USRE44359EExpiredUtilityPatentIndex 59

Nucleic acid molecules encoding anti-P-selectin antibodies

Assignee: GRAUS YVOPriority: Apr 13, 2004Filed: May 15, 2012Granted: Jul 9, 2013

Est. expiryApr 13, 2024(expired)· nominal 20-yr term from priority

Inventors:GRAUS YVO HIMBER JACQUES JANSEN-MOLENAAR MIRANDA KLING DOROTHEE KOPETZKI ERHARD PARREN PAUL REBERS FRANK STEINER BEAT STERN ANNE BRAEMER PAMELA STUBENRAUCH KAY-GUNNAR VAN DE WINKEL JAN VAN VUGT MARTINE

A61P 9/10A61P 7/02A61P 29/00C07K 2317/565C07K 2317/92C07K 2317/21C07K 2317/56C07K 16/2854C07K 2317/52Y10S435/81C07K 2317/76C07K 16/46C07K 16/28C12N 15/09

PatentIndex Score

Cited by

References

169

Claims

Abstract

This invention relates to anti-P-selectin antibodies and, in particular, to anti-P-selectin antibodies and variants thereof that contain an Fc part derived from human origin and do not bind complement factor C1q. These antibodies have new and inventive properties causing a benefit for a patient suffering from critical limb ischemia or peripheral arterial occlusive disease (CLI/PAOD).

Claims

exact text as granted — not AI-modified

The invention claimed is:

1. A nucleic acid molecule encoding a humanized or human antibody that binds to P-selectin selected from the group consisting of:
(a) a nucleic acid molecule encoding an antibody wherein the light chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 43 for CDR1, SEQ ID NO: 45 for CDR2, and SEQ ID NO: 47 for CDR3; and the heavy chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 29 for CDR1, SEQ ID NO: 33 for CDR2, and SEQ ID NO: 38 for CDR3;
(b) a nucleic acid molecule encoding an antibody wherein the light chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 43 for CDR1, SEQ ID NO: 45 for CDR2, and SEQ ID NO: 48 for CDR3; and the heavy chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 30 for CDR1, SEQ ID NO: 34 for CDR2, and SEQ ID NO: 39 for CDR3;
(c) a nucleic acid molecule encoding an antibody wherein the light chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 44 for CDR1, SEQ ID NO: 46 for CDR2, and SEQ ID NO: 49 for CDR3; and the heavy chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 31 for CDR1, SEQ ID NO: 35 for CDR2, and SEQ ID NO: 40 for CDR3;
(d) a nucleic acid molecule encoding an antibody wherein the light chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 44 for CDR1, SEQ ID NO: 46 for CDR2, and SEQ ID NO: 49 for CDR3; and the heavy chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 32 for CDR1, SEQ ID NO: 36 for CDR2, and SEQ ID NO: 41 for CDR3;
(e) a nucleic acid molecule encoding an antibody wherein the light chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 43 for CDR1, SEQ ID NO: 45 for CDR2, and SEQ ID NO: 50 for CDR3; and the heavy chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 32 for CDR1, SEQ ID NO: 36 for CDR2, and SEQ ID NO: 41 for CDR3;
(f) a nucleic acid molecule encoding an antibody wherein the light chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 43 for CDR1, SEQ ID NO: 45 for CDR2, and SEQ ID NO: 50 for CDR3; and the heavy chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 31 for CDR1, SEQ ID NO: 35 for CDR2, and SEQ ID NO: 40 for CDR3;
(g) a nucleic acid molecule encoding an antibody wherein the light chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 44 for CDR1, SEQ ID NO: 46 for CDR2, and SEQ ID NO: 51 for CDR3; and the heavy chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 29 for CDR1, SEQ ID NO: 37 for CDR2, and SEQ ID NO: 42 for CDR3;
(h) a nucleic acid molecule encoding an antibody wherein the light chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 44 for CDR1, SEQ ID NO: 46 for CDR2, and SEQ ID NO: 51 for CDR3; and the heavy chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 29 for CDR1, SEQ ID NO: 37 for CDR2, and SEQ ID NO: 42 for CDR3; and
(i) a nucleic acid molecule encoding an antibody wherein the light chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 43 for CDR1, SEQ ID NO: 45 for CDR2, and SEQ ID NO: 52 for CDR3; and the heavy chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 29 for CDR1, SEQ ID NO: 37 for CDR2, and SEQ ID NO: 42 for CDR3.

2. A nucleic acid molecule of claim 1 encoding an antibody comprising a heavy chain constant region having an amino acid sequence of SEQ ID NO: 25, SEQ ID NO: 26, or SEQ ID NO: 28.

3. A nucleic acid molecule of claim 1 encoding an antibody comprising a light chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 43 for CDR1, SEQ ID NO: 45 for CDR2, and SEQ ID NO: 48 for CDR3; and a heavy chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 30 for CDR1, SEQ ID NO: 34 for CDR2, and SEQ ID NO: 39 for CDR3.

4. A nucleic acid molecule of claim 3 encoding an antibody comprising a heavy chain constant region having the amino acid sequence of SEQ ID NO: 28.

5. A nucleic acid molecule of claim 3 encoding an antibody comprising a light chain constant region having the amino acid sequence of SEQ ID NO: 23.

6. A nucleic acid molecule of claim 3 encoding an antibody comprising a heavy chain constant region having the amino acid sequence of SEQ ID NO: 28 and a light chain constant region having the amino acid sequence of SEQ ID NO: 23.

7. A nucleic acid molecule of claim 1 selected from the group consisting of:
(a) a nucleic acid molecule encoding an antibody wherein the light chain variable region has the amino acid sequence of SEQ ID NO: 1 and the heavy chain variable region has the amino acid sequence of SEQ ID NO: 2;
(b) a nucleic acid molecule encoding an antibody wherein the light chain variable region has the amino acid sequence of SEQ ID NO: 3 and the heavy chain variable region has the amino acid sequence of SEQ ID NO: 4;
(c) a nucleic acid molecule encoding an antibody wherein the light chain variable region has the amino acid sequence of SEQ ID NO: 5 and the heavy chain variable region has the amino acid sequence of SEQ ID NO: 6;
(d) a nucleic acid molecule encoding an antibody wherein the light chain variable region has the amino acid sequence of SEQ ID NO: 7 and the heavy chain variable region has the amino acid sequence of SEQ ID NO: 8;
(e) a nucleic acid molecule encoding an antibody wherein the light chain variable region has the amino acid sequence of SEQ ID NO: 9 and the heavy chain variable region has the amino acid sequence of SEQ ID NO: 10;
(f) a nucleic acid molecule encoding an antibody wherein the light chain variable region has the amino acid sequence of SEQ ID NO: 11 and the heavy chain variable region has the amino acid sequence of SEQ ID NO: 12;
(g) a nucleic acid molecule encoding an antibody wherein the light chain variable region has the amino acid sequence of SEQ ID NO: 13 and the heavy chain variable region has the amino acid sequence of SEQ ID NO: 14;
(h) a nucleic acid molecule encoding an antibody wherein the light chain variable region has the amino acid sequence of SEQ ID NO: 15 and the heavy chain variable region has the amino acid sequence of SEQ ID NO: 16; and
(i) a nucleic acid molecule encoding an antibody wherein the light chain variable region has the amino acid sequence of SEQ ID NO: 17 and the heavy chain variable region has the amino acid sequence of SEQ ID NO: 18.

