USRE41129EExpiredUtilityPatentIndex 82

Cytokine zalpha11 ligand polynucleotides

Assignee: ZYMOGENETICS INCPriority: Mar 9, 1999Filed: Feb 2, 2006Granted: Feb 16, 2010

Est. expiryMar 9, 2019(expired)· nominal 20-yr term from priority

Inventors:NOVAK JULIA E PRESNELL SCOTT R SPRECHER CINDY A FOSTER DONALD C HOLLY RICHARD D GROSS JANE A JOHNSTON JANET V NELSON ANDREW J DILLON STACEY R HAMMOND ANGELA K

C07K 14/535A01K 2217/05A01K 2267/03C07K 14/715C07K 14/52C07K 2319/55A61P 7/00A61K 38/00C07K 2317/73C07K 16/2866A61P 35/02C07K 16/24A61P 35/00A61P 37/02C07K 14/54C07K 2319/24C07K 14/5406C07K 2319/00C12N 15/8509C07K 2319/21C07K 14/001C12N 15/62C07K 2319/30A61K 38/18C12N 2799/026A01K 2227/105G01N 2333/52A01K 67/0275

PatentIndex Score

Cited by

References

142

Claims

Abstract

The present invention relates to zalpha11 Ligand polynucleotide and polypeptide molecules. The zalpha11 Ligand is a novel cytokine. The polypeptides may be used within methods for stimulating the proliferation and/or development of hematopoietic cells in vitro and in vivo. The present invention also includes methods for producing the protein, uses therefor and antibodies thereto.

Claims

exact text as granted — not AI-modified

1. An isolated polynucleotide molecule comprising a sequence of nucleotides that encode encodes a polypeptide as shown in SEQ ID NO: 2 from comprising residue 32 to residue 162 of SEQ ID NO: 2.

2. The isolated polynucleotide molecule of claim 1 , wherein the nucleotides are as shown in SEQ ID NO: 1 from nucleotide 140 to nucleotide 532 or as shown in SEQ ID NO: 3 from nucleotide 94 to nucleotide 486.

3. An isolated polynucleotide molecule comprising a sequence of nucleotides that encode encodes a polypeptide as shown in SEQ ID NO: 2 from comprising residue 1 to residue 162 of SEQ ID NO: 2.

4. The isolated polynucleotide molecule of claim 3 , wherein the nucleotides are as shown in SEQ ID NO: 1 from nucleotide 47 to nucleotide 532 or as shown in SEQ ID NO: 3 from nucleotide 1 to nucleotide 486.

5. An expression vector comprising the following operably linked elements:
(a) a transcription promoter;
(b) a DNA segment encoding a polypeptide comprising amino acid residues 32 (Gln) to 162 (Ser) of SEQ ID NO: 2; and
(c) a transcription terminator.

6. A cultured cell comprising the expression vector according to claim 5 .

7. A method of producing a polypeptide comprising culturing a cell according to claim 6 under conditions wherein the Dna DNA segment is expressed and recovering the polypeptide encoded by the DNA segment.

8. An isolated polynucleotide molecule comprising a sequence of nucleotides that encode encodes an isolated polypeptide comprising a sequence of amino acid residues sequence that is at least 90% identical to residues 41 (Gln) to 148 (Ile) as shown in SEQ ID NO:2, wherein the residue at position 44 is Asp, the residue at position 47 is Asp and the residue at position 135 is Glu, wherein the polypeptide binds a receptor as shown in SEQ ID NO:115.

9. The isolated polynucleotide molecule of claim 8 , wherein the nucleotides are as shown in SEQ ID NO: 1 from nucleotide 167 to nucleotide 490 or as shown in SEQ ID NO: 3 from nucleotide 121 to nucleotide 444.

10. The polynucleotide molecule of claim 8 , wherein the polynucleotide molecule encodes a polypeptide that stimulates proliferation of NK cells or NK cell progenitors, stimulates activation of NK cells, stimulates proliferation of T cells, stimulates proliferation of B cells stimulated with anti-CD40 antibodies, or reduces proliferation of B cells stimulated with anti-IgM antibodies.

