Nude mouse model for human neoplastic disease

Abstract

A nude mouse model for human neoplastic diseases having histologically intact human neoplastic tissue transplanted onto an organ of the mouse which corresponds to the human organ from which the tissue is obtained.

1. A nude mouse model for progression of human neoplastic disease, the progression of said disease being characterized by growth of a primary tumor site and metastasis to secondary tumor sites, wherein said mouse is characterized by
 having has histologically intact human neoplastic tissue of at least 1 mm 3  in size transplanted onto an organ of said mouse which corresponds to the human organ from which said tissue is originally obtained; and  
 havinghas sufficient immuno-deficiency to allow said transplanted neoplastic tissue to grow andat said primary site and metastasize to said secondary tumor sites, so as to mimic the progression of the neoplastic disease including the metastatic behavior of said neoplastic disease in the human donorhumans.  
 
     
     
       2. A nude mouse model according to  claim 1  wherein said neoplastic tissue is selected from breast tissue, ovarian tissue or and pleural tissue. 
     
     
       3. A nude mouse model according to  claim 2  wherein said neoplastic tissue is obtained from human breast tissue. 
     
     
       4. A nude mouse model according to  claim 3  wherein said human neoplastic breast tissue is implanted in the mammary fat pad of the mouse. 
     
     
       5. A nude mouse model according to  claim 2  wherein said neoplastic tissue is obtained from human ovarian tissue. 
     
     
       6. A nude mouse model according to  claim 5  wherein said human neoplastic ovarian tissue is implanted in the ovarian capsule of the mouse. 
     
     
       7. A nude mouse model according to  claim 5  wherein said human neoplastic ovarian tissue is transplanted by securing to the surface of the mouse ovary at least two pieces of neoplastic tissue in close proximity to each other. 
     
     
       8. A nude mouse model according to  claim 2  wherein said neoplastic tissue is obtained from human pleural tissue. 
     
     
       9. A nude mouse model according to  claim 8  wherein said neoplastic tissue is implanted in the parietal pleura of the mouse. 
     
     
       10. A nude mouse model according to  claim 9  wherein said neoplastic tissue is implanted in the visceral pleura of the mouse. 
     
     
       11. A method of generating a nude mouse model for progression of human neoplastic disease, the progression of said disease being characterized by growth of a primary tumor site and metastasis to secondary tumor sites, said method comprising:
 transplanting histologically intact human neoplastic tissue of at least 1 mm 3  in size transplanted onto an organ of a nude mouse which corresponds to the human organ from which said tissue is originally obtained; and  
 allowing said transplanted tissue to grow and at said primary site and metastasize to said secondary tumor sites, so as to mimic progression of the neoplastic disease including the metastatic behavior of said neoplastic disease in the human donor humans.  
 
     
     
       12. A method of generating a nude mouse according to  claim 11  wherein said human neoplastic tissue is selected from breast tissue, ovarian tissue or and pleural tissue. 
     
     
       13. A nude rodent model for progression of human neoplastic disease, the progression of said disease being characterized by growth of a primary tumor site and metastasis to secondary tumor sites, wherein said rodent has histologically intact human neoplastic tissue of at least  1  mm 3    in size transplanted onto an organ of said rodent which corresponds to the human organ from which said tissue is originally obtained; and      has sufficient immuno - deficiency to allow said transplanted neoplastic tissue to grow at said primary site and metastasize to said secondary tumor sites, so as to mimic the progression of the neoplastic disease including the metastatic behavior of said neoplastic disease in humans.      
     
     
       14. The nude rodent model for human neoplastic disease of  claim 13 , wherein said rodent is a rat.  
     
     
       15. An immunodeficient rodent model for progression of human neoplastic disease, the progression of said disease being characterized by growth of a primary tumor site and metastasis to secondary tumor sites, wherein said rodent has histologically intact human neoplastic tissue of at least  1  mm 3    in size transplanted onto an organ of said rodent which corresponds to the human organ from which said tissue is originally obtained; and      has sufficient immuno - deficiency to allow said transplanted neoplastic tissue to grow at said primary site and metastasize to said secondary tumor sites, so as to mimic the progression of the neoplastic disease including the metastatic behavior of said neoplastic disease in humans.      
     
     
       16. The immunodeficient rodent model for human neoplastic disease of  claim 15 , wherein said rodent is a rat.  
     
     
       17. The immunodeficient rodent model for human neoplastic disease of  claim 15 , wherein said rodent is a mouse.  
     
     
       18. The immunodeficient rodent model for human neoplastic disease of  claim 17 , wherein said rodent is a severe combined immunodeficient ( SCID )  mouse.    
     
     
       19. A method of generating a nude rodent model for progression of human neoplastic disease, the progression of said disease being characterized by growth of a primary tumor site and metastasis to a secondary tumor sites, said method comprising:
   transplanting histologically intact human neoplastic tissue of at least  1  mm   3    in size onto an organ of a nude rodent which corresponds to the human organ from which said tissue is originally obtained; and        allowing said transplanted tissue to grow at said primary site and metastasize to said secondary tumor sites, so as to mimic progression of the neoplastic disease including the metastatic behavior of said neoplastic disease in humans.      
     
     
       20. The method of generating a nude rodent model for human neoplastic disease of  claim 19 , wherein said rodent is a rat.  
     
