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US9958453B2ActiveUtilityPatentIndex 47

Biological sensing method for separating biomolecule

Assignee: UNIV NATIONAL CHIAO TUNGPriority: Mar 10, 2015Filed: Aug 5, 2016Granted: May 1, 2018
Est. expiryMar 10, 2035(~8.7 yrs left)· nominal 20-yr term from priority
Inventors:LI YAW-KUENCHANG CHIA-YULI Bor-Ran
G01N 33/56944G01N 33/6878G01N 27/4145G01N 2333/3156G01N 1/405G01N 33/68
47
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Claims

Abstract

A biological sensing device and a method for separating a biomolecule are provided. The biological sensing device includes an amino acid sequence and a signal-generating unit. The amino acid sequence includes SEQ ID NO: 1 or SEQ ID NO: 2, and is for binding with UlaG protein labeled on a biomolecule. The signal-generating unit connects to the amino acid sequence.

Claims

exact text as granted — not AI-modified
What is claimed is: 
     
       1. A method for separating a biomolecule, comprising:
 providing a sample, the sample comprising a biomolecule having L-ascorbate-6-phosphate lactonase; 
 contacting the sample with an amino acid sequence of SEQ ID NO: 1 or SEQ ID NO: 2; 
 binding the amino acid sequence with the L-ascorbate-6-phosphate lactonase, wherein SEQ ID NO: 1 or SEQ ID NO: 2 has physical contact with the L-ascorbate-6-phosphate lactonase; and 
 removing a portion of the sample not bound with the amino acid sequence to separate the biomolecule from the sample. 
 
     
     
       2. The method of  claim 1 , further comprising modifying the L-ascorbate-6-phosphate lactonase onto the biomolecule. 
     
     
       3. The method of  claim 1 , further comprising modifying the sample onto a substrate. 
     
     
       4. The method of  claim 1 , further comprising connecting the amino acid sequence to a signal-generating unit.

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