11. An isolated polynucleotide molecule comprising a sequence of nucleotides that encode for an isolated polypeptide comprising at least 14 contiguous amino acid residues of SEQ ID NO:2, wherein said 14 contiguous amino acids of SEQ ID NO:2 stimulate an antigenic response in a mammal.

12. An expression vector comprising the following operably linked elements:
(a) a transcription promoter;
(b) a DNA segment encoding a polypeptide comprising a sequence of amino acid residues selected from the group consisting of:
(a)( i ) amino acid residues 41-56 of SEQ ID NO: 2;
(b)( ii ) amino acid residues 69-84 of SEQ ID NO: 2;
(c)( iii ) amino acid residues 92-105 of SEQ ID NO: 2; and
(d)( iv ) amino acid residues 135-148 of SEQ ID NO: 2; wherein said amino acid sequence stimulates an antigenic response in a mammal; and

13. A cultured cell comprising the expression vector according to claim 12 .

14. A method of producing a polypeptide comprising culturing a cell according to claim 13 under conditions wherein the DNA segment is expressed and recovering the polypeptide encoded by the DNA segment.

15. An expression vector comprising the following operably linked elements:
(a) a transcription promoter;
(b) a DNA segment encoding a polypeptide comprising a sequence of amino acid residues that is at least 90% identical to residues 41 (Gln) to 148 (Ile) as shown in SEQ ID NO: 2, wherein the residue at position 44 is Asp, the residue at position 47 is Asp and the residue at position 135 is Glu, wherein the polypeptide binds a receptor as shown in SEQ ID NO: 115 NO:115 ; and
(c) a transcription terminator.

16. A cultured cell comprising the expression vector according to claim 15 .

17. A method of producing a polypeptide comprising culturing a cell according to claim 16 under conditions wherein the DNA segment is expressed and recovering the polypeptide encoded by the DNA segment.

18. An isolated polynucleotide comprising a sequence of nucleotides that encode encodes a polypeptide comprising amino acid residues that are identical to residues 41 (Gln) to 148 (Ile) as shown in SEQ ID NO:2.

19. An expression vector comprising the following operably linked elements:
(a) a transcription promoter;
(b) a DNA segment comprising a nucleic acid sequence that encodes the polypeptides a polypeptide according to claim 18 ; and
(c) a transcription terminator.

20. A cultured cell comprising the expression vector according to claim 19 .

21. An isolated polynucleotide molecule comprising a sequence of nucleotides that encode encodes a polypeptide as shown in SEQ ID NO:56 from comprising residue 23 to residue 146 of SEQ ID NO: 56.

22. An isolated polynucleotide molecule comprising a sequence of nucleotides that encode encodes a polypeptide as shown in SEQ ID NO:56 from comprising residue 1 to residue 146 of SEQ ID NO: 56.

23. An isolated polynucleotide molecule comprising a sequence of nucleotides that encode for an isolated encodes a polypeptide comprising at least 14 contiguous amino acid residues of SEQ ID NO:56, wherein said polypeptide stimulates an antigenic response in a mammal.

24. An isolated polynucleotide molecule comprising a sequence of nucleotides that encode for a polypeptide comprising at least 14 contiguous amino acid residues of SEQ ID NO: 2 , wherein said 14 contiguous amino acids of SEQ ID NO: 2 , stimulate an antigenic response in a mammal.

25. An expression vector comprising the following operably linked elements:
( a ) a transcription promoter; ( b ) a DNA segment that encodes a polypeptide comprising at least 14 contiguous amino acid residues of SEQ ID NO: 2 , wherein the contiguous amino acid residues stimulate an antigenic response in a mammal; and ( c ) a transcription terminator.

