Genetically engineered plant cells and plants exhibiting resistance to glutamine synthetase inhibitors, DNA fragments and recombinants for use in the production of said cells and plants

Abstract

The invention relates to a DNA fragment containing a determined gene, the expression of which inhibits the antibiotic and herbicidal effects of Bialaphos and related products. It also relates to recombinant vectors, containing such DNA fragment, which enable this protective gene to be introduced and expressed into cells and plant cells.

The invention claimed is: 
     
       1. An isolated DNA encoding a protein having phosphinothricin acetyltransferase activity, or a variant thereof retaining said activity, said protein comprising the amino acid sequence (SEQ ID No. 1): 
       
         
           
                 
                 
               
                     
                   X   Ser Pro Glu Arg Arg Pro Ala Asp 
                 
                     
                 
                     
                   Ile Arg Arg Ala Thr Glu Ala Asp MET 
                 
                     
                 
                     
                   Pro Ala Val Cys Thr Ile Val Asn His 
                 
                     
                 
                     
                   Tyr Ile Glu Thr Ser Thr Val Asn Phe 
                 
                     
                 
                     
                   Arg Thr Glu Pro Gln Glu Pro Gln Glu 
                 
                     
                 
                     
                   Trp Thr Asp Asp Leu Val Arg Leu Arg 
                 
                     
                 
                     
                   Glu Arg Tyr Pro Trp Leu Val Ala Glu 
                 
                     
                 
                     
                   Val Asp Gly Glu Val Ala Gly Ile Ala 
                 
                     
                 
                     
                   Tyr Ala Gly Pro Trp Lys Ala Arg Asn 
                 
                     
                 
                     
                   Ala Tyr Asp Trp Thr Ala Glu Ser Thr 
                 
                     
                 
                     
                   Val Tyr Val Ser Pro Arg His Gln Arg 
                 
                     
                 
                     
                   Thr Gly Leu Gly Ser Thr Leu Tyr Thr 
                 
                     
                 
                     
                   His Leu Leu Lys Ser Leu Glu Ala Gln 
                 
                     
                 
                     
                   Gly Phe Lys Ser Val Val Ala Val Ile 
                 
                     
                 
                     
                   Gly Leu Pro Asn Asp Pro Ser Val Arg 
                 
                     
                 
                     
                   Met His Glu Ala Leu Gly Tyr Ala Pro 
                 
                     
                 
                     
                   Arg Gly Met Leu Arg Ala Ala Gly Phe 
                 
                     
                 
                     
                   Lys His Gly Asn Trp His Asp Val Gly 
                 
                     
                 
                     
                   Phe Trp Gln Leu Asp Phe Ser Leu Pro 
                 
                     
                 
                     
                   Val Pro Pro Arg Pro Val Leu Pro Val 
                 
                     
                 
                     
                   Thr Glu Ile 
                 
             
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
               
            
           
         
       
       in which X is Met or Val, said DNA consisting of between 549 and 625 nucleotides wherein X is encoded by ATG. 
     
     
       2. The isolated DNA of  claim 1 , consisting of the nucleotide sequence (SEQ ID No. 2 from nucleotide 2 to nucleotide 549): 
       
         
           
                 
                 
               
                     
                   NTG AGC CCA GAA CGA CGC CCG GCC GAC 
                 
                     
                     
                 
                     
                   ATC CGC CGT GCC ACC GAG GCG GAC ATG 
                 
                     
                     
                 
                     
                   CCG GCG GTC TGC ACC ATC GTC AAC CAC 
                 
                     
                     
                 
                     
                   TAC ATC GAG ACA AGC ACG GTC AAC TTC 
                 
                     
                     
                 
                     
                   CGT ACC GAG CCG CAG GAA CCG CAG GAG 
                 
                     
                     
                 
                     
                   TGG ACG GAC GAC CTC GTC CGT CTG CGG 
                 
                     
                     
                 
                     
                   GAG CGC TAT CCC TGG CTC GTC GCC GAG 
                 
                     
                     
                 
                     
                   GTG GAC GGC GAG GTC GCC GGC ATC GCC 
                 
                     
                     
                 
                     
                   TAC GCG GGC CCC TGG AAG GCA CGC AAC 
                 
                     
                     
                 
                     
                   GCC TAC GAC TGG ACG GCC GAG TCG ACC 
                 
                     
                     
                 
                     
                   GTG TAC GTC TCC CCC CGC CAC CAG CGG 
                 
                     
                     
                 
                     
                   ACG GGA CTG GGC TCC ACG CTC TAC ACC 
                 
                     
                     
                 
                     
                   CAC CTG CTG AAG TCC CTG GAG GCA CAG 
                 
                     
                     
                 
                     
                   GGC TTC AAG AGC GTG GTC GCT GTC ATC 
                 
                     
                     
                 
                     
                   GGG CTG CCC AAC GAC CCG AGC GTG CGC 
                 
                     
                     
                 
                     
                   ATG CAC GAG GCG CTC GGA TAT GCC CCC 
                 
                     
                     
                 
                     
                   CGC GGC ATG CTG CGG GCG GCC GGC TTC 
                 
                     
                     
                 
                     
                   AAG CAC GGG AAC TGG CAT GAC GTG GGT 
                 
                     
                     
                 
                     
                   TTC TGG CAG CTG GAC TTC AGC CTG CCG 
                 
                     
                     
                 
                     
                   GTA CCG CCC CGT CCG GTC CTG CCC GTC 
                 
                     
                     
                 
                     
                   ACC GAG ATC 
                 
             
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
               
            
           
         
       
       in which N is A or G.