Separation column and liquid chromotograph using the same

Abstract

The flow rate of a solvent is reduced as a separation column and the separation performance is improved even under high-pressure conditions. There is provided a separation column including a monolith rod into which a sample and a mobile phase flow, the separation column comprising: a coating material coated on the outer circumference of a monolith rod; a support member into which the monolith rod coated with the coating material is inserted, and a rod fixing material fitted into or filled a gap between the coating material and the support member; wherein the upper end face of the rod fixing material is sealed, the upper end face being the inflow-side end when the separation column is assembled.

1 . A monolith type separation column including a monolith rod which is cylindrically formed with a porous material and into which a sample and a mobile phase flow, the separation column comprising:
 a coating material coated on the outer circumference of the monolith rod;   a support member into which the monolith rod coated with the coating material is inserted;   a rod fixing material filling a gap between the coating material and the support member;   a sealing member for preventing said sample from flowing into said rod fixing material, said sealing member contacting an outer peripheral surface of said coating material, an inflow-side end surface of said rod fixing material, an outflow-side end surface of said rod fixing material, and said support member to seal said inflow-side end surface and said outflow-side end surface of said rod fixing material; and   a push-on member for pushing and pressing said sealing member toward said outer peripheral surface of said coating material, said inflow-side end surface of said rod fixing material, said outflow-side end surface of said rod fixing material, and said support member;   wherein said rod fixing material is formed by inserting a fluid substance between said coating material and said support member and by hardening said fluid substance, and   
     wherein said rod fixing material filling said gap between said coating material and said support member is a molded solid member or a solid member processed after molding, which is made longer than said support member by 1 to 5 mm and compressed when said separation column is assembled. 
   
   
       2 . A monolith type separation column including a monolith rod which is cylindrically formed with a porous material and into which a sample and a mobile phase flow, the separation column comprising:
 a coating material coated on the outer circumference of the monolith rod and radially expandable at an inflow side end and an outflow side end of the monolith rod;   a support member into which the monolith rod coated with the coating material is inserted;   a rod fixing material filling a gap between the coating material and the support member;   a sealing member for preventing said sample from flowing into said rod fixing material, said sealing member contacting a radially expanded peripheral surface of said coating material, such that the radially expanded coating material contacts an inflow-side end surface of said rod fixing material, an outflow-side end surface of said rod fixing material, and said support member to seal said inflow-side end surface and said outflow-side end surface of said rod fixing material; and   a push-on member for pushing and pressing said sealing member toward said outer peripheral surface of said coating material, said inflow-side end surface of said rod fixing material, said outflow-side end surface of said rod fixing material, and said support member;   wherein said rod fixing material is formed by inserting a fluid substance between said coating material and said support member and by hardening said fluid substance, and   
     wherein said coating material, which is radially expandable at the end, coats said upper end faces of said rod fixing material and said support member when said separation column is assembled. 
   
   
       3 . A liquid chromatograph that uses a monolith type separation column including a monolith rod into which a sample and a mobile phase flow,
 wherein the separation column comprises:   a coating material coated on the outer circumference of the monolith rod;   a support member into which the monolith rod coated with the coating material is inserted;   a rod fixing material filling a gap between the coating material and the support member;   a sealing member for preventing said sample from flowing into said rod fixing material, said sealing member contacting an outer peripheral surface of said coating material, an inflow-side end surface of said rod fixing material, an outflow-side end surface of said rod fixing material, and said support member to seal said inflow-side end surface and said outflow-side end surface of said rod fixing material; and   a push-on member for pushing and pressing said sealing member toward said outer peripheral surface of said coating material, said inflow-side end surface of said rod fixing material, said outflow-side end surface of said rod fixing material, and said support member;   wherein said rod fixing material is formed by inserting a fluid substance between said coating material and said support member and by hardening said fluid substance; and   wherein said rod fixing material filling said gap between said coating material and said support member is a molded solid member or a solid member processed after molding, which is made longer than said support member by 1 to 5 mm and compressed when said separation column is assembled.   
   
   
       4 . A liquid chromatograph according to  claim 3 , wherein the maximum pressure of the mobile phase flowing in the monolith rod is set to 5 to 30 Mpa. 
   
   
       5 . A liquid chromatograph according to  claim 3 ,
 wherein the monolith rod is provided, on an upstream side thereof, with a diffusion member for introducing a sample to the monolith rod while allowing diffusion of the sample;   wherein the monolith rod has a diameter of 1.8 to 2.8 mm and a length of 30 mm to 200 mm; and   wherein the outer diameter of the diffusion member is at least 0.8 times and at most 1.2 times the outer diameter of the monolith rod.   
   
   
       6 . A liquid chromatograph according to  claim 3 , wherein:
 the coating material is composed of a heat shrinkable tube or a fluid resin material, the rod fixing material is composed of silicon rubber or a thermoset resin, and the support member is composed of a stainless material.   
   
   
       7 . A liquid chromatograph that uses a monolith type separation column including a monolith rod into which a sample and a mobile phase flow,
 wherein the separation column comprises:   a coating material coated on the outer circumference of the monolith rod and radially expandable at an inflow side end and an outflow side end of the monolith rod;   a support member into which the monolith rod coated with the coating material is inserted;   a rod fixing material filling a gap between the coating material and the support member;   a sealing member for preventing said sample from flowing into said rod fixing material, said sealing member contacting a radially expanded peripheral surface of said coating material, such that the radially expanded coating material contacts an inflow-side end surface of said rod fixing material, an outflow-side end surface of said rod fixing material, and said support member to seal said inflow-side end surface and said outflow-side end surface of said rod fixing material; and   a push-on member for pushing and pressing said sealing member toward said outer peripheral surface of said coating material, said inflow-side end surface of said rod fixing material, said outflow-side end surface of said rod fixing material, and said support member;   wherein said rod fixing material is formed by inserting a fluid substance between said coating material and said support member and by hardening said fluid substance; and   wherein said coating material, which is radially expandable at the end, coats said upper end faces of said rod fixing material and said support member when said separation column is assembled.   
   
   
       8 . A liquid chromatograph according to  claim 7 , wherein the maximum pressure of the mobile phase flowing in the monolith rod is set to 5 to 30 Mpa. 
   
   
       9 . A liquid chromatograph according to  claim 7 ,
 wherein the monolith rod is provided, on an upstream side thereof, with a diffusion member for introducing a sample to the monolith rod while allowing diffusion of the sample;   wherein the monolith rod has a diameter of 1.8 to 2.8 mm and a length of 30 mm to 200 mm; and   wherein the outer diameter of the diffusion member is at least 0.8 times and at most 1.2 times the outer diameter of the monolith rod.   
   
   
       10 . A liquid chromatograph according to  claim 7 , wherein:
 the coating material is composed of a heat shrinkable tube or a fluid resin material, the rod fixing material is composed of silicon rubber or a thermoset resin, and the support member is composed of a stainless material.