IN Vivo Incorporation of Unnatural Amino Acids

Abstract

The invention provides methods and compositions for in vivo incorporation of unnatural amino acids. Also provided are compositions including proteins with unnatural amino acids.

1 - 47 . (canceled) 
     
     
         48 . A method for producing in an in vitro translation system comprising a cell extract, at least one protein comprising at least one unnatural amino acid, the method comprising:
 providing the translation system with at least one nucleic acid comprising at least one selector codon, wherein the nucleic acid encodes the at least one protein;   providing the translation system with an orthogonal tRNA (O-tRNA), wherein the O-tRNA functions in the translation system and wherein the O-tRNA recognizes the at least one selector codon;   providing the translation system with an orthogonal aminoacyl tRNA synthetase (O—RS), wherein the O-RS preferentially aminoacylates the O-tRNA with the at least one unnatural amino acid in the translation system wherein the O-RS is selected from the group consisting of SEQ ID NO: 35-64; and   providing the translation system with at least one unnatural amino acid, thereby producing in the translation system the at least one protein comprising the at least one unnatural amino acid.   
     
     
         49 .- 66 . (canceled) 
     
     
         67 . The method of  claim 48  wherein the unnatural amino acid is selected from the group consisting of: an O-methyl-L-tyrosine, a 3-methyl-phenylalanine, an O-4-allyl-L-tyrosine, an isopropyl-L-phenylalanine, a p-azido-L-phenylalanine, a p-acyl-L-phenylalanine, a p-benzoyl-L-phenylalanine, a p-bromophenylalanine, a p-amino-L-phenylalanine, and L-3-(2-naphthyl)alanine. 
     
     
         68 .- 70 . (canceled) 
     
     
         71 . The method of  claim 48 , wherein the O-tRNA comprises a nucleic acid comprising a polynucleotide sequence selected from the group consisting of: SEQ ID NO: 1-3 and a complementary polynucleotide sequence thereof. 
     
     
         72 . The method of  claim 48  wherein the at least one selector codon is a nonsense codon, a rare codon, or a four base codon. 
     
     
         73 . The method of  claim 72  wherein the nonsense codon is an amber codon. 
     
     
         74 .- 75 . (canceled) 
     
     
         76 . The method of  claim 48 , wherein the O-RS comprises a polypeptide encoded by a nucleic acid comprising a polynucleotide sequence selected from the group consisting of: SEQ ID NO: 4-32 and a complementary polynucleotide sequence thereof. 
     
     
         77 .- 140 . (canceled)