Fabrication of patterned arrays

Abstract

Provided herein are methods and compositions for the fabrication of patterned arrays, such as nucleotide arrays. The methods and compositions are suited for the transfer and reorientation of array components.

1 .- 41 . (canceled) 
     
     
         42 . A method for generating an array comprising:
 providing a template array comprising a plurality of template oligonucleotides coupled thereto;   coupling the template array to a recipient array comprising a plurality of oligonucleotides complementary to portions of the plurality of template oligonucleotides; and   generating a recipient array comprising recipient oligonucleotide products, wherein each of the recipient oligonucleotide products comprises a member of the plurality of oligonucleotides and an additional oligonucleotide, wherein the additional oligonucleotide is complementary to an additional portion of a member of the plurality of template oligonucleotides.   
     
     
         43 . The method of  claim 42 , wherein at least 40% of the recipient oligonucleotide products are complementary to a full-length oligonucleotide from the plurality of template oligonucleotides. 
     
     
         44 . The method of  claim 42 , wherein the template array comprises at least 100 spots. 
     
     
         45 . The method of  claim 42 , wherein the directionality of the recipient oligonucleotide products relative to the recipient array is the same as the directionality of the template oligonucleotides relative to the template array. 
     
     
         46 . The method of  claim 42 , wherein the directionality of the recipient oligonucleotide products relative to the recipient array is the opposite of the directionality of the template oligonucleotides relative to the template array. 
     
     
         47 . The method of  claim 42 , wherein a plurality of recipient arrays are generated. 
     
     
         48 . The method of  claim 42 , wherein the plurality of recipient oligonucleotide products are on average at least 99% identical between one recipient array and another. 
     
     
         49 . A method for generating a complementary array comprising:
 (a) providing a plurality of template oligonucleotides coupled to a first substrate, each of the plurality of template oligonucleotides comprising an adaptor sequence, wherein the adaptor sequence is the same for each of the plurality of template oligonucleotides;   (b) providing a plurality of recipient oligonucleotides coupled to a second substrate, each of the plurality of recipient oligonucleotides comprising a sequence complementary to the adaptor sequence;   (c) generating a plurality of recipient oligonucleotide products, wherein each of the recipient oligonucleotide products comprises the sequence complementary to the adaptor sequence and an additional oligonucleotide, wherein the additional oligonucleotide is complementary to a member of the plurality of template oligonucleotides.   
     
     
         50 . The method of  claim 49 , wherein each of the adaptor sequences is located at or near the 3′ end of the template oligonucleotides. 
     
     
         51 . The method of  claim 49 , wherein each of the adaptor sequences is located at or near a 5′ end of the template oligonucleotides. 
     
     
         52 . The method of  claim 49 , wherein the generating in (d) results in generation of the plurality of recipient oligonucleotide products at least 40% of which are full-length products on the second substrate. 
     
     
         53 . The method of  claim 49 , wherein the directionality of the recipient oligonucleotide products relative to the second substrate is the same as the directionality of the template oligonucleotides relative to the first substrate. 
     
     
         54 . The method of  claim 49 , wherein the directionality of the recipient oligonucleotide products relative to the second substrate is the opposite of the directionality of the template oligonucleotides relative to the first substrate. 
     
     
         55 . The method of  claim 54 , wherein the method is repeated to produce at least 2 recipient arrays. 
     
     
         56 . The method of  claim 49 , wherein the plurality of template oligonucleotides are coupled to the first substrate via a first polymerization reaction. 
     
     
         57 . The method of  claim 56 , wherein reagents for the first polymerization reaction comprise acrylamide or polyacrylamide. 
     
     
         58 . The method of  claim 49 , wherein the plurality of recipient oligonucleotide products are coupled to the second substrate via a second polymerization reaction. 
     
     
         59 . The method of  claim 58 , wherein reagents for the second polymerization reaction comprise acrylamide or polyacrylamide 
     
     
         60 . The method of  claim 49 , wherein a plurality of recipient arrays are generated. 
     
     
         61 . The method of  claim 49 , wherein the plurality of recipient oligonucleotide products are on average at least 99% identical between one recipient array and another.