Protein having nampt activity, and method for producing nmn

Abstract

A protein of the present invention is a protein having nicotinamide phosphoribosyl transferase activity, described in any one of the following [1] to [3]: [1] a protein consisting of an amino acid sequence set forth in SEQ ID NO: 3 or 5; [2] a mutant protein consisting of an amino acid sequence obtained by deleting, substituting, inserting, or adding 1 to 20 amino acids in the amino acid sequence set forth in SEQ ID NO: 3 or 5; and [3] a homologous protein consisting of an amino acid sequence having 90% or more identity to the amino acid sequence set forth in SEQ ID NO: 3 or 5.

1 . A protein having nicotinamide phosphoribosyl transferase activity, described in any one of the following [1] to [3]:
 [1] a protein consisting of an amino acid sequence set forth in SEQ ID NO: 3 or 5;   [2] a mutant protein consisting of an amino acid sequence obtained by deleting, substituting, inserting, or adding 1 to 20 amino acids in the amino acid sequence set forth in SEQ ID NO: 3 or 5; and   [3] a homologous protein consisting of an amino acid sequence having 90% or more identity to the amino acid sequence set forth in SEQ ID NO: 3 or 5.   
     
     
         2 . A DNA encoding the protein according to  claim 1 . 
     
     
         3 . The DNA according to  claim 2  that is a DNA described in any one of the following [4] to [6]:
 [4] a DNA consisting of a nucleotide sequence set forth in SEQ ID NO: 2 or 4; 
 [5] a DNA that hybridizes, under stringent conditions, a DNA consisting of a nucleotide sequence complementary to the nucleotide sequence set forth in SEQ ID NO: 2 or 4; and 
 [6] a DNA consisting of a nucleotide sequence having 90% or more identity to the nucleotide sequence set forth in SEQ ID NO: 2 or 4. 
 
     
     
         4 . A recombinant DNA comprising the DNA according to  claim 2 . 
     
     
         5 . A transformant obtained by transforming a host cell with the recombinant DNA according to  claim 4 . 
     
     
         6 . The transformant according to  claim 5  that is a transformant having increased productivity of nicotinamide mononucleotide (NMN) as compared with the host cell. 
     
     
         7 . The transformant according to  claim 5 , wherein the host cell is  E. coli.    
     
     
         8 . A method for producing NMN, comprising producing nicotinamide mononucleotide (NMN) by using the transformant according to  claim 5 . 
     
     
         9 . A method for producing NMN, comprising producing nicotinamide mononucleotide (NMN) by using the protein according to  claim 1 . 
     
     
         10 . A recombinant DNA comprising the DNA according to  claim 3 . 
     
     
         11 . A transformant obtained by transforming a host cell with the recombinant DNA according to  claim 10 . 
     
     
         12 . The transformant according to  claim 11  that is a transformant having increased productivity of nicotinamide mononucleotide (NMN) as compared with the host cell. 
     
     
         13 . The transformant according to  claim 11 , wherein the host cell is  E. coli.    
     
     
         14 . A method for producing NMN, comprising producing nicotinamide mononucleotide (NMN) by using the transformant according to  claim 11 .