26. A cultured cell comprising the expression vector of claim 25 .

27. The cultured cell of claim 26 , wherein the cultured cell is a prokaryotic cell.

28. The prokaryotic cell of claim 27 , wherein the prokaryotic cell is an E. coli cell.

29. The cultured cell of claim 26 , wherein the cultured cell is a mammalian cell.

30. The cultured cell of claim 26 , wherein the promoter is a strong promoter.

31. The cultured cell of claim 26 , wherein the promoter is a viral promoter.

32. A process comprising culturing the cell of claim 26 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

33. A process comprising culturing the cell of claim 27 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

34. A process comprising culturing the cell of claim 28 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

35. A process comprising culturing the cell of claim 29 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

36. A process comprising culturing the cell of claim 30 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

37. A process comprising culturing the cell of claim 31 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

38. An isolated polynucleotide molecule comprising a sequence of nucleotides that encodes a polypeptide comprising a sequence of amino acid residues selected from the group consisting of:
( a ) amino acid residues 41 - 56 of SEQ ID NO: 2 ; ( b ) amino acid residues 69 - 84 of SEQ ID NO: 2 ; ( c ) amino acid residues 92 - 105 of SEQ ID NO: 2 ; and ( d ) amino acid residues 135 - 148 of SEQ ID NO: 2 ; wherein the sequence of amino acid residues stimulates an antigenic response in a mammal.

39. The cultured cell of claim 13 , wherein the cultured cell is a prokaryotic cell.

40. The prokaryotic cell of claim 39 , wherein the prokaryotic cell is an E. coli cell.

41. The cultured cell of claim 13 , wherein the cultured cell is a mammalian cell.

42. The cultured cell of claim 13 , wherein the promoter is a strong promoter.

43. The cultured cell of claim 13 wherein the promoter is a viral promoter.

44. A process comprising culturing the cell of claim 39 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

45. A process comprising culturing the cell of claim 40 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

46. A process comprising culturing the cell of claim 41 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

47. A process comprising culturing the cell of claim 42 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

48. A process comprising culturing the cell of claim 43 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

49. An isolated polynucleotide molecule comprising a sequence of nucleotides that encodes a polypeptide comprising a sequence of amino acids that is at least 90 % identical to residues 41 to 148 of SEQ ID NO: 2 , wherein the polypeptide binds a zalpha 11 receptor as shown in SEQ ID NO: 115 .

50. An expression vector comprising the following operably linked elements:
( a ) a transcription promoter; ( b ) a DNA segment that encodes a polypeptide comprising a sequence of amino acids that is at least 90 % identical to residues 41 to 148 of SEQ ID NO: 2 , wherein the polypeptide binds a zalpha 11 receptor as shown in SEQ ID NO: 115 ; and ( c ) a transcription terminator.

51. A cultured cell comprising the expression vector of claim 50 .

52. The cultured cell of claim 51 , wherein the cultured cell is a prokaryotic cell.

53. The prokaryotic cell of claim 52 , wherein the prokaryotic cell is an E. coli cell.

54. The cultured cell of claim 51 , wherein the cultured cell is a mammalian cell.

55. The cultured cell of claim 51 , wherein the promoter is a strong promoter.

56. The cultured cell of claim 51 , wherein the promoter is a viral promoter.

57. A process comprising culturing the cell of claim 51 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

58. A process comprising culturing the cell of claim 52 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

59. A process comprising culturing the cell of claim 53 under conditions whereby the DNA segment is expressed and further comprising recovering polypeptide.

60. A process comprising culturing the cell of claim 54 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

61. A process comprising culturing the cell of claim 55 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

62. A process comprising culturing the cell of claim 56 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

63. An isolated polynucleotide molecule comprising a sequence of nucleotides that encodes a polypeptide comprising residues 41 to 148 of SEQ ID NO: 2 , wherein the polypeptide binds a zalpha 11 receptor as shown in SEQ ID NO: 115 .

64. An expression vector comprising the following operably linked elements:
( a ) a transcription promoter; ( b ) a DNA segment that encodes a polypeptide comprising residues 41 to 148 of SEQ ID NO: 2 , wherein the polypeptide binds a zalpha 11 receptor as shown in SEQ ID NO: 115 ; and ( c ) a transcription terminator.

