Fine particle measuring apparatus

Abstract

A fine particle measuring apparatus is provided. The fine particle measuring apparatus includes a detection unit configured to detect light emitted from a fine particle and a processing unit having a memory device storing instructions which when executed by the processing unit, cause the processing unit to calculate a corrected intensity value of the detected light and generate spectrum data based on the corrected intensity value.

The invention is claimed as follows: 
     
       1. An information processing apparatus comprising:
 a processor; and   a memory device storing instructions which when executed by the processor, cause the processor to generate spectrum data based on intensity value corresponding to light from a sample detected by a plurality of light receiving elements,   wherein the spectrum data is generated based on a corrected intensity value calculated by using the intensity value and at least one of a detection wavelength bandwidth and a detection frequency range of each of the plurality of light receiving elements.   
     
     
       2. The information processing apparatus of  claim 1 , wherein the sample is a fine particle. 
     
     
       3. The information processing apparatus of  claim 1 , wherein the plurality of light receiving elements include different detection wavelength bands. 
     
     
       4. The information processing apparatus of  claim 1 , wherein the corrected intensity value includes a first corrected intensity value and the processor further calculates a second corrected intensity value based on the first corrected intensity value and relative sensitivity data of each corresponding one of the plurality of light receiving elements. 
     
     
       5. The information processing apparatus of  claim 1 , wherein the memory device storing instructions which when executed by the processor, cause the processor to compare the spectrum data with reference spectrum data. 
     
     
       6. The information processing apparatus of  claim 1 , wherein the processor is configured to form a spectrum chart in which an axis expresses a detection wavelength and another axis expresses the corrected intensity value, so as to output the spectrum chart to a display. 
     
     
       7. The information processing apparatus of  claim 6 , wherein the corrected intensity value in the spectrum chart is expressed by at least one of a statistical number including an average value, a standard error and a medium value, and a quartile point depending on a number of samples. 
     
     
       8. The information processing apparatus of  claim 6 , wherein the spectrum chart is configured to be displayed as a three dimensional graph. 
     
     
       9. An analyzing apparatus comprising:
 a detection unit having a plurality of light receiving elements configured to detect light emitted from a sample;   a processor; and   a memory device storing instructions which when executed by the processor, cause the processor to generate spectrum data based on intensity value corresponding to the detected light,   wherein the spectrum data is generated based on a corrected intensity value calculated by using the intensity value and at least one of a detection wavelength bandwidth and a detection frequency range of each of the plurality of light receiving elements.   
     
     
       10. The analyzing apparatus of  claim 9 , wherein the sample is a fine particle. 
     
     
       11. The analyzing apparatus of  claim 9 , wherein the plurality of light receiving elements includes different detection wavelength bands. 
     
     
       12. The analyzing apparatus of  claim 9 , further comprising a display,
 wherein the memory device storing instructions which when executed by the processor, cause the display to display a spectrum chart in which an axis expresses a detection wavelength and another axis expresses the corrected intensity value.   
     
     
       13. The analyzing apparatus of  claim 12 , wherein the corrected intensity value in the spectrum chart is expressed by at least one of a statistical number including an average value, a standard error and a medium value, and a quartile point depending on a number of samples. 
     
     
       14. The analyzing apparatus of  claim 12 , wherein the spectrum chart is configured to be displayed as a three dimensional graph. 
     
     
       15. The analyzing apparatus of  claim 9 , wherein the corrected intensity value is a first corrected intensity value and the process is configured to further calculate a second corrected intensity value based on the first corrected intensity value and relative sensitivity data of each corresponding one of the plurality of light receiving elements. 
     
     
       16. The analyzing apparatus of  claim 9 , wherein the memory device storing instructions which when executed by the processor, cause the processor to compare the spectrum data with reference spectrum data. 
     
     
       17. The analyzing apparatus of  claim 16 , further comprising a display,
 wherein the memory device storing instructions which when executed by the processor, cause the processor to display results of the comparison of the spectrum data with the reference spectrum data.   
     
     
       18. A flow cytometer configured to generate spectrum data based on intensity values of light from a plurality of fine particles, comprising:
 a light source configured to irradiate light to the fine particles;   a plurality of detectors including at least a first detector and a second detector, the plurality of detectors configured to detect the light from the fine particles in plural wavelength regions; and   one or more optical elements arranged to receive the light from the fine particles and to determine detection wavelength bandwidths of the light detected at the plurality of detectors,   wherein the first detector receives a longer wavelength of the light from the one or more optical elements than the second detector, and wherein the first detector has a wider detection wavelength bandwidth of the light from the one or more optical elements than the second detector.    
     
     
       19. The flow cytometer according to claim 18, wherein the first detector or a third detector of the plurality of detectors, which is a longer wavelength detector than the second detector, is configured to detect a wider detection wavelength bandwidth than the detection wavelength bandwidth of the second detector.  
     
     
       20. The flow cytometer according to claim 18, wherein the detectors include a plurality of channel numbers, and wherein a larger channel number represents a longer detection wavelength.  
     
     
       21. The flow cytometer according to claim 18, wherein the flow cytometer is configured to generate a spectrum having a channel number axis and an intensity value axis.  
     