8. A nucleic acid molecule of claim 7 encoding an antibody comprising a heavy chain constant region having an amino acid sequence of SEQ ID NO: 25, SEQ ID NO: 26, or SEQ ID NO: 28.

9. A nucleic acid molecule of claim 7 encoding an antibody comprising a light chain constant region having the amino acid sequence of SEQ ID NO: 23.

10. A nucleic acid molecule of claim 7 encoding an antibody comprising a heavy chain constant region having an amino acid sequence of SEQ ID NO: 25, SEQ ID NO: 26, or SEQ ID NO: 28; and a light chain constant region having the amino acid sequence of SEQ ID NO: 23.

11. A nucleic acid molecule of claim 7 encoding an antibody comprising a light chain variable region having the amino acid sequence of SEQ ID NO: 3 and a heavy chain variable region having the amino acid sequence of SEQ ID NO: 4.

12. A nucleic acid molecule of claim 11 encoding an antibody comprising a heavy chain constant region having the amino acid sequence of SEQ ID NO: 28.

13. A nucleic acid molecule of claim 11 encoding an antibody comprising a light chain constant region having the amino acid sequence of SEQ ID NO: 23.

14. A nucleic acid molecule of claim 11 encoding an antibody comprising a heavy chain constant region having the amino acid sequence of SEQ ID NO: 28 and a light chain constant region having the amino acid sequence of SEQ ID NO: 23.

15. A nucleic acid molecule encoding a humanized or human antibody that binds to P-selectin wherein the antibody comprises a light chain variable region having an amino acid sequence selected from the group consisting of: SEQ ID NO: 1; SEQ ID NO: 3; SEQ ID NO: 5; SEQ ID NO: 7; SEQ ID NO: 9; SEQ ID NO: 11; SEQ ID NO: 13; SEQ ID NO: 15; SEQ ID NO: 17; SEQ ID NO: 19; and SEQ ID NO: 21; or a heavy chain variable region having an amino acid sequence selected from the group consisting of: SEQ ID NO: 2; SEQ ID NO: 4; SEQ ID NO: 6; SEQ ID NO: 8; SEQ ID NO: 10; SEQ ID NO: 12; SEQ ID NO: 14; SEQ ID NO: 16; SEQ ID NO: 18; SEQ ID NO: 20; and SEQ ID NO: 22.

16. A nucleic acid molecule of claim 15 encoding an antibody comprising a light chain variable region having the amino acid sequence of SEQ ID NO: 3.

17. A nucleic acid molecule of claim 15 encoding an antibody comprising a heavy chain variable region having the amino acid sequence of SEQ ID NO: 4.

18. A nucleic acid molecule encoding a humanized or human antibody that binds to P-selectin selected from the group consisting of:
(a) a nucleic acid molecule encoding an antibody wherein the light chain variable region of the antibody comprises the amino acid sequences of the 3 complementarity determining regions in SEQ ID NO: 19; and the heavy chain variable region of the antibody comprises the amino acid sequences of the 3 complementarity determining regions in SEQ ID NO: 20; and
(b) a nucleic acid molecule encoding an antibody wherein the light chain variable region of the antibody comprises the amino acid sequences of the 3 complementarity determining regions in SEQ ID NO: 21; and the heavy chain variable region of the antibody comprises the amino acid sequences of the 3 complementarity determining regions in SEQ ID NO: 22.

19. A nucleic acid molecule of claim 18 encoding an antibody comprising a light chain variable region comprising the 3 complementarity determining regions in SEQ ID NO: 19; and a heavy chain variable region comprising the 3 complementarity determining regions in SEQ ID NO: 20.

20. A nucleic acid molecule of claim 18 encoding an antibody comprising a light chain variable region comprising the 3 complementarity determining regions in SEQ ID NO: 21; and a heavy chain variable region comprising the 3 complementarity determining regions in SEQ ID NO: 22.

21. A nucleic acid molecule of claim 1 encoding an antibody comprising a light chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 43 for CDR1, SEQ ID NO: 45 for CDR2, and SEQ ID NO: 47 for CDR3; and a heavy chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 29 for CDR1, SEQ ID NO: 33 for CDR2, and SEQ ID NO: 38 for CDR3.

22. A nucleic acid molecule of claim 1 encoding an antibody comprising a light chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 44 for CDR1, SEQ ID NO: 46 for CDR2, and SEQ ID NO: 49 for CDR3; and a heavy chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 31 for CDR1, SEQ ID NO: 35 for CDR2, and SEQ ID NO: 40 for CDR3.

23. A nucleic acid molecule of claim 1 encoding an antibody comprising a light chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 44 for CDR1, SEQ ID NO: 46 for CDR2, and SEQ ID NO: 49 for CDR3; and a heavy chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 32 for CDR1, SEQ ID NO: 36 for CDR2, and SEQ ID NO: 41 for CDR3.

24. A nucleic acid molecule of claim 1 encoding an antibody comprising a light chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 43 for CDR1, SEQ ID NO: 45 for CDR2, and SEQ ID NO: 50 for CDR3; and a heavy chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 32 for CDR1, SEQ ID NO: 36 for CDR2, and SEQ ID NO: 41 for CDR3.

25. A nucleic acid molecule of claim 1 encoding an antibody comprising a light chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 43 for CDR1, SEQ ID NO: 45 for CDR2, and SEQ ID NO: 50 for CDR3; and a heavy chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 31 for CDR1, SEQ ID NO: 35 for CDR2, and SEQ ID NO: 40 for CDR3.

26. A nucleic acid molecule of claim 1 encoding an antibody comprising a light chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 44 for CDR1, SEQ ID NO: 46 for CDR2, and SEQ ID NO: 51 for CDR3; and a heavy chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 29 for CDR1, SEQ ID NO: 37 for CDR2, and SEQ ID NO: 42 for CDR3.

27. A nucleic acid molecule of claim 1 encoding an antibody comprising a light chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 44 for CDR1, SEQ ID NO: 46 for CDR2, and SEQ ID NO: 51 for CDR3; and a heavy chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 29 for CDR1, SEQ ID NO: 37 for CDR2, and SEQ ID NO: 42 for CDR3.