65. A cultured cell comprising the expression vector of claim 64 .

66. The cultured cell of claim 65 , wherein the cultured cell is a prokaryotic cell.

67. The prokaryotic cell of claim 66 , wherein the prokaryotic cell is an E. coli cell.

68. The cultured cell of claim 65 , wherein the cultured cell is a mammalian cell.

69. The cultured cell of claim 65 , wherein the promoter is a strong promoter.

70. The cultured cell of claim 65 , wherein the promoter is a viral promoter.

71. A process comprising culturing the cell of claim 65 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

72. A process comprising culturing the cell of claim 66 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

73. A process comprising culturing the cell of claim 67 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

74. A process comprising culturing the cell of claim 68 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

75. A process comprising culturing the cell of claim 69 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

76. A process comprising culturing the cell of claim 70 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

77. An isolated polynucleotide molecule comprising a sequence of nucleotides that encodes a polypeptide comprising a sequence of amino acids that is at least 90 % identical to residues 32 to 162 of SEQ ID NO: 2 , wherein the polypeptide binds a zalpha 11 receptor as shown in SEQ ID NO: 115 .

78. An expression vector comprising the following operably linked elements:
( a ) a transcription promoter; ( b ) a DNA segment that encodes a polypeptide comprising a sequence of amino acids that is at least 90 % identical to residues 32 to 162 of SEQ ID NO: 2 , wherein the polypeptide binds a zalpha 11 receptor as shown in SEQ ID NO: 115 ; and ( c ) a transcription terminator.

79. A cultured cell comprising the expression vector of claim 78 .

80. The cultured cell of claim 79 , wherein the cultured cell is a prokaryotic cell.

81. The prokaryotic cell of claim 80 , wherein the prokaryotic cell is an E. coli cell.

82. The cultured cell of claim 79 , wherein the cultured cell is a mammalian cell.

83. The cultured cell of claim 79 , wherein the promoter is a strong promoter.

84. The cultured cell of claim 79 , wherein the promoter is a viral promoter.

85. A process comprising culturing the cell of claim 79 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

86. A process comprising culturing the cell of claim 80 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

87. A process comprising culturing the cell of claim 81 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

88. A process comprising culturing the cell of claim 82 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

89. A process comprising culturing the cell of claim 83 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

90. A process comprising culturing the cell of claim 84 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

91. The cultured cell of claim 6 , wherein the cultured cell is a prokaryotic cell.

92. The prokaryotic cell of claim 91 , wherein the prokaryotic cell is an E. coli cell.

93. The cultured cell of claim 6 , wherein the cultured cell is a mammalian cell.

94. The cultured cell of claim 6 , wherein the promoter is a strong promoter.

95. The cultured cell of claim 6 , wherein the promoter is a viral promoter.

96. A process comprising culturing the cell of claim 91 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

97. A process comprising culturing the cell of claim 92 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

98. A process comprising culturing the cell of claim 93 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

99. A process comprising culturing the cell of claim 94 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

100. A process comprising culturing the cell of claim 95 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

101. An isolated polynucleotide molecule comprising a sequence of nucleotides that encodes a polypeptide comprising a sequence of amino acids that is at least 90 % identical to residues 41 to 148 of SEQ ID NO: 2 , wherein the polypeptide stimulates proliferation of NK cells or NK cell progenitors, stimulates activation of NK cells, stimulates proliferation of T cells, stimulates proliferation of B cells stimulated with anti - CD 40 antibodies, or reduces proliferation of B cells stimulated with anti - IgM antibodies.

102. An expression vector comprising the following operably linked elements:
( a ) a transcription promoter; ( b ) a DNA segment that encodes a polypeptide comprising a sequence of amino acids that is at least 90 % identical to residues 41 to 148 of SEQ ID NO: 2 , wherein the polypeptide stimulates proliferation of NK cells or NK cell progenitors, stimulates activation of NK cells, stimulates proliferation of T cells, stimulates proliferation of B cells stimulated with anti - CD 40 antibodies, or reduces proliferation of B cells stimulated with anti - IgM antibodies; and ( c ) a transcription terminator.

