Immunoglobulin purification

Abstract

The current invention reports a method for purifying an immunoglobulin, wherein the method comprises applying an aqueous, buffered solution comprising an immunoglobulin in monomeric, in aggregated, and in fragmented form to an anion exchange chromatography material under conditions whereby the immunoglobulin in monomeric form does not bind to the anion exchange material, and recovering the immunoglobulin in monomeric form in the flow-through from the anion exchange chromatography material, whereby the buffered aqueous solution has a pH value of from 8.0 to 8.5. In one embodiment the anion exchange chromatography material is a membrane anion exchange chromatography material.

1 - 4 . (canceled) 
     
     
         5 . A method for obtaining an immunoglobulin in monomeric form, wherein the method comprises the following step:
 a. applying an aqueous, buffered solution comprising an immunoglobulin in monomeric and in aggregated form and/or immunoglobulin fragments to an anion exchange chromatography material,   wherein the aqueous, buffered solution has a pH value of from pH 8.0 to pH 8.5, and   whereby the immunoglobulin depleted of immunoglobulin aggregates and immunoglobulin fragments is recovered from the flow-through of the anion exchange chromatography material and thereby an immunoglobulin in monomeric form is obtained.   
     
     
         6 . The method according to  claim 5 , characterized in that said anion exchange chromatography material is a membrane anion exchange chromatography material. 
     
     
         7 . The method according to  claim 5 , characterized in that said anion exchange chromatography material is a strong anion exchange chromatography material. 
     
     
         8 . The method according  claim 5 , characterized in that said method comprises prior to step a) an additional protein A chromatography step or a HCIC chromatography step. 
     
     
         9 . The method according to  claim 8 , characterized in that said anion exchange chromatography material is a membrane anion exchange chromatography material. 
     
     
         10 . The method according to  claim 8 , characterized in that said anion exchange chromatography material is a strong anion exchange chromatography material.