Polynucleotide encoding mammalian cytokine-like polypeptide-10

Abstract

A mammalian cytokine-like polypeptide, called Mammalian Cytokine-like polypeptide-10, (Zcyto10), polynucleotides encoding the same, antibodies which specifically bind to the polypeptide, and anti-idiotypic antibodies which bind to the antibodies. Zcyto10 is useful for promoting the healing of wounds and for stimulating the proliferation of platelets.

1. An expression vector comprising the following operably linked elements:
 (a) a transcription promoter; 
 (b) a DNA segment encoding a polypeptide comprising amino acid residues 25 to 176 of SEQ ID NO:2; and 
 (c) a transcription terminator. 
 
     
     
       2. The expression vector according to  claim 1 , further comprising a secretory signal sequence operably linked to the DNA segment. 
     
     
       3. The expression vector according to  claim 1 , wherein the polypeptide comprises an affinity tag or an immunoglogulin constant region. 
     
     
       4. An expression vector comprising the following operably linked elements:
 (a) a transcription promoter; 
 (b) a DNA segment encoding a polypeptide comprising SEQ ID NO:2; and 
 (c) a transcription terminator. 
 
     
     
       5. The expression vector according to  claim 4 , further comprising a secretory signal sequence operably linked to the DNA segment. 
     
     
       6. The expression vector according to  claim 4 , wherein the polypeptide comprises an affinity tag or an iinmunoglogulin constant region. 
     
     
       7. A cultured cell into which has been introduced the expression vector according to  claim 1 , wherein said cell expresses the polypeptide encoded by the DNA segment. 
     
     
       8. A method of producing a polypeptide comprising: culturing a cell into which has been introduced the expression vector according to  claim 7 , whereby the cell expresses the polypeptide encoded by the DNA segment; and isolating the polypeptide produced by the cell. 
     
     
       9. A cultured cell into which has been introduced the expression vector according to  claim 4 , wherein said cell expresses the polypeptide encoded by the DNA segment. 
     
     
       10. A method of producing a polypeptide comprising: culturing a cell into which has been introduced the expression vector according to  claim 9 , whereby the cell expresses the polypeptide encoded by the DNA segment; and isolating the polypeptide produced by the cell. 
     
     
       11. An expression vector comprising the following operably linked elements:
 (a) a transcription promoter; 
 (b) a DNA segment encoding a polypeptide comprising SEQ ID NO:26; and 
 (c) a transcription terminator. 
 
     
     
       12. The expression vector according to  claim 11 , further comprising a secretory signal sequence operably linked to the DNA segment. 
     
     
       13. The expression vector according to  claim 11 , wherein the polypeptide comprises an affinity tag or an immunoglogulin constant region. 
     
     
       14. A cultured cell into which has been introduced the expression vector according to  claim 11 , wherein said cell expresses the polypeptide encoded by the DNA segment. 
     
     
       15. A method of producing a polypeptide comprising: culturing a cell into which has been introduced the expression vector according to  claim 14 , whereby the cell expresses the polypeptide encoded by the DNA segment; and isolating the polypeptide produced by the cell.