103. A cultured cell comprising the expression vector of claim 102 .

104. The cultured cell of claim 103 , wherein the cultured cell is a prokaryotic cell.

105. The prokaryotic cell of claim 104 , wherein the prokaryotic cell is an E. coli cell.

106. The cultured cell of claim 103 , wherein the cultured cell is a mammalian cell.

107. The cultured cell of claim 103 , wherein the promoter is a strong promoter.

108. The cultured cell of claim 103 , wherein the promoter is a viral promoter.

109. A process comprising culturing the cell of claim 103 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

110. A process comprising culturing the cell of claim 104 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

111. A process comprising culturing the cell of claim 105 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

112. A process comprising culturing the cell of claim 106 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

113. A process comprising culturing the cell of claim 107 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

114. A process comprising culturing the cell of claim 108 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

115. An isolated polynucleotide molecule comprising a sequence of nucleotides that encodes a polypeptide comprising residues 41 to 148 of SEQ ID NO: 2 , wherein the polypeptide stimulates proliferation of NK cells or NK cell progenitors, stimulates activation of NK cells, stimulates proliferation of T cells, stimulates proliferation of B cells stimulated with anti- CD 40 antibodies, or reduces proliferation of B cells stimulated with anti - IgM antibodies.

116. An expression vector comprising the following operably linked elements:
( a ) a transcription promoter; ( b ) a DNA segment that encodes a polypeptide comprising residues 41 to 148 of SEQ ID NO: 2 , wherein the polypeptide stimulates proliferation of NK cells or NK cell progenitors, stimulates activation of NK cells, stimulates proliferation of T cells, stimulates proliferation of B cells stimulated with anti - CD 40 antibodies, or reduces proliferation of B cells stimulated with anti - IgM antibodies; and ( c ) a transcription terminator.

117. A cultured cell comprising the expression vector of claim 116 .

118. The cultured cell of claim 117 , wherein the cultured cell is a prokaryotic cell.

119. The prokaryotic cell of claim 118 , wherein the prokaryotic cell is an E. coli cell.

120. The cultured cell of claim 117 , wherein the cultured cell is a mammalian cell.

121. The cultured cell of claim 117 , wherein the promoter is a strong promoter.

122. The cultured cell of claim 117 , wherein the promoter is a viral promoter.

123. A process comprising culturing the cell of claim 117 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

124. A process comprising culturing the cell of claim 118 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

125. A process comprising culturing the cell of claim 119 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

126. A process comprising culturing the cell of claim 120 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

127. A process comprising culturing the cell of claim 121 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

128. A process comprising culturing the cell of claim 122 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

129. An isolated polynucleotide molecule comprising a sequence of nucleotides that encodes a polypeptide comprising a sequence of amino acids that is at least 90 % identical to residues 32 to 162 to SEQ ID NO: 2 , wherein the polypeptide stimulates proliferation of NK cells or NK cell progenitors, stimulates activation of NK cells, stimulates proliferation of T cells, stimulates proliferation of B cells stimulated with anti - CD 40 antibodies, or reduces proliferation of B cells stimulated with anti - IgM antibodies.

130. An expression vector comprising the following operably linked elements:
( a ) a transcription promoter; ( b ) a DNA segment that encodes a polypeptide comprising a sequence of amino acids that is at least 90 % identical to residues 32 to 162 of SEQ ID NO: 2 , wherein the polypeptide stimulates proliferation of NK cells or NK cell progenitors, stimulates activation of NK cells, stimulates proliferation of T cells, stimulates proliferation of B cells stimulated with anti - CD 40 antibodies, or reduces proliferation of B cells stimulated with anti - IgM antibodies; and ( c ) a transcription terminator.

131. A cultured cell comprising the expression vector of claim 130 .

132. The cultured cell of claim 131 , wherein the cultured cell is a prokaryotic cell.

133. The prokaryotic cell of claim 132 , wherein the prokaryotic cell is an E. coli cell.

134. The cultured cell of claim 131 , wherein the cultured cell is a mammalian cell.

135. The cultured cell of claim 131 , wherein the promoter is a strong promoter.

136. The cultured cell of claim 131 , wherein the promoter is a viral promoter.

137. A process comprising culturing the cell of claim 131 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

138. A process comprising culturing the cell of claim 132 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

139. A process comprising culturing the cell of claim 133 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

140. A process comprising culturing the cell of claim 134 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

141. A process comprising culturing the cell of claim 135 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

142. A process comprising culturing the cell of claim 136 under conditions whereby the DNA segment is expressed and further comprising recovering the polypeptide.

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