Method for producing matrices of addressed ligands on a carrier

Abstract

The invention concerns a method for fabricating matrices of addressed ligands on a carrier. According to this method, an element is used such as a reservoir ( 1 ) filled with ligand and containing an electrode ( 3 ) to deposit and electrochemically fix the ligand to the conductive carrier ( 7 ). The ligand may be an oligonucleotide or a peptide, and fixing may be obtained by electropolymerisation of this oligonucleotide or peptide carrying a pyrrole group at 5′ with pyrrole.

1 - 15 . (canceled)  
     
     
         16 . Device for producing a matrix of ligands on a conductive carrier or on conductive zones of a carrier, comprising: 
 at least one ligand dispensing means ( 1 ) provided with a conductive part ( 3 ),    means for connecting firstly the conductive carrier ( 7 ) or conductive zones ( 13 ) of the carrier, and secondly the conductive part ( 3 ) of the dispensing means to an electric generator, and    means for positioning and/or moving the carrier and/or the dispenser means relative to one another and to place them in contact such as to carry out several ligand deposits on the carrier at different sites.    
     
     
         17 . Device according to  claim 16 , in which said dispensing means comprises a reservoir ( 1 ) containing the ligand and at least one electrode ( 3 ,  5 ) arranged in said reservoir and forming the conductive part of said means.  
     
     
         18 . Device according to  claim 17 , which comprises several ligand dispensing means assembled in the form of a print head.  
     
     
         19 . Device for producing a matrix of ligands on a conductive carrier or on conductive zones of a carrier, comprising: 
 an electrode ( 15 ) in wire or needle form able to be charged externally with said ligand,    means for connecting firstly the conductive carrier ( 7 ) or conductive zones ( 13 ) of the carrier, and secondly the electrode ( 15 ) to an electric generator, and    means for positioning and/or moving the carrier and/or electrode ( 15 ) relative to one another such as to carry out several ligand deposits on the carrier at different sites.