     
       21. A method of generating an immunodeficient rodent model for progression of human neoplastic disease, the progression of said disease being characterized by growth of a primary tumor site and metastasis to secondary tumor sites, said method comprising:
   transplanting histologically intact human neoplastic tissue of at least  1  mm   3    in size onto an organ of an immunodeficient rodent which corresponds to the human organ from which said tissue is originally obtained; and        allowing said transplanted tissue to grow at said primary site and metastasize to said secondary tumor sites, so as to mimic progression of the neoplastic disease including the metastatic behavior of said neoplastic disease in humans.      
     
     
       22. The method of generating an immunodeficient rodent model for human neoplastic disease of  claim 21 , wherein said rodent is a rat.  
     
     
       23. The method of generating an immunodeficient rodent model for human neoplastic disease of  claim 21 , wherein said rodent is a mouse.  
     
     
       24. The method of generating an immunodeficient rodent model for human neoplastic disease of  claim 23 , wherein said rodent is a severe combined immunodeficient ( SCID )  mouse.    
     
     
       25. A nude rodent model for progression of human neoplastic disease, the progression of said disease being characterized by growth of a primary tumor site and metastasis to secondary tumor sites, wherein said rodent has histologically intact human neoplastic tissue transplanted onto an organ of said rodent which corresponds to the human organ from which said tissue is originally obtained; and
   has sufficient immuno - deficiency to allow said transplanted neoplastic tissue to grow at said primary site and metastasize to said secondary tumor sites, so as to mimic the progression of the neoplastic disease including the metastatic behavior of said neoplastic disease in humans.      
     
     
       26. An immunodeficient rodent model for progression of human neoplastic disease, the progression of said disease being characterized by growth of a primary tumor site and metastasis to secondary tumor sites, wherein said rodent has histologically intact human neoplastic tissue transplanted onto an organ of said rodent which corresponds to the human organ from which said tissue is originally obtained; and
   has sufficient immuno - deficiency to allow said transplanted neoplastic tissue to grow at said primary site and metastasize to said secondary tumor sites, so as to mimic the progression of the neoplastic disease including the metastatic behavior of said neoplastic disease in humans.      
     
     
       27. A nude rodent model according to  claim 13  wherein said neoplastic tissue is selected from breast tissue, ovarian tissue or pleural tissue.  
     
     
       28. A nude rodent model according to  claim 27  wherein said neoplastic tissue is obtained from human breast tissue.  
     
     
       29. A nude rodent model according to  claim 27  wherein said neoplastic tissue is obtained from human ovarian tissue.  
     
     
       30. A nude rodent model according to  claim 27  wherein said neoplastic tissue is obtained from human pleural tissue.  
     
     
       31. An immunodeficient rodent model according to  claim 15  wherein said neoplastic tissue is selected from breast tissue, ovarian tissue or pleural tissue.  
     
     
       32. An immunodeficient rodent model according to  claim 31  wherein said neoplastic tissue is obtained from human breast cancer.  
     
     
       33. An immunodeficient rodent model according to  claim 31  wherein said neoplastic tissue is obtained from human ovarian tissue.  
     
     
       34. An immunodeficient rodent model according to  claim 31  wherein said neoplastic tissue is obtained from human pleural tissue.  
     
     
       35. The method according to  claim 19  wherein said neoplastic tissue is selected from breast tissue, ovarian tissue or pleural tissue.  
     
     
       36. The method according to  claim 35  wherein said neoplastic tissue is obtained from human breast tissue.  
     
     
       37. The method according to  claim 35  wherein said neoplastic tissue is obtained from human ovarian tissue.  
     
     
       38. The method according to  claim 35  wherein said neoplastic tissue is obtained from human pleural tissue.  
     
     
       39. The method according to  claim 21  wherein said neoplastic tissue is selected from breast tissue, ovarian tissue or pleural tissue.  
     
     
       40. The method according to  claim 39  wherein said neoplastic tissue is obtained from human breast tissue.  
     
     
       41. The method according to  claim 39  wherein said neoplastic tissue is obtained from human ovarian tissue.  
     
     
       42. The method according to  claim 39  wherein said neoplastic tissue is obtained from human pleural tissue.  
     
     
       43. A nude rodent model according to  claim 25  wherein said neoplastic tissue is selected from breast tissue, ovarian tissue or pleural tissue.  
     
     
       44. A nude rodent model according to  claim 43  wherein said neoplastic tissue is obtained from human breast tissue.  
     
     
       45. A nude rodent model according to  claim 43  wherein said neoplastic tissue is obtained from human ovarian tissue.  
     
     
       46. A nude rodent model according to  claim 43  wherein said neoplastic tissue is obtained from human pleural tissue.  
     
     
       47. An immunodeficient rodent model according to  claim 26  wherein said neoplastic tissue is selected from breast tissue, ovarian tissue or pleural tissue.  
     
     
       48. An immunodeficient rodent model according to  claim 47  wherein said neoplastic tissue is obtained from human breast tissue.  
     
     
       49. An immunodeficient rodent model according to  claim 47  wherein said neoplastic tissue is obtained from human ovarian tissue.  
     
     
       50. An immunodeficient rodent model according to  claim 47  wherein said neoplastic tissue is obtained from human pleural tissue.