Optimized FC variants

Abstract

A variant of a parent polypeptide including an Fc region, which variant exhibits increased binding to FcRn as compared to the parent polypeptide and includes at least one amino acid modification in the Fc region.

The invention claimed is: 
     
       1. A variant of a parent polypeptide comprising a Fc region, which variant exhibits increased binding to FcRn as compared to said parent polypeptide and comprises
 a combination of amino acid modifications selected from the group consisting of: 
 N315D/A330V/N361D/A378V/N434Y, 
 V264E/N315D/A378V/N390S/G420R/N434Y, 
 N315D/A378V/N434Y, 
 N315D/A330V/A378V/N434Y, 
 N315D/K334E/A378V/N434Y, 
 V264E/N315D/A378V, 
 P228R/N315D/A330V/N361D/A378V/N434Y, 
 P228R/P230S/N315D/A330V/N361D/A378V/N434Y, 
 P228L/N315D/A330V/N361D/A378V/N434Y, 
 P228L/P230S/N315D/A330V/N361D/A378V/N434Y, 
 P230S/N315D/A330V/N361D/A378V/N434Y, 
 P230T/V264E/N315D/K370R/A378V, 
 of the Fc region, wherein the numbering of the amino acids in the Fc region is that of the EU index as in Kabat. 
 
     
     
       2. The variant according to  claim 1 , wherein said variant is an antibody. 
     
     
       3. The variant according to  claim 2 , wherein said antibody is an IgG antibody. 
     
     
       4. A pharmaceutical composition comprising a variant as defined in  claim 1 . 
     
     
       5. A medicament comprising a variant according to  claim 1 . 
     
     
       6. An isolated nucleic acid encoding a variant as defined in  claim 1 . 
     
     
       7. A vector comprising the nucleic acid of  claim 6 . 
     
     
       8. An isolated host cell containing the vector of  claim 7 . 
     
     
       9. A method for producing a variant according to  claim 1 , comprising culturing a host cell containing a vector comprising an isolated nucleic acid encoding said variant so that the nucleic acid is expressed. 
     
     
       10. A variant according to claim 1, wherein the Fc region of the parent polypeptide is SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, or SEQ ID NO: 4.