Recombinant avian infectious coryza vaccine and process for preparing same

Abstract

A recombinant avian infectious coryza vaccine and a process for preparing the same are provided. A process for preparing a recombinant avian infectious coryza vaccine which comprises step of constructing E. coli that may produce as an inclusion body a fusion peptide consisting of peptides derived from outer-membrane protein of Avibacterium paragarinarum serotype A and serotype C, step of culturing said E. coli and colleting and purifying inclusion body from culture, and step of preparing a preparation comprising said purified inclusion body, and an avian infectious coryza vaccine comprising as an active ingredient the fusion peptide. A linker sequence may be inserted between the respective peptides comprising the fusion peptide. For the peptide derived from the serotypes A and C, an amino acid sequence region of Region 2 or its vicinity responsible for protection from infection may be used.

1 - 19 . (canceled) 
     
     
         20 . A recombinant avian infectious coryza vaccine comprising as an active ingredient a peptide consisting of a sequence comprising the amino acid sequence shown by SEQ ID NO: 35, said sequence being within the amino acid sequence shown by SEQ ID NO: 1 with addition of 1 to 200 amino acid residues at the N-terminal and/or C-terminal thereof. 
     
     
         21 . The vaccine of  claim 20  wherein said vaccine comprises as an active ingredient a peptide consisting of the amino acid sequence shown by SEQ ID NO: 1. 
     
     
         22 - 23 . (canceled) 
     
     
         24 . The vaccine of  claim 21  or  23  wherein said peptide comprises an amino acid sequence where one or several amino acids are deleted, added or replaced.