Immunoreactive protein orthologs of Ehrlichia canis and E. chaffeensis

Abstract

The present invention concerns gp36 immunoreactive compositions for E. canis and gp 47 immunoreactive compositions for E. chaffeensis. In particular, epitopes for E. canis gp36 and E. chaffeensis gp 47 are disclosed. In certain embodiments, the immunoreactive compositions comprise tandem repeats having carbohydrate moieties.

What is claimed is: 
     
       1. A composition comprising one or more isolated polypeptides, said polypeptides having a sequence comprising (a) SEQ ID NO:22, SEQ ID NO:45 or SEQ ID NO:46; (b) SEQ ID NO:23 or SEQ ID NO:24; or (c) a sequence that is at least 90% identical to one or more sequences in (a) or (b), wherein the polypeptide is one or more isolated polypeptides are 100 amino acid residues or less in length, wherein said one or more polypeptides are bound to a solid matrix or to an immobilizing surface. 
     
     
       2. The composition of  claim 1 , wherein the polypeptide is one or more polypeptides are chemically synthesized. 
     
     
       3. The composition of  claim 1 , further comprising a pharmaceutically acceptable excipient. 
     
     
       4. The composition of  claim 1 , wherein one or more of the polypeptide comprises polypeptides comprise two or more carbohydrate moieties. 
     
     
       5. The composition of  claim 1 , comprising wherein the one or more isolated polypeptides having have a sequence comprising (a) SEQ ID NO:22, SEQ ID NO:45 or SEQ ID NO:46; or (b) SEQ ID NO:23 or SEQ ID NO:24. 
     
     
       6. The composition of  claim 5 , wherein the polypeptide at least one of the polypeptides comprises SEQ ID NO:22. 
     
     
       7. The composition of  claim 5 , wherein the polypeptide at least one of the polypeptides comprises SEQ ID NO:45. 
     
     
       8. The composition of  claim 5 , wherein the polypeptide at least one of the polypeptides comprises SEQ ID NO:46. 
     
     
       9. The composition of  claim 5 , wherein the polypeptide at least one of the polypeptides comprises SEQ ID NO:23. 
     
     
       10. The composition of  claim 5 , wherein the polypeptide at least one of the polypeptides comprises SEQ ID NO:24. 
     
     
       11. The composition of claim 1, wherein the solid matrix is an immunoblot.  
     
     
       12. The composition of claim 11, wherein the immunoblot is a nitrocellulose membrane.  
     
     
       13. The composition of claim 1, wherein one or more of the polypeptides are bound to a ELISA plate.  
     
     
       14. The composition of claim 1, wherein one or more of the polypeptides are bound to a dot blot.  
     
     
       15. The composition of claim 1, wherein one or more of the polypeptides are bound to a western blot.  
     
     
       16. The composition of claim 1, wherein one or more of the polypeptides are bound to an immobilizing surface, further defined as the surface of an ELISA plate.