28. A nucleic acid molecule of claim 1 encoding an antibody comprising a light chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 43 for CDR1, SEQ ID NO: 45 for CDR2, and SEQ ID NO: 52 for CDR3; and a heavy chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 29 for CDR1, SEQ ID NO: 37 for CDR2, and SEQ ID NO: 42 for CDR3.

29. A vector comprising a nucleic acid molecule of claim 1 .

30. A host cell comprising a vector of claim 29 .

31. A vector comprising a nucleic acid molecule of claim 3 .

32. A host cell comprising a vector of claim 31 .

33. A vector comprising a nucleic acid molecule of claim 4 .

34. A host cell comprising a vector of claim 33 .

35. A vector comprising a nucleic acid molecule of claim 11 .

36. A host cell comprising a vector of claim 35 .

37. A vector comprising a nucleic acid molecule of claim 14 .

38. A host cell comprising a vector of claim 37 .

39. A vector comprising a nucleic acid molecule of claim 16 .

40. A host cell comprising a vector of claim 39 .

41. A vector comprising a nucleic acid molecule of claim 17 .

42. A host cell comprising a vector of claim 41 .

43. A nucleic acid molecule encoding a humanized antibody that binds to P-selectin selected from the group consisting of:
(a) a nucleic acid molecule encoding an antibody wherein the light chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 43 for CDR1, SEQ ID NO: 45 for CDR2, and SEQ ID NO: 47 for CDR3; and the heavy chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 29 for CDR1, SEQ ID NO: 33 for CDR2, and SEQ ID NO: 38 for CDR3; (b) a nucleic acid molecule encoding an antibody wherein the light chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 43 for CDR1, SEQ ID NO: 45 for CDR2, and SEQ ID NO: 48 for CDR3; and the heavy chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 30 for CDR1, SEQ ID NO: 34 for CDR2, and SEQ ID NO: 39 for CDR3; (c) a nucleic acid molecule encoding an antibody wherein the light chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 44 for CDR1, SEQ ID NO: 46 for CDR2, and SEQ ID NO: 49 for CDR3; and the heavy chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 31 for CDR1, SEQ ID NO: 35 for CDR2, and SEQ ID NO: 40 for CDR3; (d) a nucleic acid molecule encoding an antibody wherein the light chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 44 for CDR1, SEQ ID NO: 46 for CDR2, and SEQ ID NO: 49 for CDR3; and the heavy chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 32 for CDR1, SEQ ID NO: 36 for CDR2, and SEQ ID NO: 41 for CDR3; (e) a nucleic acid molecule encoding an antibody wherein the light chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 43 for CDR1, SEQ ID NO: 45 for CDR2, and SEQ ID NO: 50 for CDR3; and the heavy chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 32 for CDR1, SEQ ID NO: 36 for CDR2, and SEQ ID NO: 41 for CDR3; (f) a nucleic acid molecule encoding an antibody wherein the light chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 43 for CDR1, SEQ ID NO: 45 for CDR2, and SEQ ID NO: 50 for CDR3; and the heavy chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 31 for CDR1, SEQ ID NO: 35 for CDR2, and SEQ ID NO: 40 for CDR3; (g) a nucleic acid molecule encoding an antibody wherein the light chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 44 for CDR1, SEQ ID NO: 46 for CDR2, and SEQ ID NO: 51 for CDR3; and the heavy chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 29 for CDR1, SEQ ID NO: 37 for CDR2, and SEQ ID NO: 42 for CDR3; (h) a nucleic acid molecule encoding an antibody wherein the light chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 44 for CDR1, SEQ ID NO: 46 for CDR2, and SEQ ID NO: 51 for CDR3; and the heavy chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 29 for CDR1, SEQ ID NO: 37 for CDR2, and SEQ ID NO: 42 for CDR3; and (i) a nucleic acid molecule encoding an antibody wherein the light chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 43 for CDR1, SEQ ID NO: 45 for CDR2, and SEQ ID NO: 52 for CDR3; and the heavy chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 29 for CDR1, SEQ ID NO: 37 for CDR2, and SEQ ID NO: 42 for CDR3.

44. A nucleic acid molecule of claim 43 encoding an antibody comprising a heavy chain constant region having an amino acid sequence of SEQ ID NO: 25, SEQ ID NO: 26, or SEQ ID NO: 28.

45. A nucleic acid molecule of claim 43 encoding an antibody comprising a light chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 43 for CDR1, SEQ ID NO: 45 for CDR2, and SEQ ID NO: 48 for CDR3; and a heavy chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 30 for CDR1, SEQ ID NO: 34 for CDR2, and SEQ ID NO: 39 for CDR3.

46. A nucleic acid molecule of claim 45 encoding an antibody comprising a heavy chain constant region having the amino acid sequence of SEQ ID NO: 28.

47. A nucleic acid molecule of claim 45 encoding an antibody comprising a light chain constant region having the amino acid sequence of SEQ ID NO: 23.

48. A nucleic acid molecule of claim 45 encoding an antibody comprising a heavy chain constant region having the amino acid sequence of SEQ ID NO: 28 and a light chain constant region having the amino acid sequence of SEQ ID NO: 23.

49. A nucleic acid molecule of claim 43 selected from the group consisting of:
(a) a nucleic acid molecule encoding an antibody wherein the light chain variable region has the amino acid sequence of SEQ ID NO: 1 and the heavy chain variable region has the amino acid sequence of SEQ ID NO: 2; (b) a nucleic acid molecule encoding an antibody wherein the light chain variable region has the amino acid sequence of SEQ ID NO: 3 and the heavy chain variable region has the amino acid sequence of SEQ ID NO: 4; (c) a nucleic acid molecule encoding an antibody wherein the light chain variable region has the amino acid sequence of SEQ ID NO: 5 and the heavy chain variable region has the amino acid sequence of SEQ ID NO: 6; (d) a nucleic acid molecule encoding an antibody wherein the light chain variable region has the amino acid sequence of SEQ ID NO: 7 and the heavy chain variable region has the amino acid sequence of SEQ ID NO: 8; (e) a nucleic acid molecule encoding an antibody wherein the light chain variable region has the amino acid sequence of SEQ ID NO: 9 and the heavy chain variable region has the amino acid sequence of SEQ ID NO: 10; (f) a nucleic acid molecule encoding an antibody wherein the light chain variable region has the amino acid sequence of SEQ ID NO: 11 and the heavy chain variable region has the amino acid sequence of SEQ ID NO: 12; (g) a nucleic acid molecule encoding an antibody wherein the light chain variable region has the amino acid sequence of SEQ ID NO: 13 and the heavy chain variable region has the amino acid sequence of SEQ ID NO: 14; (h) a nucleic acid molecule encoding an antibody wherein the light chain variable region has the amino acid sequence of SEQ ID NO: 15 and the heavy chain variable region has the amino acid sequence of SEQ ID NO: 16; and (i) a nucleic acid molecule encoding an antibody wherein the light chain variable region has the amino acid sequence of SEQ ID NO: 17 and the heavy chain variable region has the amino acid sequence of SEQ ID NO: 18.

