Synthetic mycotoxin adsorbents and methods of making and utilizing the same

Abstract

The present invention relates generally to molecularly imprinted polymers (MIPs). In particular, the present invention relates to reusable, ecologically friendly MIPs that can be produced in relatively large quantities, methods of producing the same, and methods of utilizing the same (e.g., to sequester and/or adsorb target compounds (e.g., mycotoxins)). Compositions and methods of the invention find use in a variety of applications including dietary therapeutic, prophylactic, food and beverage processing and manufacture, as well as research, quality control and traceability applications.

What is claimed is: 
     
       1. A composition comprising a molecularly imprinted polymer synthesized using a mycotoxin template selected from the group consisting having a formula of N-(2-hydroxy-3,5-dichlorobenzoyl)-L-phenylalanine, 5-chloro-6,7-dimethoxy-1-methylisatine, ethyl 3-hydroxy-6,7-dimethoxy-2-indolone-3-carboxylate, 6,7-dimethoxyisatine, and 6,7-dimethoxy-1-methylisatine. 
     
     
       2. The composition of  claim 1 , wherein the mycotoxin template is N-(2-hydroxy-3,5-dichlorobenzoyl)-L-phenylalanine. 
     
     
       3. The composition of  claim 1 , wherein the mycotoxin template is 5-chloro-6,7-dimethoxy-1-methylisatine. 
     
     
       4. The composition of  claim 1 , wherein the molecularly imprinted polymer is made by providing N-(2-hydroxy-3,5-dichlorobenzoyl)-L-phenylalanine template and one or more monomers and one or more crosslinkers; and contacting the mycotoxin template with the one or more monomers and the one or more crosslinkers under conditions that permit polymerization of the one or more monomers and the one or more crosslinkers in the presence of the template. 
     
     
       5. The composition of  claim 4 , wherein the one or more monomers are selected from the group consisting of 2-vinylpyridine, 2-hydroxyethylmethacrylate and methacrylic acid.im  4 , wherein the one or more crosslinkers comprise ethylene 
     
     
       6. The composition of claglycol dimethacrylate. 
     
     
       7. The A composition of  claim 1 , comprising a molecularly imprinted polymer synthesized using a mycotoxin  wherein the molecularly imprinted polymer is made by providing N-(2-hydroxy-3,5-dichlorobenzoyl)-L-phenylalanine template selected from the group consisting of 5-chloro-6,7-dimethoxy-1-methylisatine, ethyl 3-hydroxy-6,7-dimethoxy-2-indolone-3-carboxylate, 6,7-dimethoxyisatine, and 6,7-dimethoxy-1-methylisatine; and one or more monomers and one or more crosslinkers; and contacting the mycotoxin template with the one or more monomers and the one or more crosslinkers under conditions that permit polymerization of the one or more monomers and the one or more crosslinkers in the presence of the template. 
     
     
       8. The composition of  claim 7 , wherein the one or more monomers are selected from the group consisting of 2-vinylpyridine, 2-hydroxyethylmethacrylate and methacrylic acid. 
     
     
       9. The composition of  claim 7 , wherein the one or more crosslinkers comprise ethylene glycol dimethacrylate. 
     
     
       10. A composition comprising a molecularly imprinted polymer synthesized using a mycotoxin template selected from the group consisting of 5-chloro-6,7-dimethoxy-1-methylisatine, ethyl 3-hydroxy-6,7-dimethoxy-2-indolone-3-carboxylate, 6,7-dimethoxyisatine, and 6,7-dimethoxy-1-methylisatine. 
     
     
       11. The composition of claim 10, wherein the molecularly imprinted polymer is made by providing the mycotoxin template and one or more monomers and one or more crosslinkers; and contacting the mycotoxin template with the one or more monomers and the one or more crosslinkers under conditions that permit polymerization of the one or more monomers and the one or more crosslinkers in the presence of the template.