Photolabile compound and substrate for oligomer probe array with the same

Abstract

Provided is a substrate for an oligomer probe array to which a photolabile material having an acetylene derivative is directly attached or attached via a linker.

1. A photolabile compound represented by the following formula: 
       
         
           
           
               
               
           
         
       
       wherein
 R 1  is a methyl group, an amino group, a hydroxyl group, acetic ester group, a phenyl group, a diphenyl group, a trimethylsilyl group, a cyanide group, an azide group, a sulfur atom, a cycloalkyl group, a cycloalkenyl group or a heterocyclic group; 
 n is an integer from 1 to 8; 
 R 2  is a hydrogen atom or alkyl derivative; and 
 Y is a halogen atom, a hydroxyl group, a moiety represented by one of the following formulae: 
 
       
         
           
           
               
               
           
         
       
       wherein B is adenine, cytosine, guanine, thymine or uracil;
 R 3  is a hydrogen atom, an amino group, an alkyl group or a phosphine group; 
 R 4  is a hydrogen atom, a hydroxyl group, —OR 5  or —SR 5  wherein R 5  is an alkyl group, an alkenyl group, an acetal group or a silyl ether group; 
 R 6  is an alkyl group, a phenyl group, or a sulfur atom; 
 p is an integer from 0 to 5; and 
 q is an integer from 0 to 10. 
 
     
     
       2. The photolabile compound of  claim 1 , wherein R 1  is methyl, trimethysilyl, an acetic ester group, cyclohexyl, cyclohexenyl, phenyl, naphthalenyl or thiophenyl. 
     
     
       3. The photolabile compound of  claim 1 , wherein Y is 
       
         
           
           
               
               
           
         
       
       wherein
 B is adenine, cytosine, guanine, thymine or uracil; 
 R 3  is a hydrogen atom, an amino group, an alkyl group or a phosphine group; and 
 R 4  is a hydrogen atom, a hydroxyl group, —OR 5  or —SR 5  wherein R 5  is an alkyl group, an alkenyl group, an acetal group or a silyl ether group. 
 
     
     
       4. The photolabile compound of  claim 3 , wherein R 3  is a hydrogen atom or a phosphite amide group of the following formula: 
       
         
           
           
               
               
           
         
       
       wherein R 7 's may be the same or different from each other and are independently a linear or branched alkyl radical having 1 top 4 carbon atoms. 
     
     
       5. The photolabile compound of  claim 3 , wherein R 4  is an O-methyl radical, O-ethyl radical, an O-allyl radical, an O-tetrahydropyranyl radical, an O-methoxytetrahydropyranyl radical or an O-t-butyldimethylsilyl radical. 
     
     
       6. The photolabile compound of  claim 1 , wherein Y is 
       
         
           
           
               
               
           
         
       
       and q is an integer from 3 to 10. 
     
     
       7. A substrate for an oligomer probe array to which a material is directly attached or attached via a linker, the material being represented by the following formula: 
       
         
           
           
               
               
           
         
       
       wherein
 R 1  is a methyl group, an amino group, a hydroxyl group, an acetic ester group, a phenyl group, a diphenyl group, a trimethysilyl group, a cyanide group, an azide group, a sulfur atom, a cycloalkyl group, a cycloalkenyl group or a heterocyclic group; 
 n is an integer from 1 to 8; 
 R 2  is a hydrogen atom or an alkyl derivative; and 
 Y is a halogen atom, a hydroxyl group, or 
 
       
         
           
           
               
               
           
         
       
       wherein q is an integer from 0 to 10. 
     
     
       8. The substrate for an oligomer probe array of  claim 7 , wherein the substrate is nylon, nitrocellulose, a plastic film, a silicon substrate and/or soda-lime glass. 
     
     
       9. The substrate for an oligomer probe array of  claim 7 , wherein the linker is a silane linker or nano particles.