50. A nucleic acid molecule of claim 49 encoding an antibody comprising a heavy chain constant region having an amino acid sequence of SEQ ID NO: 25, SEQ ID NO: 26, or SEQ ID NO: 28.

51. A nucleic acid molecule of claim 49 encoding an antibody comprising a light chain constant region having the amino acid sequence of SEQ ID NO: 23.

52. A nucleic acid molecule of claim 49 encoding an antibody comprising a heavy chain constant region having an amino acid sequence of SEQ ID NO: 25, SEQ ID NO: 26, or SEQ ID NO: 28; and a light chain constant region having the amino acid sequence of SEQ ID NO: 23.

53. A nucleic acid molecule of claim 49 encoding an antibody comprising a light chain variable region having the amino acid sequence of SEQ ID NO: 3 and a heavy chain variable region having the amino acid sequence of SEQ ID NO: 4.

54. A nucleic acid molecule of claim 53 encoding an antibody comprising a heavy chain constant region having the amino acid sequence of SEQ ID NO: 28.

55. A nucleic acid molecule of claim 53 encoding an antibody comprising a light chain constant region having the amino acid sequence of SEQ ID NO: 23.

56. A nucleic acid molecule of claim 53 encoding an antibody comprising a heavy chain constant region having the amino acid sequence of SEQ ID NO: 28 and a light chain constant region having the amino acid sequence of SEQ ID NO: 23.

57. A nucleic acid molecule encoding a humanized antibody that binds to P-selectin wherein the antibody comprises a light chain variable region having an amino acid sequence selected from the group consisting of: SEQ ID NO: 1; SEQ ID NO: 3; SEQ ID NO: 5; SEQ ID NO: 7; SEQ ID NO: 9; SEQ ID NO: 11; SEQ ID NO: 13; SEQ ID NO: 15; SEQ ID NO: 17; SEQ ID NO: 19; and SEQ ID NO: 21; or a heavy chain variable region having an amino acid sequence selected from the group consisting of: SEQ ID NO: 2; SEQ ID NO: 4; SEQ ID NO: 6; SEQ ID NO: 8; SEQ ID NO: 10; SEQ ID NO: 12; SEQ ID NO: 14; SEQ ID NO: 16; SEQ ID NO: 18; SEQ ID NO: 20; and SEQ ID NO: 22.

58. A nucleic acid molecule of claim 57 encoding an antibody comprising a light chain variable region having the amino acid sequence of SEQ ID NO: 3.

59. A nucleic acid molecule of claim 57 encoding an antibody comprising a heavy chain variable region having the amino acid sequence of SEQ ID NO: 4.

60. A nucleic acid molecule encoding a humanized antibody that binds to P-selectin selected from the group consisting of:
(a) a nucleic acid molecule encoding an antibody wherein the light chain variable region of the antibody comprises the amino acid sequences of the 3 complementarity determining regions in SEQ ID NO: 19; and the heavy chain variable region of the antibody comprises the amino acid sequences of the 3 complementarity determining regions in SEQ ID NO: 20; and (b) a nucleic acid molecule encoding an antibody wherein the light chain variable region of the antibody comprises the amino acid sequences of the 3 complementarity determining regions in SEQ ID NO: 21; and the heavy chain variable region of the antibody comprises the amino acid sequences of the 3 complementarity determining regions in SEQ ID NO: 22.

61. A nucleic acid molecule of claim 60 encoding an antibody comprising a light chain variable region comprising the 3 complementarity determining regions in SEQ ID NO: 19; and a heavy chain variable region comprising the 3 complementarity determining regions in SEQ ID NO: 20.

62. A nucleic acid molecule of claim 60 encoding an antibody comprising a light chain variable region comprising the 3 complementarity determining regions in SEQ ID NO: 21; and a heavy chain variable region comprising the 3 complementarity determining regions in SEQ ID NO: 22.

63. A nucleic acid molecule of claim 43 encoding an antibody comprising a light chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 43 for CDR1, SEQ ID NO: 45 for CDR2, and SEQ ID NO: 47 for CDR3; and a heavy chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 29 for CDR1, SEQ ID NO: 33 for CDR2, and SEQ ID NO: 38 for CDR3.

64. A nucleic acid molecule of claim 43 encoding an antibody comprising a light chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 44 for CDR1, SEQ ID NO: 46 for CDR2, and SEQ ID NO: 49 for CDR3; and a heavy chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 31 for CDR1, SEQ ID NO: 35 for CDR2, and SEQ ID NO: 40 for CDR3.

65. A nucleic acid molecule of claim 43 encoding an antibody comprising a light chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 44 for CDR1, SEQ ID NO: 46 for CDR2, and SEQ ID NO: 49 for CDR3; and a heavy chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 32 for CDR1, SEQ ID NO: 36 for CDR2, and SEQ ID NO: 41 for CDR3.

66. A nucleic acid molecule of claim 43 encoding an antibody comprising a light chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 43 for CDR1, SEQ ID NO: 45 for CDR2, and SEQ ID NO: 50 for CDR3; and a heavy chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 32 for CDR1, SEQ ID NO: 36 for CDR2, and SEQ ID NO: 41 for CDR3.

67. A nucleic acid molecule of claim 43 encoding an antibody comprising a light chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 43 for CDR1, SEQ ID NO: 45 for CDR2, and SEQ ID NO: 50 for CDR3; and a heavy chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 31 for CDR1, SEQ ID NO: 35 for CDR2, and SEQ ID NO: 40 for CDR3.

68. A nucleic acid molecule of claim 43 encoding an antibody comprising a light chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 44 for CDR1, SEQ ID NO: 46 for CDR2, and SEQ ID NO: 51 for CDR3; and a heavy chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 29 for CDR1, SEQ ID NO: 37 for CDR2, and SEQ ID NO: 42 for CDR3.

69. A nucleic acid molecule of claim 43 encoding an antibody comprising a light chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 44 for CDR1, SEQ ID NO: 46 for CDR2, and SEQ ID NO: 51 for CDR3; and a heavy chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 29 for CDR1, SEQ ID NO: 37 for CDR2, and SEQ ID NO: 42 for CDR3.

70. A nucleic acid molecule of claim 43 encoding an antibody comprising a light chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 43 for CDR1, SEQ ID NO: 45 for CDR2, and SEQ ID NO: 52 for CDR3; and a heavy chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 29 for CDR1, SEQ ID NO: 37 for CDR2, and SEQ ID NO: 42 for CDR3.