     
       22. The flow cytometer according to claim 21, wherein the spectrum is configured to be displayed as a two dimensional graph.  
     
     
       23. The flow cytometer according to claim 21, wherein the spectrum is configured to be displayed as a three dimensional graph.  
     
     
       24. The flow cytometer according to claim 18, wherein the flow cytometer is configured to generate a spectrum having a detection wavelength axis and an intensity value axis.  
     
     
       25. The flow cytometer according to claim 18, further comprising a display configured to display detected data in each one of the plural wavelength regions.  
     
     
       26. The flow cytometer according to claim 25, wherein the detected data in each one of the plural wavelength regions has a plurality of colors, and a respective color at each intensity in each wavelength region indicates a number of the fine particles.  
     
     
       27. The flow cytometer according to claim 18, wherein the intensity value includes at least one of statistical numbers including an average value, a standard error and a medium value, and a quartile point depending on a number of fine particles.  
     
     
       28. The flow cytometer according to claim 18, wherein the fine particles include one or more of a cell, microorganism, liposome, polymer, latex particle, gel particle, or industrial particle.  
     
     
       29. The flow cytometer according to claim 18, wherein the flow cytometer is configured to compare the spectrum data with reference spectrum data.  
     
     
       30. The flow cytometer according to claim 18, wherein the fine particles include one or more of single dyed particles, non-dyed particles or multi-dyed particles.  
     
     
       31. The flow cytometer according to claim 18, wherein the flow cytometer is configured to generate a corrected spectrum based on a spectrum of non-dyed fine particles.  
     
     
       32. A flow cytometer system comprising:
 a display; and   a flow cytometer configured to generate spectrum data based on intensity values of light from a plurality of fine particles,   wherein the flow cytometer includes:
 a light source configured to irradiate light to the fine particles, 
 a plurality of detectors including at least a first detector and a second detector, the plurality of detectors configured to detect the light from the fine particles in plural wavelength regions, and 
 one or more optical elements arranged to receive the light from the fine particles and to determine detection wavelength bandwidths of the light detected at the plurality of detectors, 
 wherein the display is configured to display detected data in each one of the plural wavelength regions, and 
 wherein the first detector receives a longer wavelength of the light from the one or more optical elements than the second detector, and wherein the first detector has a wider detection wavelength bandwidth of the light from the one or more optical elements than the second detector.  
   
     
     
       33. The flow cytometer system according to claim 32, wherein the first detector or a third detector of the plurality of detectors, which is a longer wavelength detector than the second detector, is configured to detect a wider detection wavelength bandwidth than the detection wavelength bandwidth of the second detector.  
     
     
       34. The flow cytometer system according to claim 32, wherein the detectors include a plurality of channel numbers, and wherein a larger channel number represents a longer detection wavelength.  
     
     
       35. The flow cytometer system according to claim 32, wherein the flow cytometer is configured to generate a spectrum having a channel number axis and an intensity value axis.  
     
     
       36. The flow cytometer system according to claim 35, wherein the spectrum is configured to be displayed as a two dimensional graph.  
     
     
       37. The flow cytometer system according to claim 35, wherein the spectrum is configured to be displayed as a three dimensional graph.  
     
     
       38. The flow cytometer system according to claim 32, wherein the detected data in each one of the plural wavelength regions has a plurality of colors, and a respective color at each intensity in each wavelength region indicates a number of the fine particles.  
     
     
       39. The flow cytometer system according to claim 32, wherein the flow cytometer is configured to compare the spectrum data with reference spectrum data.  
     
     
       40. The flow cytometer system according to claim 32, wherein the fine particles include one or more of single dyed particles, non-dyed particles or multi-dyed particles.  
     
     
       41. The particle analyzing system according to claim 32, wherein the flow cytometer is configured to generate a corrected spectrum based on a spectrum of non-dyed fine particles.  
     
     
       42. A method, comprising:
 irradiating light, by a light source, to a plurality of fine particles;   receiving, by one or more optical elements, the light from the fine particles;   determining, by the one or more optical elements, detection wavelength bandwidths of the light at a plurality of detectors;   detecting the light, by the plurality of detectors, emitted from the fine particles in plural wavelength regions; and   generating spectrum data, by a flow cytometer, based on intensity values of light from the fine particles,   wherein the detectors include at least a first detector and a second detector,   wherein the first detector receives a longer wavelength of the light from the one or more optical elements than the second detector, and wherein the first detector has a wider detection wavelength bandwidth of the light from the one or more optical elements than the second detector.    
     
     
       43. A non-transitory computer readable medium storing instructions which, when executed, are configured to cause a flow cytometer to:
 irradiate light, by a light source, to a plurality of fine particles;   receive, by one or more optical elements, the light from the fine particles;   determining, by the one or more optical elements, detection wavelength bandwidths of the light at a plurality of detectors;   detect the light, by the plurality of detectors, emitted from the fine particles in plural wavelength regions; and   generate spectrum data based on intensity values of light from the fine particles,   wherein the detectors include at least a first detector and a second detector,   wherein the first detector receives a longer wavelength of the light from the one or more optical elements than the second detector, and wherein the first detector has a wider detection wavelength bandwidth of the light from the one or more optical elements than the second detector.