P
USRE49293EExpiredUtilityPatentIndex 72

Cell culture methods and devices utilizing gas permeable materials

Assignee: WILSON WOLF MFGPriority: Oct 8, 2003Filed: Jun 12, 2018Granted: Nov 15, 2022
Est. expiryOct 8, 2023(expired)· nominal 20-yr term from priority
Inventors:WILSON JOHN RPAGE DOUGLAS AWELCH DANIELROBECK ALISON
C12M 23/08C12M 25/14C12M 23/24C12N 2500/02C12N 5/0602C12N 5/00C12M 25/06
72
PatentIndex Score
1
Cited by
270
References
30
Claims

Abstract

Gas permeable devices and methods are disclosed for cell culture, including cell culture devices and methods that contain medium at heights, and certain gas permeable surface area to medium volume ratios. These devices and methods allow improvements in cell culture efficiency and scale up efficiency.

Claims

exact text as granted — not AI-modified
What is claimed is: 
     
       1. A method of culturing animal cells in a cell culture device
 comprising a single compartment, at least the bottom of said single compartment being comprised at least in part of a non porous gas permeable material, said gas permeable material is in contact with ambient gas; the method comprising 
 adding medium and animal cells into said cell culture device; and 
 placing said cell culture device in a cell culture location that includes ambient gas at a composition for cell culture, wherein said cell culture device is oriented in a position such that the uppermost location of said medium is more than 2.0 cm higher than the lowest location of said medium, and the animal cells within said device is are in a state of static cell culture. 
 
     
     
       2. The method of  claim 1  wherein said medium is at least 2.5 cm higher than the lowest location of said medium. 
     
     
       3. The method of  claim 1  wherein said medium is at least 3.2 cm higher than the lowest location of said medium. 
     
     
       4. The method of  claim 1  wherein said medium is at least 4.0 cm higher than the lowest location of said medium. 
     
     
       5. The method of  claim 1  wherein said medium is at least 5.09 cm higher than the lowest location of said medium. 
     
     
       6. The method of  claim 1  wherein said medium is at least 7.0 cm higher than the lowest location of said medium. 
     
     
       7. The method of  claim 1  wherein a portion of said medium resides in an area not directly above the cells being cultured. 
     
     
       8. The method of  claim 1  wherein said cell culture device is further comprising at least one gas permeable material support in contact with said non porous gas permeable material. 
     
     
       9. The method of  claim 1  wherein said compartment includes a first sidewall comprised at least in part of non porous gas permeable material and wherein, at some point in time after said state of static culture has been initiated, said cell culture device is oriented into a position in which cells gravitate to said first sidewall. 
     
     
       10. A method of culturing animal cells comprising:
 a. adding medium and animal cells into a cell culture device, the cell culture device comprising a single compartment, at least the bottom of said single compartment being comprised at least in part of non porous gas permeable material that is in contact with ambient gas; and 
 b. placing said cell culture device in a cell culture location that includes ambient gas at a composition for cell culture, wherein said cell culture device is oriented in a position such that the said bottom is horizontal, the uppermost location of said medium is beyond 2.0 cm above the lowest location of said medium, and the animal cells within said device is are in a state of static cell culture. 
 
     
     
       11. The method of  claim 10  wherein said medium is at least 2.5 cm above the lowest location of said medium. 
     
     
       12. The method of  claim 10  wherein said medium is at least 3.2 cm above the lowest location of said medium. 
     
     
       13. The method of  claim 10  wherein said medium is at least 4.0 cm above the lowest location of said medium. 
     
     
       14. The method of  claim 10  wherein said medium is at least 5.09 cm above the lowest location of said medium. 
     
     
       15. The method of  claim 10  wherein said medium is at least 7.0 cm above the lowest location of said medium. 
     
     
       16. The method of  claim 10  wherein said medium is at least 8.0 cm above the lowest location of said medium. 
     
     
       17. The method of  claim 10  wherein said medium is at least 9.0 cm above the lowest location of said medium. 
     
     
       18. The method of  claim 10  wherein said medium is at least 10.2 cm above the lowest location of said medium. 
     
     
       19. A method of culturing animal cells in a cell culture device
 comprising a single compartment, at least the bottom of said single compartment being comprised at least in part of non porous gas permeable material that is in contact with ambient gas; the method comprising: 
 adding medium and animal cells into said cell culture device; and 
 placing said cell culture device in a cell culture location that includes ambient gas at a composition for cell culture, wherein said cell culture device is oriented in a position such that the said bottom is horizontal, the uppermost location of said medium is beyond 2.0 cm above the lowest location of said medium, and the animal cells within said device is and said device is are  not subjected to mixing or perfusion. 
 
     
     
       20. The method of  claim 19  wherein said medium is at least 2.5 cm above the lowest location of said medium. 
     
     
       21. The method of  claim 19  wherein said medium is at least 3.2 cm above the lowest location of said medium. 
     
     
       22. The method of  claim 19  wherein said medium is at least 4.0 cm above the lowest location of said medium. 
     
     
       23. The method of  claim 19  wherein said medium is at least 5.09 cm above the lowest location of said medium. 
     
     
       24. The method of  claim 19  wherein said medium is at least 7.0 cm above the lowest location of said medium. 
     
     
       25. The method of  claim 19  wherein said medium is at least 8.0 cm above the lowest location of said medium. 
     
     
       26. The method of  claim 19  wherein said medium is at least 9.0 cm above the lowest location of said medium. 
     
     
       27. The method of  claim 19  wherein said medium is at least 10.2 cm above the lowest location of said medium. 
     
     
       28. A method of culturing animal cells comprising:
 adding medium and animal cells into a cell culture device comprising a single compartment, at least the bottom of said single compartment being comprised at least in part of non porous gas permeable material that is in contact with ambient gas and performing static cell culture with the device in a cell culture location that includes ambient gas at a composition for animal cell culture, wherein said bottom is horizontal, the uppermost location of said medium is beyond 2.0 cm above the lowest location of said medium, and the animal cells within said device is are not subjected to mixing or perfusion. 
 
     
     
       29. The method of  claim 28  wherein said medium is at least 3.2 cm above the lowest location of said medium. 
     
     
       30. The method of  claim 28  wherein said medium is at least 5.09 cm above the lowest location of said medium.

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