71. A vector comprising a nucleic acid molecule of claim 43.

72. A host cell comprising a vector of claim 71.

73. A vector comprising a nucleic acid molecule of claim 45.

74. A host cell comprising a vector of claim 73.

75. A vector comprising a nucleic acid molecule of claim 46.

76. A host cell comprising a vector of claim 75.

77. A vector comprising a nucleic acid molecule of claim 53.

78. A host cell comprising a vector of claim 77.

79. A vector comprising a nucleic acid molecule of claim 56.

80. A host cell comprising a vector of claim 79.

81. A vector comprising a nucleic acid molecule of claim 58.

82. A host cell comprising a vector of claim 81.

83. A vector comprising a nucleic acid molecule of claim 59.

84. A host cell comprising a vector of claim 83.

85. A nucleic acid molecule encoding a human antibody that binds to P-selectin selected from the group consisting of:
(a) a nucleic acid molecule encoding an antibody wherein the light chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 43 for CDR1, SEQ ID NO: 45 for CDR2, and SEQ ID NO: 47 for CDR3; and the heavy chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 29 for CDR1, SEQ ID NO: 33 for CDR2, and SEQ ID NO: 38 for CDR3; (b) a nucleic acid molecule encoding an antibody wherein the light chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 43 for CDR1, SEQ ID NO: 45 for CDR2, and SEQ ID NO: 48 for CDR3; and the heavy chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 30 for CDR1, SEQ ID NO: 34 for CDR2, and SEQ ID NO: 39 for CDR3; (c) a nucleic acid molecule encoding an antibody wherein the light chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 44 for CDR1, SEQ ID NO: 46 for CDR2, and SEQ ID NO: 49 for CDR3; and the heavy chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 31 for CDR1, SEQ ID NO: 35 for CDR2, and SEQ ID NO: 40 for CDR3; (d) a nucleic acid molecule encoding an antibody wherein the light chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 44 for CDR1, SEQ ID NO: 46 for CDR2, and SEQ ID NO: 49 for CDR3; and the heavy chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 32 for CDR1, SEQ ID NO: 36 for CDR2, and SEQ ID NO: 41 for CDR3; (e) a nucleic acid molecule encoding an antibody wherein the light chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 43 for CDR1, SEQ ID NO: 45 for CDR2, and SEQ ID NO: 50 for CDR3; and the heavy chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 32 for CDR1, SEQ ID NO: 36 for CDR2, and SEQ ID NO: 41 for CDR3; (f) a nucleic acid molecule encoding an antibody wherein the light chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 43 for CDR1, SEQ ID NO: 45 for CDR2, and SEQ ID NO: 50 for CDR3; and the heavy chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 31 for CDR1, SEQ ID NO: 35 for CDR2, and SEQ ID NO: 40 for CDR3; (g) a nucleic acid molecule encoding an antibody wherein the light chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 44 for CDR1, SEQ ID NO: 46 for CDR2, and SEQ ID NO: 51 for CDR3; and the heavy chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 29 for CDR1, SEQ ID NO: 37 for CDR2, and SEQ ID NO: 42 for CDR3; (h) a nucleic acid molecule encoding an antibody wherein the light chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 44 for CDR1, SEQ ID NO: 46 for CDR2, and SEQ ID NO: 51 for CDR3; and the heavy chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 29 for CDR1, SEQ ID NO: 37 for CDR2, and SEQ ID NO: 42 for CDR3; and (i) a nucleic acid molecule encoding an antibody wherein the light chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 43 for CDR1, SEQ ID NO: 45 for CDR2, and SEQ ID NO: 52 for CDR3; and the heavy chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 29 for CDR1, SEQ ID NO: 37 for CDR2, and SEQ ID NO: 42 for CDR3.

86. A nucleic acid molecule of claim 85 encoding an antibody comprising a heavy chain constant region having an amino acid sequence of SEQ ID NO: 25, SEQ ID NO: 26, or SEQ ID NO: 28.

87. A nucleic acid molecule of claim 85 encoding an antibody comprising a light chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 43 for CDR1, SEQ ID NO: 45 for CDR2, and SEQ ID NO: 48 for CDR3; and a heavy chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 30 for CDR1, SEQ ID NO: 34 for CDR2, and SEQ ID NO: 39 for CDR3.

88. A nucleic acid molecule of claim 87 encoding an antibody comprising a heavy chain constant region having the amino acid sequence of SEQ ID NO: 28.

89. A nucleic acid molecule of claim 87 encoding an antibody comprising a light chain constant region having the amino acid sequence of SEQ ID NO: 23.

90. A nucleic acid molecule of claim 87 encoding an antibody comprising a heavy chain constant region having the amino acid sequence of SEQ ID NO: 28 and a light chain constant region having the amino acid sequence of SEQ ID NO: 23.

91. A nucleic acid molecule of claim 85 selected from the group consisting of:
(a) a nucleic acid molecule encoding an antibody wherein the light chain variable region has the amino acid sequence of SEQ ID NO: 1 and the heavy chain variable region has the amino acid sequence of SEQ ID NO: 2; (b) a nucleic acid molecule encoding an antibody wherein the light chain variable region has the amino acid sequence of SEQ ID NO: 3 and the heavy chain variable region has the amino acid sequence of SEQ ID NO: 4; (c) a nucleic acid molecule encoding an antibody wherein the light chain variable region has the amino acid sequence of SEQ ID NO: 5 and the heavy chain variable region has the amino acid sequence of SEQ ID NO: 6; (d) a nucleic acid molecule encoding an antibody wherein the light chain variable region has the amino acid sequence of SEQ ID NO: 7 and the heavy chain variable region has the amino acid sequence of SEQ ID NO: 8; (e) a nucleic acid molecule encoding an antibody wherein the light chain variable region has the amino acid sequence of SEQ ID NO: 9 and the heavy chain variable region has the amino acid sequence of SEQ ID NO: 10; (f) a nucleic acid molecule encoding an antibody wherein the light chain variable region has the amino acid sequence of SEQ ID NO: 11 and the heavy chain variable region has the amino acid sequence of SEQ ID NO: 12; (g) a nucleic acid molecule encoding an antibody wherein the light chain variable region has the amino acid sequence of SEQ ID NO: 13 and the heavy chain variable region has the amino acid sequence of SEQ ID NO: 14; (h) a nucleic acid molecule encoding an antibody wherein the light chain variable region has the amino acid sequence of SEQ ID NO: 15 and the heavy chain variable region has the amino acid sequence of SEQ ID NO: 16; and (i) a nucleic acid molecule encoding an antibody wherein the light chain variable region has the amino acid sequence of SEQ ID NO: 17 and the heavy chain variable region has the amino acid sequence of SEQ ID NO: 18.

92. A nucleic acid molecule of claim 91 encoding an antibody comprising a heavy chain constant region having an amino acid sequence of SEQ ID NO: 25, SEQ ID NO: 26, or SEQ ID NO: 28.

93. A nucleic acid molecule of claim 91 encoding an antibody comprising a light chain constant region having the amino acid sequence of SEQ ID NO: 23.

