Pretreatment method of an automatic analyzer

Abstract

A pretreatment method of an automatic analyzer, in which pretreatments of a plurality of substances to be measured can be performed by a series of treatments using a plurality of magnetic beads that can bind to the plurality of substances to be measured, is achieved. In the pretreatment method of an automatic analyzer, a magnetic bead is added to a sample containing a substance to be measured, the substance to be measured is bound to the magnetic bead, the magnetic bead is extracted from the sample, and the substance to be measured is separated from the magnetic bead by an eluate. A plurality of magnetic beads that bind to a plurality of types of substances to be measured are added to a sample, and the plurality of types of substances to be measured are extracted from the magnetic bead by an eluate.

What is claimed is: 
     
       1. A method of pretreating a sample for an automatic analyzer comprising the steps of:
 in one assay protocol, which includes a plurality of cycles in a predetermined time: 
 adding first magnetic beads to the sample, which contains a plurality of substances to be measured, the first magnetic beads each having surfaces modified with an antibody as a functional group that specifically binds to a structure of a first substance to be measured among the plurality of substances to be measured in the sample; 
 adding second magnetic beads to the sample, the second magnetic beads each having surfaces modified with an octadecylsilyl group that specifically binds to a second substance to be measured among the plurality of substances to be measured in the sample; 
 extracting the first magnetic beads and the second magnetic beads from the sample; and 
 separating, by a first eluate, the first substance to be measured from the first magnetic beads; 
 transferring the first eluate to a mass spectrometer; and 
 after transferring the first eluate to the mass spectrometer, separating, by a second eluate, the second substance to be measured from the second magnetic beads, 
 wherein each step is performed in one or more of the cycles of the one assay protocol. 
 
     
     
       2. The method of pretreating the sample for an automatic analyzer according to  claim 1 ,
 wherein the first eluate corresponds to the first magnetic beads and the second eluate, which is different than the first eluate, corresponds to the second magnetic beads. 
 
     
     
       3. The method of pretreating the sample for an automatic analyzer according to  claim 1 ,
 wherein the first magnetic beads and the second magnetic beads are housed in a measuring reagent container, 
 wherein concentrations of the first magnetic beads and the second magnetic beads are based on blood concentrations of the plurality of types of substances to be measured contained in the sample. 
 
     
     
       4. The method of pretreating the sample for an automatic analyzer  according to 1 ,
 wherein the first magnetic beads and the second magnetic beads are housed in a measuring reagent container, 
 wherein addition amounts of the first magnetic beads and the second magnetic beads are based on blood concentrations of the plurality of types of substances to be measured contained in the sample. 
 
     
     
       5. A method of pretreating a sample for an automatic analyzer comprising the steps of:
 in one assay protocol, which includes a plurality of cycles in a predetermined time: 
 adding first magnetic beads to the sample, which contains a plurality of substances to be measured, the first magnetic beads each having surfaces modified with an antibody as a functional group that specifically binds to a structure of a first substance to be measured among the plurality of substances to be measured in the sample; 
 adding second magnetic beads to the sample, the second magnetic beads each having surfaces modified with a reverse phase mode functional group that specifically binds to a second substance to be measured among the plurality of substances to be measured in the sample; 
 extracting the first magnetic beads and the second magnetic beads from the sample; and 
 separating, by a first eluate, the first substance to be measured from the first magnetic beads; 
 transferring the first eluate to a mass spectrometer; and 
 after transferring the first eluate to the mass spectrometer, separating, by a second eluate, the second substance to be measured from the second magnetic beads, 
 wherein each step is performed in one or more of the cycles of the one assay protocol.