Genomic plant sequences and uses thereof

Abstract

The present invention discloses rice genomic promoter sequences. The promoters are particularly suited for use in rice and other cereal crops. Methods of modifying, producing, and using the promoters are also disclosed. The invention further discloses compositions, transformed host cells, transgenic plants, and seeds containing the rice genomic promoter sequences, and methods for preparing and using the same.

We claim: 
     
       1. A substantially purified nucleic acid molecule that comprises the nucleotide sequence of SEQ ID NO: 1, or a complement thereof, operably linked to a heterologous structural nucleic acid sequence. 
     
     
       2. A substantially purified nucleic acid molecule that consists of the nucleotide sequence of SEQ ID NO: 1, or a complement thereof, operably linked to a heterologous structural nucleic acid sequence. 
     
     
       3. A substantially purified nucleic acid molecule comprising a nucleic acid sequence wherein the nucleic acid sequence:
 i) hybridizes under high stringeny conditions with the sequence of SEQ ID NO:1 or a complement thereof; or 
 ii) exhibits an 85% or greater identity to the sequence of SEQ ID NO:1; 
 wherein the nucleic acid sequence is operably linked to a heterologous structural nucleic acid squence. 
 
     
     
       4. The nucleic acid molecule of  claim 3 , wherein the nucleic acid sequence exhibits a 90% or greater identity to the nucleic acid sequence of SEQ ID NO:1. 
     
     
       5. The nucleic acid molecule of  claim 3 , wherein the nucleic acid sequence exhibits a 95% or greater identity to the nucleic acid sequence of SEQ D NO:1. 
     
     
       6. The nucleic acid molecule of  claim 3 , wherein the nucleic acid sequence exhibits a 99% or greater identity to the nucleic acid sequence of SEQ ID NO:1. 
     
     
       7. The nucleic acid molecule of  claim 3 , wherein said nucleic acid sequence comprises the sequence of SEQ ID NO: 1. 
     
     
       8. The nucleic acid sequence of  claim 3 , wherein the nucleic acid molecule further comprises one or more cis-acting nucleic acid elements. 
     
     
       9. The nucleic acid molecule of  claim 3 , wherein the nucleic acid molecule further comprises a 5′ leader sequence selected from the group consisting of dSSU 5′, PetHSP70 5′, and GmHSP17.9 5′. 
     
     
       10. The nucleic acid molecule of  claim 3 , wherein the nucleic acid molecule further comprises a 3′ untranslated region. 
     
     
       11. The nucleic acid molecule of  claim 10 , wherein the 3′ untranslated region is selected from the group consisting of NOS 3′, E9 3′, ADR12 3′, 7Sα3′, 11S 3′, and albumin 3′. 
     
     
       12. A transgenic plant comprising a recombinant nucleic acid molecule having the nucleic acid sequence of  claim 3 . 
     
     
       13. A host cell comprising a recombinant nucleic acid molecule having the nucleic acid molecule of  claim 3 . 
     
     
       14. The host cell of  claim 13 , wherein said host cell is a plant cell. 
     
     
       15. A transgenic plant comprising the host cell of  claim 13 .