94. A nucleic acid molecule of claim 91 encoding an antibody comprising a heavy chain constant region having an amino acid sequence of SEQ ID NO: 25, SEQ ID NO: 26, or SEQ ID NO: 28; and a light chain constant region having the amino acid sequence of SEQ ID NO: 23.

95. A nucleic acid molecule of claim 91 encoding an antibody comprising a light chain variable region having the amino acid sequence of SEQ ID NO: 3 and a heavy chain variable region having the amino acid sequence of SEQ ID NO: 4.

96. A nucleic acid molecule of claim 95 encoding an antibody comprising a heavy chain constant region having the amino acid sequence of SEQ ID NO: 28.

97. A nucleic acid molecule of claim 95 encoding an antibody comprising a light chain constant region having the amino acid sequence of SEQ ID NO: 23.

98. A nucleic acid molecule of claim 95 encoding an antibody comprising a heavy chain constant region having the amino acid sequence of SEQ ID NO: 28 and a light chain constant region having the amino acid sequence of SEQ ID NO: 23.

99. A nucleic acid molecule encoding a human antibody that binds to P-selectin wherein the antibody comprises a light chain variable region having an amino acid sequence selected from the group consisting of: SEQ ID NO: 1; SEQ ID NO: 3; SEQ ID NO: 5; SEQ ID NO: 7; SEQ ID NO: 9; SEQ ID NO: 11; SEQ ID NO: 13; SEQ ID NO: 15; SEQ ID NO: 17; SEQ ID NO: 19; and SEQ ID NO: 21; or a heavy chain variable region having an amino acid sequence selected from the group consisting of: SEQ ID NO: 2; SEQ ID NO: 4; SEQ ID NO: 6; SEQ ID NO: 8; SEQ ID NO: 10; SEQ ID NO: 12; SEQ ID NO: 14; SEQ ID NO: 16; SEQ ID NO: 18; SEQ ID NO: 20; and SEQ ID NO: 22.

100. A nucleic acid molecule of claim 99 encoding an antibody comprising a light chain variable region having the amino acid sequence of SEQ ID NO: 3.

101. A nucleic acid molecule of claim 99 encoding an antibody comprising a heavy chain variable region having the amino acid sequence of SEQ ID NO: 4.

102. A nucleic acid molecule encoding a human antibody that binds to P-selectin selected from the group consisting of:
(a) a nucleic acid molecule encoding an antibody wherein the light chain variable region of the antibody comprises the amino acid sequences of the 3 complementarity determining regions in SEQ ID NO: 19; and the heavy chain variable region of the antibody comprises the amino acid sequences of the 3 complementarity determining regions in SEQ ID NO: 20; and (b) a nucleic acid molecule encoding an antibody wherein the light chain variable region of the antibody comprises the amino acid sequences of the 3 complementarity determining regions in SEQ ID NO: 21; and the heavy chain variable region of the antibody comprises the amino acid sequences of the 3 complementarity determining regions in SEQ ID NO: 22.

103. A nucleic acid molecule of claim 102 encoding an antibody comprising a light chain variable region comprising the 3 complementarity determining regions in SEQ ID NO: 19; and a heavy chain variable region comprising the 3 complementarity determining regions in SEQ ID NO: 20.

104. A nucleic acid molecule of claim 102 encoding an antibody comprising a light chain variable region comprising the 3 complementarity determining regions in SEQ ID NO: 21; and a heavy chain variable region comprising the 3 complementarity determining regions in SEQ ID NO: 22.

105. A nucleic acid molecule of claim 85 encoding an antibody comprising a light chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 43 for CDR1, SEQ ID NO: 45 for CDR2, and SEQ ID NO: 47 for CDR3; and a heavy chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 29 for CDR1, SEQ ID NO: 33 for CDR2, and SEQ ID NO: 38 for CDR3.

106. A nucleic acid molecule of claim 85 encoding an antibody comprising a light chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 44 for CDR1, SEQ ID NO: 46 for CDR2, and SEQ ID NO: 49 for CDR3; and a heavy chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 31 for CDR1, SEQ ID NO: 35 for CDR2, and SEQ ID NO: 40 for CDR3.

107. A nucleic acid molecule of claim 85 encoding an antibody comprising a light chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 44 for CDR1, SEQ ID NO: 46 for CDR2, and SEQ ID NO: 49 for CDR3; and a heavy chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 32 for CDR1, SEQ ID NO: 36 for CDR2, and SEQ ID NO: 41 for CDR3.

108. A nucleic acid molecule of claim 85 encoding an antibody comprising a light chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 43 for CDR1, SEQ ID NO: 45 for CDR2, and SEQ ID NO: 50 for CDR3; and a heavy chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 32 for CDR1, SEQ ID NO: 36 for CDR2, and SEQ ID NO: 41 for CDR3.

109. A nucleic acid molecule of claim 85 encoding an antibody comprising a light chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 43 for CDR1, SEQ ID NO: 45 for CDR2, and SEQ ID NO: 50 for CDR3; and a heavy chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 31 for CDR1, SEQ ID NO: 35 for CDR2, and SEQ ID NO: 40 for CDR3.

110. A nucleic acid molecule of claim 85 encoding an antibody comprising a light chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 44 for CDR1, SEQ ID NO: 46 for CDR2, and SEQ ID NO: 51 for CDR3; and a heavy chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 29 for CDR1, SEQ ID NO: 37 for CDR2, and SEQ ID NO: 42 for CDR3.

111. A nucleic acid molecule of claim 85 encoding an antibody comprising a light chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 44 for CDR1, SEQ ID NO: 46 for CDR2, and SEQ ID NO: 51 for CDR3; and a heavy chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 29 for CDR1, SEQ ID NO: 37 for CDR2, and SEQ ID NO: 42 for CDR3.

112. A nucleic acid molecule of claim 85 encoding an antibody comprising a light chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 43 for CDR1, SEQ ID NO: 45 for CDR2, and SEQ ID NO: 52 for CDR3; and a heavy chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 29 for CDR1, SEQ ID NO: 37 for CDR2, and SEQ ID NO: 42 for CDR3.

