent conjugated to the monoclonal antibody thereto, wherein the monoclonal antibody binds to an outer membrane domain of prostate-specific membrane antigen, the amino acid sequence of which antigen is set forth in SEQ ID NO:2. 
     
     
       14. The composition of matter of  claim 13 , wherein the agent is a radioisotope or toxin. 
     
     
       15. A composition comprising a carrier and the composition of matter of  claim 13 . 
     
     
       16. A method of imaging prostate cancer in a subject which comprises administering to the subject the composition of matter of  claim 13 , wherein the agent is an imaging agent under conditions permitting formation of a complex between the composition of matter and prostate specific membrane antigen, and obtaining an image of any complex so formed. 
     
     
       17. A monoclonal antibody having an antigen-binding region -specific for the extracellular domain of prostate specific membrane antigen, the amino acid sequence of which antigen is set forth in SEQ ID NO:2. 
     
     
       18. A composition of matter comprising a monoclonal antibody and an agent conjugated thereto, wherein the monoclonal antibody binds to a fragment of an outer membrane domain of prostate-specific membrane antigen, which fragment has within its structure the consecutive amino acid sequence Asp-Glu-Leu-Lys-Ala-Glu (SEQ ID NO:35). 
     
     
       19. A composition of matter comprising a monoclonal antibody and an agent conjugated thereto, wherein the monoclonal antibody binds to a fragment of an outer membrane domain of prostate-specific membrane antigen, which fragment has within its structure the consecutive amino acid sequence Asn-Glu-Asp-Gly-Asn-Glu (SEQ ID NO:36). 
     
     
       20. A composition of matter comprising a monoclonal antibody and an agent conjugated thereto, wherein the monoclonal antibody binds to a fragment of an outer membrane domain of prostate-specific membrane antigen, which fragment has within its structure the consecutive amino acid sequence Lys-Ser-Pro-Asp-Glu-Gly (SEQ ID NO:37). 
     
     
       21. A composition of matter comprising a monoclonal antibody and an agent conjugated thereto, wherein the monoclonal antibody binds to a fragment of an outer membrane domain of prostate-specific membrane antigen, which fragment has within its structure each of the following amino acid sequences: (a) Asp-Glu-Leu-Lys-Ala-Glu (SEQ ID NO: 35); (b) Asn-Glu-Asp-Gly-Asn-Glu (SEQ ID NO: 36); and (c) Lys-Ser-Pro-Asp-Glu-Gly (SEQ ID NO: 37). 
     
     
       22. A composition of matter comprising a monoclonal antibody and an agent conjugated thereto, wherein the monoclonal antibody binds to a fragment of prostate-specific membrane antigen, which fragment corresponds to a hydrophilic region of an outer membrane domain of prostate-specific membrane antigen, the amino acid sequence of which antigen is set forth in SEQ ID NO:2. 
     
     
       23. A composition of matter comprising a monoclonal antibody and an agent conjugated thereto, wherein the monoclonal antibody binds to a hydrophilic region of an outer membrane domain of prostate-specific membrane antigen, which hydrophilic region has within its structure the consecutive amino acid sequence Asp-Glu-Leu-Lys-Ala-Glu (SEQ ID NO:35). 
     
     
       24. A composition of matter comprising a monoclonal antibody and an agent conjugated thereto, wherein the monoclonal antibody binds to a hydrophilic region of an outer membrane domain of prostate-specific membrane antigen, which hydrophilic region has within its structure the consecutive amino acid sequence Asn-Glu-Asp-Gly-Asn-Glu (SEQ ID NO:36). 
     
     
       25. A composition of comprising a monoclonal antibody and an agent conjugated thereto, wherein the monoclonal antibody binds to a hydrophilic region of an outer membrane domain of prostate-specific membrane antigen, which hydrophilic region has within its structure the consecutive amino acid sequence Lys-Ser-Pro-Asp-Glu-Gly (SEQ ID NO:37). 
     
     
       26. A composition of matter comprising a monoclonal antibody and an agent conjugated thereto, wherein the monoclonal antibody binds to a hydrophilic region of an outer membrane domain of prostate specific membrane antigen, the amino acid sequence of which antigen is set forth in SEQ ID NO:2. 
     
     
       27. The composition of matter of any of claim 18-20, or 21-26, wherein the agent is a radioisotope or toxin. 
     
     
       28. A composition comprising a carrier and the composition of matter of any of claim 18-20 or 21-26. 
     
     
       29. A method of imaging prostate cancer in a subject which comprises administering to the subject the composition of matter of any of claim 18-20 or 21-26, wherein the agent is an imaging agent, under conditions permitting formation of a complex between the composition of matter and prostate-specific membrane antigen, and obtaining an image of any complex so formed. 
     
     
       30. A composition comprising a carrier and the composition of matter of claim 27. 
     
     
       31. The composition of claim 30, wherein the agent is a radioisotope. 
     
     
       32. The composition of claim 30, wherein the agent is a toxin. 
     
     
       33. The method of claim 29, wherein the imaging agent is a radioisotope.

Cited by (0)

No later patents cite this yet.

References (0)

No backward citations on record.