Antibodies to troponin I and methods of use thereof

Abstract

The subject invention relates to antibodies to troponin I as well as methods of use thereof. In particular, such antibodies may be used to detect Troponin I in a patient and may also be used in the diagnosis of, for example, a myocardial infarction or acute coronary syndrome.

What is claimed is: 
     
       1. A Chinese Hamster Ovary (CHO) cell line, referred to as TnI 19C7 AM1 hG1CHO 204, designated by American Type Culture Collection (ATCC) deposit Number PTA-9816. 
     
     
       2. An isolated nucleic acid molecule encoding a binding protein, wherein the binding protein comprises a variable heavy chain, and wherein the amino acid sequence of the variable heavy chain of said binding protein has at least 90% identity to SEQ ID NO:25 and wherein said binding protein maintains the binding activity of SEQ ID NO:25. 
     
     
       3. The isolated nucleic acid molecule of  claim 2 , wherein the encoded binding protein further comprises a variable light chain, and wherein the amino acid sequence of the variable light chain of said binding protein has at least 90% identity to SEQ ID NO:28 and wherein said binding protein maintains the binding activity of SEQ ID NO:28. 
     
     
       4. An isolated nucleic acid molecule encoding a binding protein, wherein the binding protein comprises a variable heavy chain, and wherein the amino acid sequence of the variable heavy chain of said binding protein is SEQ ID NO:25. 
     
     
       5. An isolated nucleic acid molecule encoding a binding protein, wherein the binding protein comprises a variable light chain, and wherein the amino acid sequence of the variable light chain of said binding protein is SEQ ID NO:28. 
     
     
       6. The isolated nucleic acid molecule of  claim 5 , wherein said encoded binding protein further comprises a variable heavy chain, and wherein the amino acid sequence of the variable heavy chain of said binding protein is SEQ ID NO:25. 
     
     
       7. A vector comprising said isolated nucleic acid molecule wherein said isolated nucleic acid molecule encodes a binding protein, wherein said binding protein comprises the variable heavy chain of  claim 4  and the variable light chain of  claim 5 . 
     
     
       8. An isolated host cell comprising said vector of  claim 7 . 
     
     
       9. A method of producing a binding protein capable of binding to Troponin I, comprising culturing said host cell of  claim 8  for a time and under conditions sufficient to produce said binding protein. 
     
     
       10. A method of producing a Troponin I binding protein comprising culturing a host cell referred to as TnI 19C7 AM1 hG1 CHO 204, designated by American Type Culture Collection (ATCC) deposit Number PTA-9816 under conditions sufficient to produce said binding protein.  
     
     
       11. A method of producing a Troponin I binding protein comprising culturing a host cell that comprises a nucleic acid that encodes an antibody heavy chain that comprises a CDR1 sequence of SEQ ID NO:52, a CDR2 sequence of SEQ ID NO: 53, and a CDR3 sequence of SEQ ID NO:54, and a light chain that comprises a CDR1 sequence of SEQ ID NO:55, a CDR2 sequence of SEQ ID NO: 56, and a CDR3 sequence of SEQ ID NO:57 under conditions sufficient to produce said binding protein.  
     
     
       12. A method of producing a Troponin I binding protein comprising culturing a host cell that comprises a nucleic acids that encode the same heavy and light chains as the Chinese Hamster ovary cell referred to as TnI 19C7 AM1 hG1 CHO 204, designated by American Type Culture Collection (ATCC) deposit Number PTA-9816 under conditions sufficient to produce said binding protein.