113. A vector comprising a nucleic acid molecule of claim 85.

114. A host cell comprising a vector of claim 113.

115. A vector comprising a nucleic acid molecule of claim 87.

116. A host cell comprising a vector of claim 115.

117. A vector comprising a nucleic acid molecule of claim 88.

118. A host cell comprising a vector of claim 117.

119. A vector comprising a nucleic acid molecule of claim 95.

120. A host cell comprising a vector of claim 119.

121. A vector comprising a nucleic acid molecule of claim 98.

122. A host cell comprising a vector of claim 121.

123. A vector comprising a nucleic acid molecule of claim 100.

124. A host cell comprising a vector of claim 123.

125. A vector comprising a nucleic acid molecule of claim 101.

126. A host cell comprising a vector of claim 125.

127. A nucleic acid molecule encoding an antibody that binds to P-selectin selected from the group consisting of:
(a) a nucleic acid molecule encoding an antibody wherein the light chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 43 for CDR1, SEQ ID NO: 45 for CDR2, and SEQ ID NO: 47 for CDR3; and the heavy chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 29 for CDR1, SEQ ID NO: 33 for CDR2, and SEQ ID NO: 38 for CDR3; (b) a nucleic acid molecule encoding an antibody wherein the light chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 43 for CDR1, SEQ ID NO: 45 for CDR2, and SEQ ID NO: 48 for CDR3; and the heavy chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 30 for CDR1, SEQ ID NO: 34 for CDR2, and SEQ ID NO: 39 for CDR3; (c) a nucleic acid molecule encoding an antibody wherein the light chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 44 for CDR1, SEQ ID NO: 46 for CDR2, and SEQ ID NO: 49 for CDR3; and the heavy chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 31 for CDR1, SEQ ID NO: 35 for CDR2, and SEQ ID NO: 40 for CDR3; (d) a nucleic acid molecule encoding an antibody wherein the light chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 44 for CDR1, SEQ ID NO: 46 for CDR2, and SEQ ID NO: 49 for CDR3; and the heavy chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 32 for CDR1, SEQ ID NO: 36 for CDR2, and SEQ ID NO: 41 for CDR3; (e) a nucleic acid molecule encoding an antibody wherein the light chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 43 for CDR1, SEQ ID NO: 45 for CDR2, and SEQ ID NO: 50 for CDR3; and the heavy chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 32 for CDR1, SEQ ID NO: 36 for CDR2, and SEQ ID NO: 41 for CDR3; (f) a nucleic acid molecule encoding an antibody wherein the light chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 43 for CDR1, SEQ ID NO: 45 for CDR2, and SEQ ID NO: 50 for CDR3; and the heavy chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 31 for CDR1, SEQ ID NO: 35 for CDR2, and SEQ ID NO: 40 for CDR3; (g) a nucleic acid molecule encoding an antibody wherein the light chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 44 for CDR1, SEQ ID NO: 46 for CDR2, and SEQ ID NO: 51 for CDR3; and the heavy chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 29 for CDR1, SEQ ID NO: 37 for CDR2, and SEQ ID NO: 42 for CDR3; (h) a nucleic acid molecule encoding an antibody wherein the light chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 44 for CDR1, SEQ ID NO: 46 for CDR2, and SEQ ID NO: 51 for CDR3; and the heavy chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 29 for CDR1, SEQ ID NO: 37 for CDR2, and SEQ ID NO: 42 for CDR3; and (i) a nucleic acid molecule encoding an antibody wherein the light chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 43 for CDR1, SEQ ID NO: 45 for CDR2, and SEQ ID NO: 52 for CDR3; and the heavy chain variable region comprises 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 29 for CDR1, SEQ ID NO: 37 for CDR2, and SEQ ID NO: 42 for CDR3.

128. A nucleic acid molecule of claim 127 encoding an antibody comprising a heavy chain constant region having an amino acid sequence of SEQ ID NO: 25, SEQ ID NO: 26, or SEQ ID NO: 28.

129. A nucleic acid molecule of claim 127 encoding an antibody comprising a light chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 43 for CDR1, SEQ ID NO: 45 for CDR2, and SEQ ID NO: 48 for CDR3; and a heavy chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 30 for CDR1, SEQ ID NO: 34 for CDR2, and SEQ ID NO: 39 for CDR3.

130. A nucleic acid molecule of claim 129 encoding an antibody comprising a heavy chain constant region having the amino acid sequence of SEQ ID NO: 28.

131. A nucleic acid molecule of claim 129 encoding an antibody comprising a light chain constant region having the amino acid sequence of SEQ ID NO: 23.

132. A nucleic acid molecule of claim 129 encoding an antibody comprising a heavy chain constant region having the amino acid sequence of SEQ ID NO: 28 and a light chain constant region having the amino acid sequence of SEQ ID NO: 23.

133. A nucleic acid molecule of claim 127 selected from the group consisting of:
(a) a nucleic acid molecule encoding an antibody wherein the light chain variable region has the amino acid sequence of SEQ ID NO: 1 and the heavy chain variable region has the amino acid sequence of SEQ ID NO: 2; (b) a nucleic acid molecule encoding an antibody wherein the light chain variable region has the amino acid sequence of SEQ ID NO: 3 and the heavy chain variable region has the amino acid sequence of SEQ ID NO: 4; (c) a nucleic acid molecule encoding an antibody wherein the light chain variable region has the amino acid sequence of SEQ ID NO: 5 and the heavy chain variable region has the amino acid sequence of SEQ ID NO: 6; (d) a nucleic acid molecule encoding an antibody wherein the light chain variable region has the amino acid sequence of SEQ ID NO: 7 and the heavy chain variable region has the amino acid sequence of SEQ ID NO: 8; (e) a nucleic acid molecule encoding an antibody wherein the light chain variable region has the amino acid sequence of SEQ ID NO: 9 and the heavy chain variable region has the amino acid sequence of SEQ ID NO: 10; (f) a nucleic acid molecule encoding an antibody wherein the light chain variable region has the amino acid sequence of SEQ ID NO: 11 and the heavy chain variable region has the amino acid sequence of SEQ ID NO: 12; (g) a nucleic acid molecule encoding an antibody wherein the light chain variable region has the amino acid sequence of SEQ ID NO: 13 and the heavy chain variable region has the amino acid sequence of SEQ ID NO: 14; (h) a nucleic acid molecule encoding an antibody wherein the light chain variable region has the amino acid sequence of SEQ ID NO: 15 and the heavy chain variable region has the amino acid sequence of SEQ ID NO: 16; and (i) a nucleic acid molecule encoding an antibody wherein the light chain variable region has the amino acid sequence of SEQ ID NO: 17 and the heavy chain variable region has the amino acid sequence of SEQ ID NO: 18.

134. A nucleic acid molecule of claim 133 encoding an antibody comprising a heavy chain constant region having an amino acid sequence of SEQ ID NO: 25, SEQ ID NO: 26, or SEQ ID NO: 28.

135. A nucleic acid molecule of claim 133 encoding an antibody comprising a light chain constant region having the amino acid sequence of SEQ ID NO: 23.

136. A nucleic acid molecule of claim 133 encoding an antibody comprising a heavy chain constant region having an amino acid sequence of SEQ ID NO: 25, SEQ ID NO: 26, or SEQ ID NO: 28; and a light chain constant region having the amino acid sequence of SEQ ID NO: 23.

137. A nucleic acid molecule of claim 133 encoding an antibody comprising a light chain variable region having the amino acid sequence of SEQ ID NO: 3 and a heavy chain variable region having the amino acid sequence of SEQ ID NO: 4.

138. A nucleic acid molecule of claim 137 encoding an antibody comprising a heavy chain constant region having the amino acid sequence of SEQ ID NO: 28.

139. A nucleic acid molecule of claim 137 encoding an antibody comprising a light chain constant region having the amino acid sequence of SEQ ID NO: 23.

140. A nucleic acid molecule of claim 137 encoding an antibody comprising a heavy chain constant region having the amino acid sequence of SEQ ID NO: 28 and a light chain constant region having the amino acid sequence of SEQ ID NO: 23.

141. A nucleic acid molecule encoding an antibody that binds to P-selectin wherein the antibody comprises a light chain variable region having an amino acid sequence selected from the group consisting of: SEQ ID NO: 1; SEQ ID NO: 3; SEQ ID NO: 5; SEQ ID NO: 7; SEQ ID NO: 9; SEQ ID NO: 11; SEQ ID NO: 13; SEQ ID NO: 15; SEQ ID NO: 17; SEQ ID NO: 19; and SEQ ID NO: 21; or a heavy chain variable region having an amino acid sequence selected from the group consisting of: SEQ ID NO: 2; SEQ ID NO: 4; SEQ ID NO: 6; SEQ ID NO: 8; SEQ ID NO: 10; SEQ ID NO: 12; SEQ ID NO: 14; SEQ ID NO: 16; SEQ ID NO: 18; SEQ ID NO: 20; and SEQ ID NO: 22.

142. A nucleic acid molecule of claim 141 encoding an antibody comprising a light chain variable region having the amino acid sequence of SEQ ID NO: 3.

143. A nucleic acid molecule of claim 141 encoding an antibody comprising a heavy chain variable region having the amino acid sequence of SEQ ID NO: 4.

144. A nucleic acid molecule encoding an antibody that binds to P-selectin selected from the group consisting of:
(a) a nucleic acid molecule encoding an antibody wherein the light chain variable region of the antibody comprises the amino acid sequences of the 3 complementarity determining regions in SEQ ID NO: 19; and the heavy chain variable region of the antibody comprises the amino acid sequences of the 3 complementarity determining regions in SEQ ID NO: 20; and (b) a nucleic acid molecule encoding an antibody wherein the light chain variable region of the antibody comprises the amino acid sequences of the 3 complementarity determining regions in SEQ ID NO: 21; and the heavy chain variable region of the antibody comprises the amino acid sequences of the 3 complementarity determining regions in SEQ ID NO: 22.

145. A nucleic acid molecule of claim 144 encoding an antibody comprising a light chain variable region comprising the 3 complementarity determining regions in SEQ ID NO: 19; and a heavy chain variable region comprising the 3 complementarity determining regions in SEQ ID NO: 20.

146. A nucleic acid molecule of claim 144 encoding an antibody comprising a light chain variable region comprising the 3 complementarity determining regions in SEQ ID NO: 21; and a heavy chain variable region comprising the 3 complementarity determining regions in SEQ ID NO: 22.

147. A nucleic acid molecule of claim 127 encoding an antibody comprising a light chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 43 for CDR1, SEQ ID NO: 45 for CDR2, and SEQ ID NO: 47 for CDR3; and a heavy chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 29 for CDR1, SEQ ID NO: 33 for CDR2, and SEQ ID NO: 38 for CDR3.

148. A nucleic acid molecule of claim 127 encoding an antibody comprising a light chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 44 for CDR1, SEQ ID NO: 46 for CDR2, and SEQ ID NO: 49 for CDR3; and a heavy chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 31 for CDR1, SEQ ID NO: 35 for CDR2, and SEQ ID NO: 40 for CDR3.

149. A nucleic acid molecule of claim 127 encoding an antibody comprising a light chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 44 for CDR1, SEQ ID NO: 46 for CDR2, and SEQ ID NO: 49 for CDR3; and a heavy chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 32 for CDR1, SEQ ID NO: 36 for CDR2, and SEQ ID NO: 41 for CDR3.

150. A nucleic acid molecule of claim 127 encoding an antibody comprising a light chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 43 for CDR1, SEQ ID NO: 45 for CDR2, and SEQ ID NO: 50 for CDR3; and a heavy chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 32 for CDR1, SEQ ID NO: 36 for CDR2, and SEQ ID NO: 41 for CDR3.

151. A nucleic acid molecule of claim 127 encoding an antibody comprising a light chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 43 for CDR1, SEQ ID NO: 45 for CDR2, and SEQ ID NO: 50 for CDR3; and a heavy chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 31 for CDR1, SEQ ID NO: 35 for CDR2, and SEQ ID NO: 40 for CDR3.

152. A nucleic acid molecule of claim 127 encoding an antibody comprising a light chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 44 for CDR1, SEQ ID NO: 46 for CDR2, and SEQ ID NO: 51 for CDR3; and a heavy chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 29 for CDR1, SEQ ID NO: 37 for CDR2, and SEQ ID NO: 42 for CDR3.

153. A nucleic acid molecule of claim 127 encoding an antibody comprising a light chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 44 for CDR1, SEQ ID NO: 46 for CDR2, and SEQ ID NO: 51 for CDR3; and a heavy chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 29 for CDR1, SEQ ID NO: 37 for CDR2, and SEQ ID NO: 42 for CDR3.

154. A nucleic acid molecule of claim 127 encoding an antibody comprising a light chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 43 for CDR1, SEQ ID NO: 45 for CDR2, and SEQ ID NO: 52 for CDR3; and a heavy chain variable region comprising 3 complementarity determining regions having the amino acid sequences of: SEQ ID NO: 29 for CDR1, SEQ ID NO: 37 for CDR2, and SEQ ID NO: 42 for CDR3.

155. A vector comprising a nucleic acid molecule of claim 127.

156. A host cell comprising a vector of claim 155.

157. A vector comprising a nucleic acid molecule of claim 129.

158. A host cell comprising a vector of claim 157.

159. A vector comprising a nucleic acid molecule of claim 130.

160. A host cell comprising a vector of claim 159.

161. A vector comprising a nucleic acid molecule of claim 137.

162. A host cell comprising a vector of claim 161.

163. A vector comprising a nucleic acid molecule of claim 140.

164. A host cell comprising a vector of claim 163.

165. A vector comprising a nucleic acid molecule of claim 142.

166. A host cell comprising a vector of claim 165.

167. A vector comprising a nucleic acid molecule of claim 143.

168. A host cell comprising a vector of claim 167.

169. A nucleic acid molecule encoding an antibody that binds to P-selectin comprising a light chain variable region, a light chain constant region, a heavy chain variable region, and a heavy chain constant region, wherein the light chain variable region has the amino acid sequence of SEQ ID NO:3, the light chain constant region has the amino acid sequence of SEQ ID NO:23, the heavy chain variable region has the amino acid sequence of SEQ ID NO:4, and the heavy chain constant region has the amino acid sequence of SEQ ID NO:28.

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